柿叶提取物通过调节高脂饮食小鼠的脂质基因表达和肠道微生物群来改善高脂血症。

IF 3.3 2区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY

Journal of the Science of Food and Agriculture Pub Date : 2025-03-31 DOI:10.1002/jsfa.14248

Yuanyuan Gao, Jing Liu, Xiaojuan Liu, Yuexin Hao, Zhaofeng Pan, Xiaohua He, Bin Liu, Xuchang Duan

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引用次数: 0

摘要

背景：高脂血症以血脂异常升高为特征。柿叶具有多种药理活性，具有降脂作用。然而，关于柿叶降血脂作用机制的报道很少。采用高分辨率液相色谱-电喷雾-串联质谱联用技术和高效液相色谱法对柿叶乙醇提取物的成分进行了鉴定。基于脂质基因表达和肠道菌群，探讨PLE抗高脂饮食小鼠高脂血症的机制。结果：从荷叶提取物中鉴定出27个降血脂成分，其中主要降血脂成分为黄芪甲苷、金丝桃苷、儿茶素、绿原酸和槲皮素。补充PLE可降低血脂，减少肝损伤、脂质积累和炎症。脂质基因表达分析表明，PLE下调脂质合成基因FAS（脂肪酸合成酶）的表达；结论：PLE可预防高脂血症大鼠脂质代谢紊乱，调节肠道菌群稳态。这项研究为PLE作为一种预防高脂血症的天然活性物质提供了见解。©2025化学工业协会。

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Persimmon leaf extract ameliorates hyperlipidemia by modulating lipid genes expression and gut microbiota in high-fat-diet-fed mice.

Background: Hyperlipidemia is characterized by abnormally elevated blood lipids. Persimmon leaf has multiple pharmacological activities and is valued for its lipid-lowering effect. However, few reports have revealed the hypolipidemic mechanism of persimmon leaf. In this study, compositions from the ethanol extract of persimmon leaf (PLE) were identified by high-resolution liquid chromatography-electrospray ionization-tandem mass spectrometry and high-performance liquid chromatography. The mechanism of PLE against hyperlipidemia induced by high-fat diet in mice was then explored based on lipid gene expression and gut microbiota.

Results: The study demonstrated that 27 compositions from PLE were identified, of which the primary hypolipidemic compositions were astragalin, hyperoside, catechin, chlorogenic acid, and quercetin. Supplementation of PLE could reduce serum lipids, liver injury, lipid accumulation, and inflammation. The analysis of lipid gene expression indicated that PLE downregulated the expression of lipid synthesis genes FAS (fatty acid synthase; P < 0.001), ACC (acetyl coenzyme A carboxylase; P < 0.01), SCD1 (stearyl coenzyme A dehydrogenase 1; P < 0.05) and SREBP-1c (sterol regulatory element binding protein 1c; P < 0.01), while upregulating the expression of lipid degradation genes PPAR-α (peroxide-activated receptor alpha; P < 0.05) and CYP7A1 (cholesterol 7α-hydroxylase; P < 0.05). Simultaneously, PLE greatly recovered the intestinal short-chain fatty acid content, especially butyric acid (P < 0.05), valeric acid (P < 0.01) and isovaleric acid (P < 0.05). Furthermore, 16S rRNA analysis showed that PLE decreased the Firmicutes/Bacteroidetes ratio and increased the abundance of Lactobacillus, Turicibacter, and Dubosiella microbiota, which maintained the homeostasis of intestinal flora.

Conclusion: PLE could prevent lipid metabolism disorders and modulate gut microbiota homeostasis in hyperlipidemic rats. This study provides insights into PLE as a natural active substance for the prevention of hyperlipidemia. © 2025 Society of Chemical Industry.

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来源期刊

Journal of the Science of Food and Agriculture 工程技术-农业综合

CiteScore

8.10

自引率

4.90%

发文量

634

审稿时长

3.1 months

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