神经酸在三阴性乳腺癌中的抗癌作用。

IF 1.6 4区 农林科学 Q3 CHEMISTRY, APPLIED
Akiho Kawai, Fumiaki Ono, Takao Satou, Tatsuki Itoh
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引用次数: 0

摘要

三阴性乳腺癌(TNBC)没有治疗靶点;因此,治疗仅限于抗癌药物。然而,TNBC是高度耐药的,这意味着需要新的治疗TNBC的药物。脂肪酸可以抑制和促进不同的癌症。神经酸(NA)是一种具有抗炎作用的单价不饱和脂肪酸,但其对癌症的影响尚不清楚。因此,本研究探讨NA对TNBC的影响。将TNBC细胞系BT-549细胞暴露于不同浓度的NA(1、10、100和1000µM)或对照(二甲亚砜)。此外,9周龄雌性BALB/cSlc-nu/nu小鼠将BT-549细胞移植到第4乳腺脂肪垫右侧,然后给予NA (100 mg/kg)或无菌自来水1周。增加NA浓度会降低BT-549细胞的活力。在100µM时,NA增加单链DNA的表达,单链DNA是细胞凋亡的标志;细胞凋亡抑制剂核因子-κ B表达降低;并降低增殖标志物Ki-67的表达。此外,10µM NA通过不显著增加E-cadherin (CDH1) mRNA表达,显著降低N-cadherin (CDH2) mRNA表达和基质金属蛋白酶-9和2蛋白表达,抑制BT-549细胞的迁移和侵袭。此外,na处理小鼠乳腺肿瘤的生长和肺转移明显低于对照组小鼠。提示NA在体内外均有抗肿瘤作用;因此,NA可能是一种新的治疗TNBC的药物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Anticancer Effects of Nervonic Acid in Triple-Negative Breast Cancer.

Triple-negative breast cancer (TNBC) has no therapeutic target; therefore, treatment is restricted to anticancer agents. However, TNBC is highly drug-resistant, meaning that novel therapeutic drugs for TNBC are required. Fatty acids can both inhibit and promote different cancers. Nervonic acid (NA) is a monovalent unsaturated fatty acid with anti-inflammatory effects, although its effect on cancer is unknown. Therefore, this study investigated the effect of NA on TNBC. BT-549 cells, a TNBC cell line, were exposed to different concentrations of NA (1, 10, 100, and 1000 µM) or a control (dimethylsulfoxide). In addition, 9-week-old, female BALB/cSlc-nu/nu mice had a BT-549 cell transplant into the right side of the fourth mammary fat pad before being administered NA (100 mg/kg) or sterile tap water for 1 week. Increasing NA concentrations reduced the viability of BT-549 cells. At 100 µM, NA increased the expression of single-stranded DNA, a marker of apoptosis; decreased the expression of nuclear factor-κ B, an apoptosis inhibitor; and decreased the expression of Ki-67, a marker of proliferation. Furthermore, 10 µM NA inhibited cell migration and invasion of BT-549 cells via a non-significant increase in the mRNA expression of E-cadherin (CDH1) and significant decreases in the mRNA expression of N-cadherin (CDH2) and the protein expression of matrix metalloproteinase-9 and 2. In addition, the growth and metastasis to the lungs of mammary gland tumors were significantly lower in NA-treated mice than in control mice. These results suggest that NA has an antitumor effect both in vitro and in vivo; therefore, NA may be a novel therapeutic agent for TNBC.

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来源期刊
Journal of oleo science
Journal of oleo science CHEMISTRY, APPLIED-FOOD SCIENCE & TECHNOLOGY
CiteScore
3.20
自引率
6.70%
发文量
173
审稿时长
3 months
期刊介绍: The J. Oleo Sci. publishes original researches of high quality on chemistry, biochemistry and science of fats and oils such as related food products, detergents, natural products, petroleum products, lipids and related proteins and sugars. The Journal also encourages papers on chemistry and/or biochemistry as a major component combined with biological/ sensory/nutritional/toxicological evaluation related to agriculture and/or food.
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