Jun-ming Zhang, Yu-jie Luo, Zhi-yi Xu, Shan-shan Chen, Zhen-peng Kai
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Specifically, the insecticidal activity of nano-Hv1a was maintained at 37.19 ± 1.23%, in stark contrast to merely 1.60 ± 0.63% for Hv1a after 24 h under conditions conducive to photodegradation. Additionally, the encapsulated form demonstrated a prolonged retention time within insects, resulting in enhanced insecticidal efficacy compared to non-encapsulated Hv1a alone. The study further explored the effects of nano-Hv1a on insect growth patterns, revealing marked weight loss and sustained insecticidal activity beyond that observed with plain Hv1a.</p><h3>Conclusion</h3><p>The findings underscore the potential of nano-Hv1a as an efficient and environmentally sustainable alternative for pest control strategies in agriculture. By improving both stability and effectiveness through advanced encapsulation techniques, this research proposes a viable complement to conventional pesticide practices.</p><h3>Graphical Abstract</h3>\n<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":512,"journal":{"name":"Chemical and Biological Technologies in Agriculture","volume":"12 1","pages":""},"PeriodicalIF":5.2000,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://chembioagro.springeropen.com/counter/pdf/10.1186/s40538-025-00756-4","citationCount":"0","resultStr":"{\"title\":\"Utilization of nanotechnology to improve the stability and insecticidal activity of spider venom protein Hv1a\",\"authors\":\"Jun-ming Zhang, Yu-jie Luo, Zhi-yi Xu, Shan-shan Chen, Zhen-peng Kai\",\"doi\":\"10.1186/s40538-025-00756-4\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Background</h3><p>With the advancement of nanotechnology, nano biopesticides have gained considerable attention. This study presents a novel method for enhancing the stability and efficacy of the spider venom protein ω-HXTX-Hv1a, an insecticide neurotoxin. The approach involves encapsulating this protein within chitosan cross-linked sodium tripolyphosphate nanocapsules. Such encapsulation is designed to improve the adhesion of Hv1a to leaf surfaces while protecting it from degradation induced by ultraviolet light.</p><h3>Results</h3><p>Encapsulation of Hv1a in nanocapsules yielded significant advantages. Specifically, the insecticidal activity of nano-Hv1a was maintained at 37.19 ± 1.23%, in stark contrast to merely 1.60 ± 0.63% for Hv1a after 24 h under conditions conducive to photodegradation. Additionally, the encapsulated form demonstrated a prolonged retention time within insects, resulting in enhanced insecticidal efficacy compared to non-encapsulated Hv1a alone. The study further explored the effects of nano-Hv1a on insect growth patterns, revealing marked weight loss and sustained insecticidal activity beyond that observed with plain Hv1a.</p><h3>Conclusion</h3><p>The findings underscore the potential of nano-Hv1a as an efficient and environmentally sustainable alternative for pest control strategies in agriculture. 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Utilization of nanotechnology to improve the stability and insecticidal activity of spider venom protein Hv1a
Background
With the advancement of nanotechnology, nano biopesticides have gained considerable attention. This study presents a novel method for enhancing the stability and efficacy of the spider venom protein ω-HXTX-Hv1a, an insecticide neurotoxin. The approach involves encapsulating this protein within chitosan cross-linked sodium tripolyphosphate nanocapsules. Such encapsulation is designed to improve the adhesion of Hv1a to leaf surfaces while protecting it from degradation induced by ultraviolet light.
Results
Encapsulation of Hv1a in nanocapsules yielded significant advantages. Specifically, the insecticidal activity of nano-Hv1a was maintained at 37.19 ± 1.23%, in stark contrast to merely 1.60 ± 0.63% for Hv1a after 24 h under conditions conducive to photodegradation. Additionally, the encapsulated form demonstrated a prolonged retention time within insects, resulting in enhanced insecticidal efficacy compared to non-encapsulated Hv1a alone. The study further explored the effects of nano-Hv1a on insect growth patterns, revealing marked weight loss and sustained insecticidal activity beyond that observed with plain Hv1a.
Conclusion
The findings underscore the potential of nano-Hv1a as an efficient and environmentally sustainable alternative for pest control strategies in agriculture. By improving both stability and effectiveness through advanced encapsulation techniques, this research proposes a viable complement to conventional pesticide practices.
期刊介绍：
Chemical and Biological Technologies in Agriculture is an international, interdisciplinary, peer-reviewed forum for the advancement and application to all fields of agriculture of modern chemical, biochemical and molecular technologies. The scope of this journal includes chemical and biochemical processes aimed to increase sustainable agricultural and food production, the evaluation of quality and origin of raw primary products and their transformation into foods and chemicals, as well as environmental monitoring and remediation. Of special interest are the effects of chemical and biochemical technologies, also at the nano and supramolecular scale, on the relationships between soil, plants, microorganisms and their environment, with the help of modern bioinformatics. Another special focus is the use of modern bioorganic and biological chemistry to develop new technologies for plant nutrition and bio-stimulation, advancement of biorefineries from biomasses, safe and traceable food products, carbon storage in soil and plants and restoration of contaminated soils to agriculture.
This journal presents the first opportunity to bring together researchers from a wide number of disciplines within the agricultural chemical and biological sciences, from both industry and academia. The principle aim of Chemical and Biological Technologies in Agriculture is to allow the exchange of the most advanced chemical and biochemical knowledge to develop technologies which address one of the most pressing challenges of our times - sustaining a growing world population.
Chemical and Biological Technologies in Agriculture publishes original research articles, short letters and invited reviews. Articles from scientists in industry, academia as well as private research institutes, non-governmental and environmental organizations are encouraged.
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