积雪草体外和体内对肠道炎症模型的影响及其活性成分的鉴定。

IF 3.3 2区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY

Journal of the Science of Food and Agriculture Pub Date : 2025-03-06 DOI:10.1002/jsfa.14213

Hyun Young Shin, Yeon Suk Kim, Mi Yeun Joung, Woo Jung Kim, Kwang-Soon Shin, Kwang-Won Yu

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引用次数: 0

摘要

背景：积雪草科（积雪草属）包括约50种，其中许多在传统医学中用于治疗各种炎症性疾病。传统上，积雪草在阿育吠陀医学中被广泛用作草药。亚洲曲霉治疗肠道炎症的疗效尚不清楚。结果：本研究考察了亚洲积血热水提取物（CA-HE）对炎症诱导的肠上皮细胞系和动物模型的影响。CA-HE可有效减轻il -1β诱导的炎症因子和紧密连接（TJ）蛋白。在葡聚糖硫酸钠（DSS）诱导的结肠炎小鼠模型中，口服CA-HE没有引起毒理学作用，并显著缓解结肠炎的主要临床症状。结肠标本检查显示，口服CA-HE可显著预防小鼠dss诱导的炎症和tj相关因子。苏木精、伊红和周期性酸-希夫/阿利新蓝染色结肠切片的组织病理学检查显示，与DSS组相比，CA-HE减轻了隐窝破坏、粘膜下水肿、炎症浸润和粘蛋白分泌。口服CA-HE增加了盲肠和粪便中短链脂肪酸的总水平。最后，UHPLC-MS分析预测CA-HE含有5种酚类化合物（新绿原酸、隐绿原酸、1,5-二咖啡酰奎宁酸（DCQA）、3,5-DCQA和4,5-DCQA）和1种类黄酮（miquelianin）作为关键生物活性成分。结论：CA-HE可能是预防和治疗肠道炎症的备选药物。此外，我们的研究结果为CA-HE作为生物活性成分的使用提供了基础数据。©2025化学工业协会。

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In vitro and in vivo effects of Centella asiatica on intestinal inflammatory models and identification of its bioactive components.

Background: The family Apiaceae (genus Centella) includes approximately 50 species, many of which are used in traditional medicine to treat various inflammatory diseases. Traditionally, Centella asiatica has been widely used as a medicinal herb in Ayurvedic medicine. The efficacy of C. asiatica in treating intestinal inflammation remains poorly explored.

Results: This study investigated the effects of a hot-water extract of C. asiatica (CA-HE) on inflammation-induced intestinal epithelial cell lines and animal models. CA-HE effectively alleviated IL-1β-induced inflammatory factors and tight junction (TJ) proteins. In a dextran sulfate sodium (DSS)-induced mouse model of colitis, oral administration of CA-HE induced no toxicological effects and significantly alleviated major clinical symptoms of colitis. Examination of colon specimens revealed that oral administration of CA-HE significantly prevented DSS-induced inflammatory and TJ-associated factors in mice. Histopathological examination of hematoxylin and eosin- and periodic acid-Schiff/Alcian blue-stained colon sections showed that CA-HE alleviated crypt destruction, submucosal edema, inflammatory infiltration and mucin secretion compared to the DSS group. Oral administration of CA-HE increased the total levels of short-chain fatty acids in the cecum and feces. Finally, UHPLC-MS analysis predicted that CA-HE comprises five phenolic compounds (neochlorogenic acid, cryptochlorogenic acid, 1,5-dicaffeoylquinic acid (DCQA), 3,5-DCQA and 4,5-DCQA) and one flavonoid (miquelianin) as key bioactive components.

Conclusion: Our results suggest that CA-HE could be an alternative candidate to prevent and treat intestinal inflammation. Furthermore, our findings provide foundational data for the use of CA-HE as a bioactive component. © 2025 Society of Chemical Industry.

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来源期刊

Journal of the Science of Food and Agriculture 工程技术-农业综合

CiteScore

8.10

自引率

4.90%

发文量

634

审稿时长

3.1 months

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