{"title":"微滴介导的土壤过氧化物酶电位感应酶活性增强研究","authors":"Yinhao Li, Jiawang Ding, Wei Qin","doi":"10.1021/acs.analchem.5c00327","DOIUrl":null,"url":null,"abstract":"On-site measurements of enzyme activity in complex solid matrices such as soil and sediment could offer invaluable insights for the soil health evaluation and sustainable management. As such, sensors for on-site measurements of soil enzyme activity are highly required but remain unachieved to date. Herein, a potentiometric sensor for rapid, direct, and in-field analysis of soil enzyme activity is proposed, in which soil particle separation and soil enzyme extraction can be achieved within a single device. More importantly, the enzyme activity mediated by microdroplets can be greatly enhanced for potentiometric measurements. By using a classic soil redox enzyme-peroxidase and an anti-interference substrate 3,3′,5,5′-tetramethylbenzidine as a model, the potentiometric sensor enables directly measuring peroxidase activity in the field with a detection limit that can reach as low as 10<sup>–7</sup> U mL<sup>–1</sup> within 5 min. Moreover, the potentiometric sensor was deployed for on-site analysis of the peroxidase activity of <i>Suaeda salsa</i> (L.) Pall. rhizosphere soil under salinity stress, showing excellent sensitivity and anti-interference capability, and retaining promising potential for evaluating the mechanisms and feedback in response to environment stresses.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"16 1","pages":""},"PeriodicalIF":6.7000,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Microdroplet-Mediated Enzyme Activity Enhancement for Potentiometric Sensing of Peroxidase in Soil\",\"authors\":\"Yinhao Li, Jiawang Ding, Wei Qin\",\"doi\":\"10.1021/acs.analchem.5c00327\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"On-site measurements of enzyme activity in complex solid matrices such as soil and sediment could offer invaluable insights for the soil health evaluation and sustainable management. As such, sensors for on-site measurements of soil enzyme activity are highly required but remain unachieved to date. Herein, a potentiometric sensor for rapid, direct, and in-field analysis of soil enzyme activity is proposed, in which soil particle separation and soil enzyme extraction can be achieved within a single device. More importantly, the enzyme activity mediated by microdroplets can be greatly enhanced for potentiometric measurements. By using a classic soil redox enzyme-peroxidase and an anti-interference substrate 3,3′,5,5′-tetramethylbenzidine as a model, the potentiometric sensor enables directly measuring peroxidase activity in the field with a detection limit that can reach as low as 10<sup>–7</sup> U mL<sup>–1</sup> within 5 min. Moreover, the potentiometric sensor was deployed for on-site analysis of the peroxidase activity of <i>Suaeda salsa</i> (L.) Pall. rhizosphere soil under salinity stress, showing excellent sensitivity and anti-interference capability, and retaining promising potential for evaluating the mechanisms and feedback in response to environment stresses.\",\"PeriodicalId\":27,\"journal\":{\"name\":\"Analytical Chemistry\",\"volume\":\"16 1\",\"pages\":\"\"},\"PeriodicalIF\":6.7000,\"publicationDate\":\"2025-03-10\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Analytical Chemistry\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://doi.org/10.1021/acs.analchem.5c00327\",\"RegionNum\":1,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"CHEMISTRY, ANALYTICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Analytical Chemistry","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1021/acs.analchem.5c00327","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
Microdroplet-Mediated Enzyme Activity Enhancement for Potentiometric Sensing of Peroxidase in Soil
On-site measurements of enzyme activity in complex solid matrices such as soil and sediment could offer invaluable insights for the soil health evaluation and sustainable management. As such, sensors for on-site measurements of soil enzyme activity are highly required but remain unachieved to date. Herein, a potentiometric sensor for rapid, direct, and in-field analysis of soil enzyme activity is proposed, in which soil particle separation and soil enzyme extraction can be achieved within a single device. More importantly, the enzyme activity mediated by microdroplets can be greatly enhanced for potentiometric measurements. By using a classic soil redox enzyme-peroxidase and an anti-interference substrate 3,3′,5,5′-tetramethylbenzidine as a model, the potentiometric sensor enables directly measuring peroxidase activity in the field with a detection limit that can reach as low as 10–7 U mL–1 within 5 min. Moreover, the potentiometric sensor was deployed for on-site analysis of the peroxidase activity of Suaeda salsa (L.) Pall. rhizosphere soil under salinity stress, showing excellent sensitivity and anti-interference capability, and retaining promising potential for evaluating the mechanisms and feedback in response to environment stresses.
期刊介绍:
Analytical Chemistry, a peer-reviewed research journal, focuses on disseminating new and original knowledge across all branches of analytical chemistry. Fundamental articles may explore general principles of chemical measurement science and need not directly address existing or potential analytical methodology. They can be entirely theoretical or report experimental results. Contributions may cover various phases of analytical operations, including sampling, bioanalysis, electrochemistry, mass spectrometry, microscale and nanoscale systems, environmental analysis, separations, spectroscopy, chemical reactions and selectivity, instrumentation, imaging, surface analysis, and data processing. Papers discussing known analytical methods should present a significant, original application of the method, a notable improvement, or results on an important analyte.