An Electrochemical Approach for a Flavonoid: Miquelianin via Carbon-Based Electrodes and Its Analysis from Calystegia silvatica.

Background: Miquelianin (MIQ) is a natural phenolic compound found in various plants, including Salvia species, with potential health benefits due to its antioxidant and anti-inflammatory properties.

Objective: This study presents an investigation of the electrochemical oxidation pathway and sensitive analysis of MIQ, which was isolated from the aerial parts of Calystegia silvatica (CS) that grows in Trabzon, Turkey.

Methods: Electrochemical determination methods have gained significant attention for the quantitative analysis of bioactive compounds due to their simplicity, sensitivity, and cost-effectiveness. The electrochemical determination of MIQ primarily involves the utilization of electrochemical techniques such as cyclic voltammetry (CV) and differential pulse voltammetry (DPV).

Results: Under optimum experimental conditions, a calibration plot for MIQ with LODs of 99.3 × 10-9 M and 145 × 10-9 M were obtained using DPV with glassy carbon electrodes (GCEs) and boron-doped diamond electrodes (BDDEs) in the range of 2.0 × 10-6 to 3.6 × 10-5 M. The presented method was validated and successfully performed for the determination of MIQ from plant extracts with excellent recovery values as 104.7, 101.8, and 102.1%.

Conclusion: This study explores the electrochemical oxidation pathway and sensitive analysis of MIQ, isolated from C. silvatica in Trabzon, Turkey. GCEs and BDDEs as carbon-based electrodes were used for the sensitive analysis of MIQ in phosphate buffer (PB) solution at pH 7.0 solution. MIQ was detected using GCEs and BDDEs with limits of 99.3 × 10-9 M and 145 × 10-9 M, respectively, within a concentration range of 2.0 × 10-6 to 3.6 × 10-5 M. The validated method demonstrated excellent recovery values of 104.7, 101.8, and 102.1% for determining MIQ from plant extracts.

Highlights: This electrochemical method offers promising opportunities for accurate and sensitive analysis of MIQ. Continued research and technological advancements in this field can contribute to a deeper understanding of the electrochemical behavior of MIQ and facilitate its practical applications in pharmaceutical, food, and nutraceutical industries, promoting its utilization as a valuable bioactive compound.