Jin-jin Wang, Xin-yan Zhang, Mei-fang Hua, Si-lun Li, Jie Wei, Qing-min Chen, Tian-hui Jiao, Yi Xu, Min Chen and Xiao-mei Chen*,
{"title":"空间分辨电化学发光生物传感器联合纳米孔筛选装置同时测定脱氧雪腐菌烯醇和黄曲霉毒素B1","authors":"Jin-jin Wang, Xin-yan Zhang, Mei-fang Hua, Si-lun Li, Jie Wei, Qing-min Chen, Tian-hui Jiao, Yi Xu, Min Chen and Xiao-mei Chen*, ","doi":"10.1021/acs.analchem.5c0033610.1021/acs.analchem.5c00336","DOIUrl":null,"url":null,"abstract":"<p >Improving the preprocessing efficiency and creating multiple detection channels are crucial for building high-performance sensors. In this study, we developed a sensing platform that integrates a spatially resolved electrochemiluminescence (ECL) biosensor with a nanopore screening device for the simultaneous detection of deoxynivalenol (DON) and aflatoxin B<sub>1</sub> (AFB<sub>1</sub>) in wheat. Zeolitic imidazolate framework-67(ZIF-67), which promotes the production of oxygen radicals (O<sub>2</sub>̇<sup>–</sup>), was integrated with luminol-capped silver nanoparticles (Luminol-AgNPs) to function as an ECL probe for DON detection. CON<sub>4</sub>H<sub>6</sub>–Ru, synthesized by covalently linking tris(4,4′-dicarboxylicacid-2,2′-bipyridyl) ruthenium(II) dichloride [Ru(dcbpy)<sub>3</sub>Cl<sub>2</sub>] and carbonyldiazide (CON<sub>4</sub>H<sub>6</sub>), was encapsulated in mesoporous silica nanoparticles (MSNs) and used as another ECL probe for the detection of AFB<sub>1</sub>. Subsequently, a reusable nanopore screening device was designed to eliminate macromolecular interference in the samples and improve the interference resistance of the sensing platform. Under the optimal experimental conditions, the sensing platform showed good linearity for DON concentrations from 0.005 to 150 μg/kg and AFB<sub>1</sub> concentrations from 0.05 to 100 μg/kg, with detection limits of 1.80 × 10<sup>–4</sup> and 1.09 × 10<sup>–3</sup> μg/kg (S/N = 3), respectively.</p>","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"97 12","pages":"6788–6795 6788–6795"},"PeriodicalIF":6.7000,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Spatially Resolved Electrochemiluminescent Biosensor Combined with Nanopore Screening Devices for Simultaneous Determination of Deoxynivalenol and Aflatoxin B1\",\"authors\":\"Jin-jin Wang, Xin-yan Zhang, Mei-fang Hua, Si-lun Li, Jie Wei, Qing-min Chen, Tian-hui Jiao, Yi Xu, Min Chen and Xiao-mei Chen*, \",\"doi\":\"10.1021/acs.analchem.5c0033610.1021/acs.analchem.5c00336\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p >Improving the preprocessing efficiency and creating multiple detection channels are crucial for building high-performance sensors. In this study, we developed a sensing platform that integrates a spatially resolved electrochemiluminescence (ECL) biosensor with a nanopore screening device for the simultaneous detection of deoxynivalenol (DON) and aflatoxin B<sub>1</sub> (AFB<sub>1</sub>) in wheat. Zeolitic imidazolate framework-67(ZIF-67), which promotes the production of oxygen radicals (O<sub>2</sub>̇<sup>–</sup>), was integrated with luminol-capped silver nanoparticles (Luminol-AgNPs) to function as an ECL probe for DON detection. CON<sub>4</sub>H<sub>6</sub>–Ru, synthesized by covalently linking tris(4,4′-dicarboxylicacid-2,2′-bipyridyl) ruthenium(II) dichloride [Ru(dcbpy)<sub>3</sub>Cl<sub>2</sub>] and carbonyldiazide (CON<sub>4</sub>H<sub>6</sub>), was encapsulated in mesoporous silica nanoparticles (MSNs) and used as another ECL probe for the detection of AFB<sub>1</sub>. Subsequently, a reusable nanopore screening device was designed to eliminate macromolecular interference in the samples and improve the interference resistance of the sensing platform. Under the optimal experimental conditions, the sensing platform showed good linearity for DON concentrations from 0.005 to 150 μg/kg and AFB<sub>1</sub> concentrations from 0.05 to 100 μg/kg, with detection limits of 1.80 × 10<sup>–4</sup> and 1.09 × 10<sup>–3</sup> μg/kg (S/N = 3), respectively.</p>\",\"PeriodicalId\":27,\"journal\":{\"name\":\"Analytical Chemistry\",\"volume\":\"97 12\",\"pages\":\"6788–6795 6788–6795\"},\"PeriodicalIF\":6.7000,\"publicationDate\":\"2025-03-21\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Analytical Chemistry\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://pubs.acs.org/doi/10.1021/acs.analchem.5c00336\",\"RegionNum\":1,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"CHEMISTRY, ANALYTICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Analytical Chemistry","FirstCategoryId":"92","ListUrlMain":"https://pubs.acs.org/doi/10.1021/acs.analchem.5c00336","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
Spatially Resolved Electrochemiluminescent Biosensor Combined with Nanopore Screening Devices for Simultaneous Determination of Deoxynivalenol and Aflatoxin B1
Improving the preprocessing efficiency and creating multiple detection channels are crucial for building high-performance sensors. In this study, we developed a sensing platform that integrates a spatially resolved electrochemiluminescence (ECL) biosensor with a nanopore screening device for the simultaneous detection of deoxynivalenol (DON) and aflatoxin B1 (AFB1) in wheat. Zeolitic imidazolate framework-67(ZIF-67), which promotes the production of oxygen radicals (O2̇–), was integrated with luminol-capped silver nanoparticles (Luminol-AgNPs) to function as an ECL probe for DON detection. CON4H6–Ru, synthesized by covalently linking tris(4,4′-dicarboxylicacid-2,2′-bipyridyl) ruthenium(II) dichloride [Ru(dcbpy)3Cl2] and carbonyldiazide (CON4H6), was encapsulated in mesoporous silica nanoparticles (MSNs) and used as another ECL probe for the detection of AFB1. Subsequently, a reusable nanopore screening device was designed to eliminate macromolecular interference in the samples and improve the interference resistance of the sensing platform. Under the optimal experimental conditions, the sensing platform showed good linearity for DON concentrations from 0.005 to 150 μg/kg and AFB1 concentrations from 0.05 to 100 μg/kg, with detection limits of 1.80 × 10–4 and 1.09 × 10–3 μg/kg (S/N = 3), respectively.
期刊介绍：
Analytical Chemistry, a peer-reviewed research journal, focuses on disseminating new and original knowledge across all branches of analytical chemistry. Fundamental articles may explore general principles of chemical measurement science and need not directly address existing or potential analytical methodology. They can be entirely theoretical or report experimental results. Contributions may cover various phases of analytical operations, including sampling, bioanalysis, electrochemistry, mass spectrometry, microscale and nanoscale systems, environmental analysis, separations, spectroscopy, chemical reactions and selectivity, instrumentation, imaging, surface analysis, and data processing. Papers discussing known analytical methods should present a significant, original application of the method, a notable improvement, or results on an important analyte.
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