Circulating progesterone concentrations and pregnancy outcomes in high-producing lactating dairy cows treated with human chorionic gonadotropin on day 2 of the estrus cycle

Suboptimal concentrations of circulating progesterone (P4) in the early postovulatory period have been associated with low fertility observed in high-producing lactating dairy cows. The administration of human chorionic gonadotropin (hCG) increases P4 in cattle by stimulating its endogenous production by the corpus luteum (CL) and creating an accessory CL if administered at an appropriate stage of the cycle. The aim of this study was to evaluate the effect of a single administration of hCG on d 2 of the estrus cycle on circulating P4 concentrations and pregnancy per artificial insemination (P/AI) in high-producing lactating dairy cows in confinement systems. To that end, 796 lactating Holstein-Friesian dairy cows from 15 farms were enrolled on this study. The mean ± SD parity and DIM at enrollment were 2.3 ± 1.4 and 86.7 ± 17.8, respectively. After a voluntary waiting period (50–60 d after calving), cows underwent fixed-time artificial insemination (FTAI) following a routine protocol for synchronization of ovulation (Double Ovsynch, G6G, or 7-d Ovsynch + P4). Cows on each farm were blocked on parity, DIM, BCS, and synchronization protocol and were randomly assigned to receive either 3,000 IU of hCG (n = 420) or an equivalent volume of saline solution (control; n = 376) on d 2 after estimated estrus (∼16 h before FTAI = d 0). Blood samples were collected from a subset of cows from both treatments (control n = 65 and hCG n = 65) on d 0, 7, and 14 of the estrus cycle to measure serum P4 concentrations. Pregnancy per artificial insemination on d 30 after FTAI was affected by hCG treatment and parity. Moreover, there was a tendency for an interaction between treatment and parity. Overall, treatment with hCG on d 2 of the estrus cycle increased P/AI (45.2% vs. control 38.8%). In first- or second-lactation cows, P/AI was similar between hCG-treated and control cows (47.1% vs. control 45.9%). Conversely, cows in third or greater lactation treated with hCG had greater P/AI (42.1% vs. control 27.3%). The overall incidence of pregnancy loss between d 30 and d 70 was 14.7% and was not affected by treatment. Cows treated with hCG had higher P4 concentrations on d 7 and 14 compared with control cows (3.4 ± 0.66 vs. 3.0 ± 0.58 ng/mL and 6.6 ± 1.28 vs. 5.3 ± 1.02 ng/mL, respectively). Moreover, an interaction between treatment and parity revealed that P4 concentrations were higher in hCG-treated cows in third or greater lactation compared with control cows in the same parity group on d 7 and 14, while no differences were observed in first- or second-lactation cows. In conclusion, administration of hCG on d 2 of the estrus cycle increased fertility in high-producing dairy cows in third or greater lactation, in association with an early increase of circulating P4.