紫檀芪通过调节肠道菌群减轻艰难梭菌感染小鼠肠道屏障损伤和继发性肝脏氧化应激。

IF 5.4 1区 农林科学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
Food & Function Pub Date : 2025-03-19 DOI:10.1039/D4FO06413E
Hao Yang, Yanling Kuang, Lamei Wang, Xinru Ma, Javier A. Villafuerte Gálvez, Jing Lu, Yanfei Dai, Shimin Liu, Junhu Yao, Xinhua Chen and Yangchun Cao
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引用次数: 0

摘要

艰难梭菌感染(CDI)是一种重要的感染性疾病,治疗方案有限。紫檀芪是蓝莓中发现的一种活性化合物,以其抗氧化和抗炎特性而闻名。本研究探讨了紫檀芪对CDI致小鼠肠道屏障损伤和肝脏二次氧化应激的影响。观察大鼠结肠和肝脏的病理变化、抗炎细胞因子和抗氧化剂水平以及相关基因的表达。此外,还进行了肠道微生物群和胆汁酸的16S rRNA测序和靶向代谢组学分析。紫檀芪降低了肠球菌等有害细菌的丰度,同时增加了乳酸菌等有益细菌的数量,从而重塑了肠道菌群和胆汁酸谱,减少了T-βMCA的积累。这一过程激活了肠道FXR信号,减轻了结肠炎症,降低了肠道通透性。肠道通透性的降低阻止了细菌和细菌毒素通过门静脉转运到肝脏,从而减少肝脏炎症和氧化应激。紫芪通过调节CDI引起的生态失调和胆汁酸紊乱来维持肠道健康,是一种很有前景的策略。如果将紫檀芪纳入食品体系,就有可能改善肠道健康,减轻与受污染农产品相关的CDI风险,并加强公共卫生和食品安全。此外,我们发现调节肠道胆汁酸谱和FXR受体可以作为CDI的潜在治疗靶点,从而促进新的治疗选择和饮食策略的发展。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Pterostilbene attenuates intestinal barrier damage and secondary liver oxidative stress in a murine model of Clostridium difficile infection by regulating the gut microbiota†

Pterostilbene attenuates intestinal barrier damage and secondary liver oxidative stress in a murine model of Clostridium difficile infection by regulating the gut microbiota†

Clostridium difficile infection (CDI) is a significant infectious disease with limited treatment options. Pterostilbene, an active compound found in blueberries, is known for its antioxidant and anti-inflammatory properties. This study investigated the effects of pterostilbene on intestinal barrier damage and secondary liver oxidative stress induced by CDI in mice. Pathological changes in the colon and liver, the levels of anti-inflammatory cytokines and antioxidants, and the expression of related genes were evaluated. Additionally, 16S rRNA sequencing and targeted metabolomics analyses of the gut microbiota and bile acids were conducted. Pterostilbene reduced the abundance of harmful bacteria such as Enterococcus, while increasing beneficial bacteria like Lactobacillus, thereby reshaping the gut microbiota and bile acid profile and reducing the accumulation of T-βMCA. This process activated intestinal FXR signaling, which alleviated colonic inflammation and reduced intestinal permeability. The reduction in intestinal permeability prevented the translocation of bacteria and bacterial toxins into the liver via the portal vein, thereby reducing liver inflammation and oxidative stress. Pterostilbene presented a promising strategy for maintaining intestinal health through the regulation of dysbiosis and bile acid disturbances caused by CDI. When integrated into the food system, pterostilbene has the potential to improve intestinal health, mitigate the risk of CDI associated with contaminated agricultural products, and enhance public health and food safety. Additionally, we identified that regulating the intestinal bile acid profile and the FXR receptor could serve as potential therapeutic targets for CDI, thereby facilitating the development of novel treatment options and dietary strategies.

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来源期刊
Food & Function
Food & Function BIOCHEMISTRY & MOLECULAR BIOLOGY-FOOD SCIENCE & TECHNOLOGY
CiteScore
10.10
自引率
6.60%
发文量
957
审稿时长
1.8 months
期刊介绍: Food & Function provides a unique venue for physicists, chemists, biochemists, nutritionists and other food scientists to publish work at the interface of the chemistry, physics and biology of food. The journal focuses on food and the functions of food in relation to health.
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