偏振敏感数字散斑干涉法用于生物组织的全场双折射测量

IF 5 2区物理与天体物理 Q1 OPTICS

Optics and Laser Technology Pub Date : 2025-04-10 DOI:10.1016/j.optlastec.2025.112941

Guangxin Gao , Haisha Niu , Sijin Wu , Cuifang Kuang , Zhaizi Xie , Haobo Feng

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引用次数: 0

摘要

双折射是与生物组织的结构和功能有关的一种内在特性。组织病理学的评价指标在该领域引起了极大的关注。我们提出了一种偏振敏感的数字散斑干涉技术，用于生物组织中全场双折射的综合测量。通过分析偏振态散斑干涉图定位主轴方位角。全场主轴方位角定位精度为2.0°。通过空间载波相位提取和图像处理技术测量相位延迟，理论分辨率为0.012 rad，根据全场相位分布计算组织双折射率值。实验结果表明，肾癌组织和正常肾组织均存在双折射现象。癌肾组织的全视野双折射范围为3.07 × 10-3 ~ 3.53 × 10-3。而正常肾组织则在2.17 × 10-3 ~ 2.46 × 10-3之间。我们的研究工作提出了一种用于组织病理学分析和检测目的的新技术。

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Polarization-sensitive digital speckle pattern interferometry for full-field birefringence measurement of biological tissues

Birefringence represents an intrinsic characteristic that is associated with the structure and function of biological tissues. The evaluation index of histopathology has garnered significant attention in the field. We present a Polarization-Sensitive Digital Speckle Pattern Interferometry technique for the comprehensive measurement of full-field birefringence in biological tissues. Principal axis azimuth angle is positioned by analyzing speckle interferogram of polarization states. The positioning accuracy of the principal axis azimuth for the full-field is 2.0°. Phase retardation is measured through spatial carrier phase extraction and image processing techniques, with a theoretical resolution of 0.012 rad. The tissue birefringence value is computed based on the full-field phase distribution. Experimental results demonstrate that birefringence exists in the renal carcinoma tissues and normal kidney tissues. The full-field birefringence in the examined carcinoma renal tissue is observed to range from 3.07 × 10^-3 to 3.53 × 10^-3. Meanwhile, that of normal renal tissue is determined to be within 2.17 × 10^-3 to 2.46 × 10^-3. Our research work presents a novel technique for histopathological analysis and detection purposes.

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来源期刊

Optics and Laser Technology 物理-光学

CiteScore

8.50

自引率

10.00%

发文量

1060

审稿时长

3.4 months

期刊介绍： Optics & Laser Technology aims to provide a vehicle for the publication of a broad range of high quality research and review papers in those fields of scientific and engineering research appertaining to the development and application of the technology of optics and lasers. Papers describing original work in these areas are submitted to rigorous refereeing prior to acceptance for publication. The scope of Optics & Laser Technology encompasses, but is not restricted to, the following areas: •development in all types of lasers •developments in optoelectronic devices and photonics •developments in new photonics and optical concepts •developments in conventional optics, optical instruments and components •techniques of optical metrology, including interferometry and optical fibre sensors •LIDAR and other non-contact optical measurement techniques, including optical methods in heat and fluid flow •applications of lasers to materials processing, optical NDT display (including holography) and optical communication •research and development in the field of laser safety including studies of hazards resulting from the applications of lasers (laser safety, hazards of laser fume) •developments in optical computing and optical information processing •developments in new optical materials •developments in new optical characterization methods and techniques •developments in quantum optics •developments in light assisted micro and nanofabrication methods and techniques •developments in nanophotonics and biophotonics •developments in imaging processing and systems