{"title":"酶解™液体d -葡萄糖/ d -果糖用于选定食品和饮料中d -葡萄糖和d -果糖的酶测定的验证：第一次行动2024.04。","authors":"Markus Lacorn, Thomas Hektor","doi":"10.1093/jaoacint/qsaf019","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>D-Glucose and D-fructose are present in honey, wine and beer, and in a range of other foodstuffs such as bread, pastries or chocolate. Both sugars can occur as a monosaccharide or in di-, oligo- and polysaccharides.</p><p><strong>Objective: </strong>To validate the performance of the Enzytec™ Liquid D-Glucose/D-Fructose test kit for the determination of D-glucose and D-fructose in food and beverages such as fruit and vegetable juices, soft drinks, wines, and beer.</p><p><strong>Methods: </strong>The method is based on enzymes which are part of a prepackaged kit that contains 3 ready-to-use components. Both sugars are phosphorylated by a hexokinase. Glucose-6-phosphate (G-6-P) and NAD are converted to gluconate-6-phosphate and NADH. NADH is measured at 340 nm. Phosphoglucose isomerase converts fructose-6-phosphate to G-6-P which in turn is converted to gluconate-6-phosphate and NADH.</p><p><strong>Results: </strong>The test is specific to D-glucose and D-fructose and shows no side activities or interferences with the exception of mannose and sulphite that interfere at 5.1 and 1.25 g/L or more, respectively. The measurement range is from 6.1 to 2000 mg/L for D-glucose and 5.6 to 1000 mg/L for D-fructose (100 µL test volume). Trueness was evaluated using NIST SRM 3282 (cranberry juice) and one reference wine. The recoveries ranged from 101 to 102%. Spiking of wine, beer, soft drinks and juices resulted in recoveries between 93 and 105%. Intermediate precision is below 6% for concentrations at 25 mg/L and below 4% for higher concentrations. For automation, three applications with different test volumes and different measurement ranges were validated. Linearity is given from 5.0 up to 10000 mg/L for the sum of both sugars.</p><p><strong>Conclusions: </strong>The method is robust and accurate for manual and automated applications and was approved as AOAC Official Method of Analysis℠.</p><p><strong>Highlights: </strong>The ready-to-use components of the test kit have a shelf of at least 29 months.</p>","PeriodicalId":94064,"journal":{"name":"Journal of AOAC International","volume":" ","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Validation of Enzytec™ Liquid D-Glucose/D-Fructose for Enzymatic Determination of D-Glucose and D-Fructose in Selected Foods and Beverages: First Action 2024.04.\",\"authors\":\"Markus Lacorn, Thomas Hektor\",\"doi\":\"10.1093/jaoacint/qsaf019\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>D-Glucose and D-fructose are present in honey, wine and beer, and in a range of other foodstuffs such as bread, pastries or chocolate. Both sugars can occur as a monosaccharide or in di-, oligo- and polysaccharides.</p><p><strong>Objective: </strong>To validate the performance of the Enzytec™ Liquid D-Glucose/D-Fructose test kit for the determination of D-glucose and D-fructose in food and beverages such as fruit and vegetable juices, soft drinks, wines, and beer.</p><p><strong>Methods: </strong>The method is based on enzymes which are part of a prepackaged kit that contains 3 ready-to-use components. Both sugars are phosphorylated by a hexokinase. Glucose-6-phosphate (G-6-P) and NAD are converted to gluconate-6-phosphate and NADH. NADH is measured at 340 nm. Phosphoglucose isomerase converts fructose-6-phosphate to G-6-P which in turn is converted to gluconate-6-phosphate and NADH.</p><p><strong>Results: </strong>The test is specific to D-glucose and D-fructose and shows no side activities or interferences with the exception of mannose and sulphite that interfere at 5.1 and 1.25 g/L or more, respectively. The measurement range is from 6.1 to 2000 mg/L for D-glucose and 5.6 to 1000 mg/L for D-fructose (100 µL test volume). Trueness was evaluated using NIST SRM 3282 (cranberry juice) and one reference wine. The recoveries ranged from 101 to 102%. Spiking of wine, beer, soft drinks and juices resulted in recoveries between 93 and 105%. Intermediate precision is below 6% for concentrations at 25 mg/L and below 4% for higher concentrations. For automation, three applications with different test volumes and different measurement ranges were validated. Linearity is given from 5.0 up to 10000 mg/L for the sum of both sugars.</p><p><strong>Conclusions: </strong>The method is robust and accurate for manual and automated applications and was approved as AOAC Official Method of Analysis℠.</p><p><strong>Highlights: </strong>The ready-to-use components of the test kit have a shelf of at least 29 months.</p>\",\"PeriodicalId\":94064,\"journal\":{\"name\":\"Journal of AOAC International\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2025-03-11\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of AOAC International\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1093/jaoacint/qsaf019\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of AOAC International","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1093/jaoacint/qsaf019","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Validation of Enzytec™ Liquid D-Glucose/D-Fructose for Enzymatic Determination of D-Glucose and D-Fructose in Selected Foods and Beverages: First Action 2024.04.
Background: D-Glucose and D-fructose are present in honey, wine and beer, and in a range of other foodstuffs such as bread, pastries or chocolate. Both sugars can occur as a monosaccharide or in di-, oligo- and polysaccharides.
Objective: To validate the performance of the Enzytec™ Liquid D-Glucose/D-Fructose test kit for the determination of D-glucose and D-fructose in food and beverages such as fruit and vegetable juices, soft drinks, wines, and beer.
Methods: The method is based on enzymes which are part of a prepackaged kit that contains 3 ready-to-use components. Both sugars are phosphorylated by a hexokinase. Glucose-6-phosphate (G-6-P) and NAD are converted to gluconate-6-phosphate and NADH. NADH is measured at 340 nm. Phosphoglucose isomerase converts fructose-6-phosphate to G-6-P which in turn is converted to gluconate-6-phosphate and NADH.
Results: The test is specific to D-glucose and D-fructose and shows no side activities or interferences with the exception of mannose and sulphite that interfere at 5.1 and 1.25 g/L or more, respectively. The measurement range is from 6.1 to 2000 mg/L for D-glucose and 5.6 to 1000 mg/L for D-fructose (100 µL test volume). Trueness was evaluated using NIST SRM 3282 (cranberry juice) and one reference wine. The recoveries ranged from 101 to 102%. Spiking of wine, beer, soft drinks and juices resulted in recoveries between 93 and 105%. Intermediate precision is below 6% for concentrations at 25 mg/L and below 4% for higher concentrations. For automation, three applications with different test volumes and different measurement ranges were validated. Linearity is given from 5.0 up to 10000 mg/L for the sum of both sugars.
Conclusions: The method is robust and accurate for manual and automated applications and was approved as AOAC Official Method of Analysis℠.
Highlights: The ready-to-use components of the test kit have a shelf of at least 29 months.
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