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IF 8.5 1区农林科学 Q1 CHEMISTRY, APPLIED

Food Chemistry Pub Date : 2025-03-07 DOI:10.1016/j.foodchem.2025.143777

Yajing Chen, Xiuli Zhang, Zhihong Zheng, Wenhong Cao, Xiaoming Qin, Haisheng Lin, Zhongqin Chen, Huina Zheng, Guoping Zhu, Jialong Gao

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引用次数: 0

摘要

酒精性肝病（ALD）正成为当今世界的一大健康威胁。酒精脱氢酶（ADH）在酒精代谢中发挥着重要作用。太平洋牡蛎（Crassostrea gigas）已被确认为一种食源性肝保护剂。我们首次整合了包括模拟水解、生物信息学预测和分子对接在内的硅学策略，从千头牡蛎中筛选出了ADH激活肽。体外ADH激活活性和表面等离子体共振（SPR）结果表明，该策略可以稳定地筛选出ADH激活肽。我们选择了其中六种肽进一步验证它们对乙醇诱导的 HepG2 细胞的保护作用。其中，肽LQPPR（Leu-Gln-Pro-Pro-Arg）预处理后可提高细胞活力，有效抵抗EtOH诱导的细胞毒性。而且细胞上清液中的转氨酶（ALT、AST）降低，表明细胞损伤得到了改善。结果还显示，LQPPR 预处理细胞的抗氧化能力（SOD、GSH）提高，氧化应激（MDA）降低。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

In silico prospecting of ADH activating peptides from Pacific oyster (Crassostrea gigas) and protective effect on ethanol-induced damage in HepG2 cells

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In silico prospecting of ADH activating peptides from Pacific oyster (Crassostrea gigas) and protective effect on ethanol-induced damage in HepG2 cells

Alcoholic liver disease (ALD) is becoming a major health threat in the world today. Alcohol dehydrogenase (ADH) plays an important role in alcohol metabolism. Pacific oyster (Crassostrea gigas) has been identified as a food-borne hepatoprotective agent. For the first time, we integrated in silico strategy, including simulated hydrolysis, bioinformatic prediction and molecular docking to screen ADH activating peptides from C. gigas. In vitro ADH activation activity and surface plasmon resonance (SPR) results showed that this strategy could stably screen ADH activating peptides. We selected six of them to further verify their protective effect on EtOH-induced HepG2 cells. Among them, peptide LQPPR (Leu-Gln-Pro-Pro-Arg) pretreatment increased cell viability, can effectively resist EtOH-induced cytotoxicity. And the transaminase (ALT, AST) in the cell supernatant decreased, indicating the cell damage was improved. The results also showed that the antioxidant capacity (SOD; GSH) of LQPPR pretreated cells increased, and the oxidative stress (MDA) decreased.

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来源期刊

Food Chemistry 工程技术-食品科技

CiteScore

16.30

自引率

10.20%

发文量

3130

审稿时长

122 days

期刊介绍： Food Chemistry publishes original research papers dealing with the advancement of the chemistry and biochemistry of foods or the analytical methods/ approach used. All papers should focus on the novelty of the research carried out.