趋势绿原子转移自由基聚合修饰乳酸菌：作为生物吸附剂解毒谷物中黄曲霉毒素B1的应用。

IF 3.5 2区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY

Journal of the Science of Food and Agriculture Pub Date : 2025-03-12 DOI:10.1002/jsfa.14216

Bingchen Wang, Yifan Wang, Lin Chen, Xiuyuan Zhang, Kuo He

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引用次数: 0

摘要

背景：为解决粮食中黄曲霉毒素B1 （AFB1）对健康的严重危害，本研究采用基于聚多巴胺的原子转移自由基聚合（p-ATRP）和细胞色素c催化的原子转移自由基聚合（c-ATRP）作为细胞相容性修饰技术，在活的植物乳杆菌（LAB）细胞表面涂覆温度敏感聚合物聚n -异丙基丙烯酰胺（PNIPAAm）。结果：合成了两种新型生物吸附剂。PNIPAAm作为“AFB1真空吸尘器”层的加入显著提高了LAB的吸附效率，并实现了温控解吸。与p-ATRP相比，C - atrp使用细胞色素C（来自LAB的膜蛋白）作为生物催化剂取代p-ATRP中必需的铜催化剂，消除了铜毒性和污染的风险。利用Lagergren伪二阶框架和Freundlich框架可以精确地模拟LAB@PNIPAAm对AFB1的特殊吸附能力，吸附容量达到74.88 ng mL-1。此外，LAB@PNIPAAm具有独特的温度响应特性，在22°C时吸附效率为78%，在温度升高至37°C时温度控制解吸率为69%。值得注意的是，c-ATRP技术还提高了LAB的抗应力能力。结论：这些发现为活菌细胞的表面工程和复杂环境中多种污染物的有效生物修复提供了新的见解。©2025化学工业协会。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Trending green atom transfer radical polymerization (ATRP)-modified lactic acid bacteria: application as a bioadsorbent to detoxicate aflatoxin B1 in grains

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Trending green atom transfer radical polymerization (ATRP)-modified lactic acid bacteria: application as a bioadsorbent to detoxicate aflatoxin B1 in grains

BACKGROUND

To address the severe health risks posed by aflatoxin B₁ (AFB₁) in grain, this study employed polydopamine-based atom transfer radical polymerization (p-ATRP) and cytochrome C-catalyzed atom transfer radical polymerization (c-ATRP) as cytocompatible modification techniques to coat the surface of living Lactobacillus plantarum (LAB) cells with the temperature-sensitive polymer poly(N-isopropylacrylamide) (PNIPAAm).

RESULTS

Two novel bioadsorbents were synthesized. The incorporation of PNIPAAm as an ‘AFB₁ vacuum cleaner’ layer significantly enhances LAB's adsorption efficiency and enables temperature-controlled desorption. Compared with p-ATRP, c-ATRP uses cytochrome C (from LAB's membrane protein) as a biocatalyst to replace the necessary copper catalyst in p-ATRP, eliminating the risk of copper toxicity and pollution. The exceptional AFB₁ adsorption capabilities of LAB@PNIPAAm can be precisely modeled using the Lagergren pseudo-second-order and Freundlich frameworks, with an adsorption capacity attaining 74.88 ng mL⁻¹. Furthermore, LAB@PNIPAAm displays unique temperature-responsive properties, achieving an adsorption efficiency of 78% at 22 °C and a temperature-controlled desorption rate of 69% upon temperature elevation to 37 °C. It is noteworthy that the c-ATRP technology also enhances the stress resistance of LAB.

CONCLUSION

These findings offer novel insights into the surface engineering of viable bacterial cells and the efficient bioremediation of multiple pollutants in complex environments. © 2025 Society of Chemical Industry.

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来源期刊

Journal of the Science of Food and Agriculture 工程技术-农业综合

CiteScore

8.10

自引率

4.90%

发文量

634

审稿时长

3.1 months

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