Guangxue Zhu, Yuanyuan Yue, Rongjia Zhou, Bingxue Sun, Xuewen Xie, Ali Chai, Lei Li, Tengfei Fan, Baoju Li* and Yanxia Shi*,
{"title":"黄瓜对Boscalid和Trifloxystrobin的敏感性及与CcSdh和CcCytb相关的cassiicola适应度","authors":"Guangxue Zhu, Yuanyuan Yue, Rongjia Zhou, Bingxue Sun, Xuewen Xie, Ali Chai, Lei Li, Tengfei Fan, Baoju Li* and Yanxia Shi*, ","doi":"10.1021/acs.jafc.4c1243110.1021/acs.jafc.4c12431","DOIUrl":null,"url":null,"abstract":"<p >The extensive use of succinate dehydrogenase inhibitors (SDHIs) and quinone outside inhibitors (QoIs) has led to the development of resistance in <i>Corynespora cassiicola</i>, which causes corynespora leaf spot (CLS) in cucumber. In this study, the sensitivity and the resistance phenotypes to SDHIs and QoIs were monitored, and the fitness of <i>C. cassiicola</i> carrying <i>CcSdh</i> & <i>CcCytb</i> mutations was measured. The average 50% effective suppressive concentration (EC<sub>50</sub>) values of <i>C. cassiicola</i> to boscalid from 2019 to 2021 ranged from 6.65 to 24.16 μg/mL, while the average EC<sub>50</sub> values of <i>C. cassiicola</i> to trifloxystrobin from 2007 to 2021 ranged from 105.11 to 462.02 μg/mL. Ten fungicide resistance phenotypes and 19 resistance genotypes were found. The D-D95E&Cytb-G143A and B–H278L&Cytb-G143A mutants presented increased fitness, while the B–H278Y&Cytb-G143A mutant showed significant fitness costs, and the other mutants presented no or slight fitness costs. These findings provide a basis for the formulation of resistance management strategies of CLS.</p>","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"73 11","pages":"6545–6558 6545–6558"},"PeriodicalIF":6.2000,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Sensitivity to Boscalid and Trifloxystrobin and Fitness of Corynespora cassiicola Associated with CcSdh & CcCytb in Cucumber\",\"authors\":\"Guangxue Zhu, Yuanyuan Yue, Rongjia Zhou, Bingxue Sun, Xuewen Xie, Ali Chai, Lei Li, Tengfei Fan, Baoju Li* and Yanxia Shi*, \",\"doi\":\"10.1021/acs.jafc.4c1243110.1021/acs.jafc.4c12431\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p >The extensive use of succinate dehydrogenase inhibitors (SDHIs) and quinone outside inhibitors (QoIs) has led to the development of resistance in <i>Corynespora cassiicola</i>, which causes corynespora leaf spot (CLS) in cucumber. In this study, the sensitivity and the resistance phenotypes to SDHIs and QoIs were monitored, and the fitness of <i>C. cassiicola</i> carrying <i>CcSdh</i> & <i>CcCytb</i> mutations was measured. The average 50% effective suppressive concentration (EC<sub>50</sub>) values of <i>C. cassiicola</i> to boscalid from 2019 to 2021 ranged from 6.65 to 24.16 μg/mL, while the average EC<sub>50</sub> values of <i>C. cassiicola</i> to trifloxystrobin from 2007 to 2021 ranged from 105.11 to 462.02 μg/mL. Ten fungicide resistance phenotypes and 19 resistance genotypes were found. The D-D95E&Cytb-G143A and B–H278L&Cytb-G143A mutants presented increased fitness, while the B–H278Y&Cytb-G143A mutant showed significant fitness costs, and the other mutants presented no or slight fitness costs. These findings provide a basis for the formulation of resistance management strategies of CLS.</p>\",\"PeriodicalId\":41,\"journal\":{\"name\":\"Journal of Agricultural and Food Chemistry\",\"volume\":\"73 11\",\"pages\":\"6545–6558 6545–6558\"},\"PeriodicalIF\":6.2000,\"publicationDate\":\"2025-03-10\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Agricultural and Food Chemistry\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://pubs.acs.org/doi/10.1021/acs.jafc.4c12431\",\"RegionNum\":1,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"AGRICULTURE, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Agricultural and Food Chemistry","FirstCategoryId":"97","ListUrlMain":"https://pubs.acs.org/doi/10.1021/acs.jafc.4c12431","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRICULTURE, MULTIDISCIPLINARY","Score":null,"Total":0}
Sensitivity to Boscalid and Trifloxystrobin and Fitness of Corynespora cassiicola Associated with CcSdh & CcCytb in Cucumber
The extensive use of succinate dehydrogenase inhibitors (SDHIs) and quinone outside inhibitors (QoIs) has led to the development of resistance in Corynespora cassiicola, which causes corynespora leaf spot (CLS) in cucumber. In this study, the sensitivity and the resistance phenotypes to SDHIs and QoIs were monitored, and the fitness of C. cassiicola carrying CcSdh & CcCytb mutations was measured. The average 50% effective suppressive concentration (EC50) values of C. cassiicola to boscalid from 2019 to 2021 ranged from 6.65 to 24.16 μg/mL, while the average EC50 values of C. cassiicola to trifloxystrobin from 2007 to 2021 ranged from 105.11 to 462.02 μg/mL. Ten fungicide resistance phenotypes and 19 resistance genotypes were found. The D-D95E&Cytb-G143A and B–H278L&Cytb-G143A mutants presented increased fitness, while the B–H278Y&Cytb-G143A mutant showed significant fitness costs, and the other mutants presented no or slight fitness costs. These findings provide a basis for the formulation of resistance management strategies of CLS.
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The Journal of Agricultural and Food Chemistry publishes high-quality, cutting edge original research representing complete studies and research advances dealing with the chemistry and biochemistry of agriculture and food. The Journal also encourages papers with chemistry and/or biochemistry as a major component combined with biological/sensory/nutritional/toxicological evaluation related to agriculture and/or food.
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