小麦串联激酶激活NLR触发免疫

IF 45.8 1区综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES

Science Pub Date : 2025-03-27 DOI:10.1126/science.adp5034

Renjie Chen, Jian Chen, Oliver R. Powell, Megan A. Outram, Taj Arndell, Karthick Gajendiran, Yan L. Wang, Jibril Lubega, Yang Xu, Michael A. Ayliffe, Cheryl Blundell, Melania Figueroa, Jana Sperschneider, Thomas Vanhercke, Kostya Kanyuka, Dingzhong Tang, Guitao Zhong, Catherine Gardener, Guotai Yu, Spyridon Gourdoupis, Łukasz Jaremko, Oadi Matny, Brian J. Steffenson, Willem H. P. Boshoff, Wilku B. Meyer, Stefan T. Arold, Peter N. Dodds, Brande B. H. Wulff

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引用次数: 0

摘要

核苷酸结合的富亮氨酸重复（NLR）受体在植物免疫中的作用已被深入研究，但一类串联激酶（TK）在小麦和大麦中赋予抗病性的功能仍不清楚。在本研究中，我们发现 SR62 基因座是一个编码 Sr62 TK TK 和一个 NLR（Sr62 NLR）的二基因模块，并确定了相应的 AvrSr62 效应子。AvrSr62 与 Sr62 TK 的 N 端激酶 1 结合，引发激酶 2 位移，从而激活 Sr62 NLR。建模和突变分析表明，这是通过重叠的结合位点介导的：(i) 结合 AvrSr62 和激酶 2 的激酶 1；(ii) 结合激酶 1 和 Sr62 NLR 的激酶 2。了解这种双组分抗性复合体可能有助于工程设计和培育具有持久抗性的植物。

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A wheat tandem kinase activates an NLR to trigger immunity

The role of nucleotide-binding leucine-rich repeat (NLR) receptors in plant immunity is well studied, but the function of a class of tandem kinases (TKs) that confer disease resistance in wheat and barley remains unclear. In this study, we show that the SR62 locus is a digenic module encoding the Sr62^TK TK and an NLR (Sr62^NLR), and we identify the corresponding AvrSr62 effector. AvrSr62 binds to the N-terminal kinase 1 of Sr62^TK, triggering displacement of kinase 2, which activates Sr62^NLR. Modeling and mutation analysis indicated that this is mediated by overlapping binding sites (i) on kinase 1 for binding AvrSr62 and kinase 2 and (ii) on kinase 2 for binding kinase 1 and Sr62^NLR. Understanding this two-component resistance complex may help engineering and breeding plants for durable resistance.

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来源期刊

Science 综合性期刊-综合性期刊

CiteScore

61.10

自引率

0.90%

发文量

审稿时长

2.1 months

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