{"title":"Genome Mining of Epicoccum dendrobii Reveals Diverse Antimicrobial Natural Products","authors":"Anxin Zhang, Xinran Xu, Wen-Bing Yin","doi":"10.1021/acs.jafc.4c11808","DOIUrl":null,"url":null,"abstract":"<i>Epicoccum dendrobii</i>, a biocontrol fungus, produces bioactive metabolites with potential agricultural applications. Targeted genome mining revealed 13 compounds, including three novel polyketides (epiketides A–C, <b>1</b>–<b>3</b>), a new natural product (epiketide D, <b>4</b>), a diketopiperazine (boydine E, <b>5</b>), and eight known analogues <b>6</b>–<b>13</b>. Structures were elucidated via NMR, high-resolution electrospray ionization mass spectrometry (HR-ESI-MS), and computational methods. Compounds <b>1</b>, <b>2</b> and <b>4</b> exhibited moderate antibacterial activity against <i>Bacillus licheniformis</i> (minimum inhibitory concentrations (MICs): 5.5–37.6 μM) and <i>Pseudomonas aeruginosa</i> (MICs: 11.1–20.0 μM). A protoplast transformation system was developed to identify the biosynthetic gene cluster <i>eda</i> responsible for diketopiperazines <b>5</b>–<b>10</b>. Antifungal assays revealed that wild-type <i>E. dendrobii</i> inhibited plant pathogen <i>Botrytis cinerea</i> by 56.03%, while the Δ<i>edaP</i> mutant (lacking diketopiperazines) showed reduced efficacy, implying the role of diketopiperazines in antifungal activity. This study underscores <i>E. dendrobii</i>’s potential as a biocontrol agent and provides insights into its bioactive metabolites for agricultural applications.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"28 1","pages":""},"PeriodicalIF":5.7000,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Agricultural and Food Chemistry","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1021/acs.jafc.4c11808","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRICULTURE, MULTIDISCIPLINARY","Score":null,"Total":0}
Genome Mining of Epicoccum dendrobii Reveals Diverse Antimicrobial Natural Products
Epicoccum dendrobii, a biocontrol fungus, produces bioactive metabolites with potential agricultural applications. Targeted genome mining revealed 13 compounds, including three novel polyketides (epiketides A–C, 1–3), a new natural product (epiketide D, 4), a diketopiperazine (boydine E, 5), and eight known analogues 6–13. Structures were elucidated via NMR, high-resolution electrospray ionization mass spectrometry (HR-ESI-MS), and computational methods. Compounds 1, 2 and 4 exhibited moderate antibacterial activity against Bacillus licheniformis (minimum inhibitory concentrations (MICs): 5.5–37.6 μM) and Pseudomonas aeruginosa (MICs: 11.1–20.0 μM). A protoplast transformation system was developed to identify the biosynthetic gene cluster eda responsible for diketopiperazines 5–10. Antifungal assays revealed that wild-type E. dendrobii inhibited plant pathogen Botrytis cinerea by 56.03%, while the ΔedaP mutant (lacking diketopiperazines) showed reduced efficacy, implying the role of diketopiperazines in antifungal activity. This study underscores E. dendrobii’s potential as a biocontrol agent and provides insights into its bioactive metabolites for agricultural applications.
期刊介绍:
The Journal of Agricultural and Food Chemistry publishes high-quality, cutting edge original research representing complete studies and research advances dealing with the chemistry and biochemistry of agriculture and food. The Journal also encourages papers with chemistry and/or biochemistry as a major component combined with biological/sensory/nutritional/toxicological evaluation related to agriculture and/or food.