Electrochemical Approach on a Flavonoid: Miquelianin via Carbon Based Electrodes and Its Analysis from Calystegia Silvatica.

Background: Miquelianin (MIQ) is a natural phenolic compound found in various plants, including Salvia species, with potential health benefits due to its antioxidant and anti-inflammatory properties.

Objective: This study presents the investigation of electrochemical oxidation pathway and sensitive analysis of MIQ which is isolated from the aerial parts of Calystegia silvatica that grows in Trabzon, Türkiye.

Methods: Electrochemical determination methods have gained significant attention for the quantitative analysis of bioactive compounds due to their simplicity, sensitivity, and cost-effectiveness. The electrochemical determination of MIQ primarily involves the utilization of electrochemical techniques such as cyclic voltammetry and differential pulse voltammetry.

Results: Under optimum experimental conditions, calibration plot for MIQ with a limit of detection of 99.3 × 10-9 M and 145 × 10-9 M were obtained using differential pulse voltammetry for GCE and BDDE in the range of 2.0 × 10-6 to 3.6 × 10-5 M. The presented method was validated and successfully performed for the determination of the MIQ from plant extracts with excellent recovery values as 104.7, 101.8, and 102.1%.

Conclusion: This study explores the electrochemical oxidation pathway and sensitive analysis of MIQ, isolated from Calystegia silvatica in Trabzon, Türkiye. GCE and BDDE as carbon-based electrodes have been used for the sensitive analysis of MIQ in PB solution at pH 7.0 solution. MIQ was detected by GCE and BDDE with limits of 99.3 × 10-9 M and 145 × 10-9 M, respectively, within a concentration range of 2.0 × 10-6 to 3.6 × 10-5 M. The validated method demonstrated excellent recovery values of 104.7, 101.8, and 102.1% for determining MIQ from plant extracts.

Highlights: This electrochemical developed method offers promising opportunities for accurate and sensitive analysis of miquelianin. Continued research and technological advancements in this field can contribute to a deeper understanding of the electrochemical behavior of miquelianin and facilitate its practical applications in pharmaceutical, food, and nutraceutical industries, promoting its utilization as a valuable bioactive compound.