紫玉米花青素对鸡屠宰性能、免疫功能、盲肠菌群和转录组的影响

IF 3.8 1区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Poultry Science Pub Date : 2025-03-27 DOI:10.1016/j.psj.2025.105104

Xingzhou Tian , Chao Ban , Di Zhou , Hui Li , Jiaxuan Li , Xu Wang , Qi Lu

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引用次数: 0

摘要

在高集约饲养条件下，家禽易受氧化应激影响，从而降低免疫功能，对生产性能产生负面影响。此外，花青素还能减轻鸡的氧化应激，提高免疫功能。本试验旨在研究紫玉米花青素提取物（PCE）对鸡屠宰性能、免疫功能、盲肠菌群和转录组的影响。试验采用完全随机设计，将180只雏鸡随机分为基础饲粮（CON）组和添加360 mg/kg PCE的处理（PCE）组。结果显示，PCE治疗组血浆免疫球蛋白A、免疫球蛋白G、免疫球蛋白M、补体3、补体4水平较高(P <；0.05)。屠宰性能和盲肠短链脂肪酸参数无显著差异(P >；PCE组与CON组间差异无统计学意义（0.05）。PCE的纳入显著增加(P <；0.05)，法氏囊/活重值与对照组相比显著降低(P <；0.05)增加了norank_f_Muribaculaceae、厌氧菌、Shuttleworthia、Brachyspira和Tuzzerella的相对丰度，但显著降低了(P <；0.05) unclassified_f_ rikenellaceae、Oscillospira、norank_f_ barnesiellaceae、norank_f_ christensenellaceae和Candidatus_Soleaferrea的相对丰度。共有2846个差异表达基因(deg；P & lt;0.05)，共鉴定出1140个上调基因和1706个下调基因。其中201个基因被注释到基因本体和京都基因与基因组百科全书免疫相关基因数据库中。蛋白-蛋白相互作用网络分析显示，与多聚体IgA和IgM连接链相关的DEGs是鸡免疫相关基因的显著上调，与叉头盒P1、抗菌肽1、抗菌肽2和抗菌肽3相关的DEGs是鸡免疫相关基因的显著下调。由此可见，饲粮中添加PCE有可能提高鸡的血浆免疫球蛋白、免疫器官、盲肠潜在有益菌水平和免疫相关基因表达，从而提高鸡的免疫功能。

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Effects of purple corn anthocyanin on slaughter performance, immune function, the caecal microbiota and the transcriptome in chickens

Poultry are susceptible to oxidative stress, which decreases immune function and negatively affects production performance under highly intensive feeding conditions. Moreover, anthocyanins can alleviate oxidative stress and improve immune functions in chickens. This study aimed to elucidate the effects of purple corn anthocyanin extract (PCE) on slaughter performance, immune function, the caecal microbiota and the transcriptome in chickens. A total of 180 female chickens were randomly divided into two groups, with one receiving a basal diet (CON) and one receiving a treatment (PCE) supplemented with 360 mg/kg PCE according to a completely randomized design. The results indicated that the levels of plasma immunoglobulin A, immunoglobulin G, immunoglobulin M, complement 3, and complement 4 in the PCE treatment group were greater (P < 0.05) than those in the CON group. The slaughter performance and caecal short-chain fatty acid parameters did not differ (P > 0.05) between the PCE and CON groups. The inclusion of PCE significantly increased (P < 0.05) the bursa of Fabricius/live weight value compared with those of the CON. Chickens receiving PCE had significantly (P < 0.05) increased relative abundances of norank_f_Muribaculaceae, Anaerofilum, Shuttleworthia, Brachyspira, and Tuzzerella but significantly decreased (P < 0.05) relative abundances of unclassified_f__Rikenellaceae, Oscillospira, norank_f__Barnesiellaceae, norank_f__Christensenellaceae, and Candidatus_Soleaferrea. A total of 2,846 differentially expressed genes (DEGs; P < 0.05), which consisted of 1,140 upregulated genes and 1,706 downregulated genes, were identified. Among them, 201 genes were annotated to the Gene Ontology and Kyoto Encyclopedia of Genes and Genomes database for immune-related genes. Protein-protein interaction network analysis revealed that DEGs associated with the joining chain of multimeric IgA and IgM were significantly upregulated immune-related genes, and those associated with forkhead box P1, cathelicidin 1, cathelicidin 2, and cathelicidin 3 were significantly downregulated immune-related genes in chickens. The findings demonstrated that dietary supplementation with PCE has the potential to improve plasma immunoglobulin, immune organ, caecal potentially beneficial bacteria levels and immune-related gene expressions, which can increase the immune function of chickens.

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来源期刊

Poultry Science 农林科学-奶制品与动物科学

CiteScore

7.60

自引率

15.90%

发文量

审稿时长

94 days

期刊介绍： First self-published in 1921, Poultry Science is an internationally renowned monthly journal, known as the authoritative source for a broad range of poultry information and high-caliber research. The journal plays a pivotal role in the dissemination of preeminent poultry-related knowledge across all disciplines. As of January 2020, Poultry Science will become an Open Access journal with no subscription charges, meaning authors who publish here can make their research immediately, permanently, and freely accessible worldwide while retaining copyright to their work. Papers submitted for publication after October 1, 2019 will be published as Open Access papers. An international journal, Poultry Science publishes original papers, research notes, symposium papers, and reviews of basic science as applied to poultry. This authoritative source of poultry information is consistently ranked by ISI Impact Factor as one of the top 10 agriculture, dairy and animal science journals to deliver high-caliber research. Currently it is the highest-ranked (by Impact Factor and Eigenfactor) journal dedicated to publishing poultry research. Subject areas include breeding, genetics, education, production, management, environment, health, behavior, welfare, immunology, molecular biology, metabolism, nutrition, physiology, reproduction, processing, and products.