红外线和电刺激下神经纤维传导速度的差异。

IF 2.7 4区医学 Q2 BIOCHEMICAL RESEARCH METHODS

Journal of Neuroscience Methods Pub Date : 2025-03-16 DOI:10.1016/j.jneumeth.2025.110427

Louis Vande Perre , Javier Chávez Cerda , Soizic Gochard , Maxime Verstraeten , Romain Raffoul , Catherine Leonard , Jean Delbeke , Riëm El Tahry , Simon-Pierre Gorza , Antoine Nonclercq

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引用次数: 0

摘要

背景：红外神经刺激（INS）利用短光脉冲来激活神经。虽然电刺激（ES）首先激活大直径纤维，但光可能优先激活小直径纤维，这在许多临床应用中可能很有价值。新方法：本研究采用1470nm的紧凑二极管激光器进行惯性导联。通过传导速度（CV）测定大鼠坐骨神经和山羊迷走神经INS和ES纤维类型激活的差异。选择大鼠坐骨神经作为标准模型，是因为它的生理特性很好，在INS机制的研究中有广泛的应用。选择山羊迷走神经是因为其预期的高比例的小直径纤维和较大的尺寸，这使得记录单元之间有足够的分离，以优化CNAP测量。结果：在大鼠坐骨神经中，es兴奋纤维的CVs值（9.81±3.18m/s）显著高于ins兴奋纤维的CVs值（8.10±2.82m/s）。在山羊迷走神经中，ES产生的平均CV为6.47±1.25m/s，而INS没有产生明显可区分的复合神经动作电位，突出了INS应用于更大神经的挑战。与现有方法的比较：据我们所知，我们首次通过测量CV来识别受INS刺激的神经纤维类型。结论：这些结果表明INS可能优先激活较小直径的纤维，为潜在的神经调节应用提供了见解。

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Differences in conduction velocities of nerve fibers excited by infrared and electrical stimulation

Background

Infrared neural stimulation (INS) uses short optical pulses to activate nerves. While electrical stimulation (ES) activates large-diameter fibers first, light may preferentially activate small-diameter fibers first, which could be valuable for many clinical applications.

New method

This study used a compact diode laser of 1470 nm to perform INS. Conduction velocity (CV) measurements were performed to assess differences in fiber type activation between INS and ES in the rat sciatic nerve and the goat vagus nerve. The rat sciatic nerve was chosen as a standard model because of its well-characterized physiology and extensive use in studies of INS mechanisms. The goat vagus nerve was chosen because of its expected high proportion of small-diameter fibers and its larger size, which allows sufficient separation between recording units to optimize CNAP measurements.

Results

The results showed that in the rat sciatic nerve, ES-excited fibers had significantly higher CVs (9.81 ± 3.18 m/s) than INS-excited fibers (8.10 ± 2.82 m/s). In the goat vagus nerve, ES produced a mean CV of 6.47 ± 1.25 m/s, but INS did not produce clearly distinguishable compound nerve action potential, highlighting the challenges of applying INS to larger nerves.

Comparison to existing methods

To the best of our knowledge, CV is, for the first time, measured to identify the type of nerve fiber excited by INS.

Conclusion

These results suggest that INS may preferentially activate smaller diameter fibers, providing insight for potential neuromodulation applications.

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来源期刊

Journal of Neuroscience Methods 医学-神经科学

CiteScore

7.10

自引率

3.30%

发文量

226

审稿时长

52 days

期刊介绍： The Journal of Neuroscience Methods publishes papers that describe new methods that are specifically for neuroscience research conducted in invertebrates, vertebrates or in man. Major methodological improvements or important refinements of established neuroscience methods are also considered for publication. The Journal''s Scope includes all aspects of contemporary neuroscience research, including anatomical, behavioural, biochemical, cellular, computational, molecular, invasive and non-invasive imaging, optogenetic, and physiological research investigations.