蛋白质组学和磷酸化蛋白质组学揭示了饲粮棕榈仁粉对藏羊肌肉嫩度影响的潜在机制

IF 9.8 1区 农林科学 Q1 CHEMISTRY, APPLIED
Ying Ma, Lijuan Han, Shengzhen Hou, Linsheng Gui, Zhenzhen Yuan, Shengnan Sun, Chao Yang, Zhiyou Wang, Baochun Yang
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引用次数: 0

摘要

采用无标签蛋白质组学技术和4D无标签磷酸化蛋白质组学技术,系统分析肌肉压痛蛋白表达调控机制。通过蛋白质组学数据分析筛选出59个差异表达蛋白。磷酸化蛋白质组学分析显示,681个修饰肽水平发生变化,其中235个修饰肽水平对应上调132个蛋白,446个修饰肽水平对应下调253个蛋白。然后,双组学分析进一步预测,肌痛的调节机制主要以糖酵解为基础,通过调节线粒体自噬、细胞凋亡、AMPK和HIF-1信号通路调节肌痛,具体表现为调节Ca2+释放,促进相关蛋白降解肌原纤维蛋白,缩短屠宰后肌糖酵解时间,降低肌糖酵解程度。经WB验证,P53、ENO5、ALDOA、ENDOG和PINK1是压痛调节的潜在因子。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Potential mechanism of dietary palm kernel meal effect on muscle tenderness in Tibetan sheep revealed by proteomics and phosphorylated proteomics
The labe free proteomics technology and 4D labe free phosphorylation proteomics technology was used to systematically analyse the protein expression regulatory mechanisms of muscle tenderness. 59 differentially expressed proteins were screened by proteomic data analysis. Phosphorylated proteomic analysis showed 681 modified peptide levels were changed, of which 235 modified peptide levels corresponded to 132 proteins up-regulated and 446 modified peptide levels corresponded to 253 proteins down-regulated. Then, the two-omics analysis further predicted that the regulatory mechanism of tenderness was mainly based on glycolysis, regulating mitochondrial autophagy, apoptosis, AMPK and HIF-1 signaling pathway to regulate muscle tenderness, which was specifically manifested in the modulation of Ca2+ release to promote the degradation of myofibrillar fibrillar proteins by the relevant proteins, shortening of post-slaughter muscle glycolysis and reducing the degree of muscle glycolysis. Which was verified by WB, P53, ENO5, ALDOA, ENDOG and PINK1 were identified as potential factors for tenderness regulation.
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来源期刊
Food Chemistry
Food Chemistry 工程技术-食品科技
CiteScore
16.30
自引率
10.20%
发文量
3130
审稿时长
122 days
期刊介绍: Food Chemistry publishes original research papers dealing with the advancement of the chemistry and biochemistry of foods or the analytical methods/ approach used. All papers should focus on the novelty of the research carried out.
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