Zhiqiang Sun, Yuan Li, Shuangye Li, Siyuan Wang, Siyi Li, Yan Ke, Run Gao, Lei Wang, Zhenming Zhou, Zhe Wu, Zhu Yu
{"title":"植物乳杆菌和纤维素酶对甜高粱青贮发酵特性、碳水化合物组成、体外瘤胃发酵和微生物群落的预处理研究","authors":"Zhiqiang Sun, Yuan Li, Shuangye Li, Siyuan Wang, Siyi Li, Yan Ke, Run Gao, Lei Wang, Zhenming Zhou, Zhe Wu, Zhu Yu","doi":"10.1186/s40538-025-00749-3","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><p>Sweet sorghum (<i>Sorghum bicolo</i>r (L.) Moench) is a popular forage crop in arid and semi-arid areas due to its high drought tolerance, rapid growth, and low production costs. In addition, sweet sorghum, has relatively specific ensiling characteristics because of its high moisture content and sufficient amount of water soluble carbohydrates. Therefore, it is crucial to investigate the raw material characteristics and exogenous additive pretreatment for the regulation of silage quality. This study aimed to evaluate the effects of <i>Lactobacillus plantarum</i> (LP) and cellulase (CEL) on the fermentation profile, carbohydrate composition, in vitro rumen fermentation and microbiota communities of sweet sorghum silages with two different raw material characteristics (fresh sweet sorghum material (MC1); sweet sorghum material wilted outdoors for 6 h after chopping (MC2)).</p><h3>Results</h3><p>In this study, the sweet sorghum treatments were: control (without additives), LP, CEL, or a combination of LP and CEL (LP_CEL). All treated sweet sorghum samples were ensiled for 30 d. A higher content of lactic acid, propionic acid, D-fructose, glucose, sucrose and a lower content of structural carbohydrates were observed in MC1 silage than in MC2 silage. In MC2 silage, the addition of CEL or LP_CEL decreased the content of structural carbohydrates, while it increased the content of D-fructose, glucose, D-arabinose, xylitol, maltose and trehalose (<i>P</i> < 0.05). The in vitro gas production at 48 h was greater in MC1 silage than in MC2 silage, and the addition of CEL or LP_CEL increased the in vitro dry matter digestibility in MC2 silage (<i>P</i> < 0.05). After 30 d of ensiling, disaccharides such as sucrose, maltose and alginate were almost entirely utilized by the microorganisms, while more-consumed monosaccharides included D-fructose, glucose and L-rhamnose. <i>Lactobacillus</i> was the dominant genus (> 80% relative abundance) in all silage samples.</p><h3>Conclusions</h3><p>Raw material characteristics determine carbohydrate composition, in vitro digestibility, and microbial community of sweet sorghum silage. For wilted sweet sorghum with relatively low moisture content, pretreatment with CEL or LP_CEL reduced the structural carbohydrate content, increased the nonstructural carbohydrate content, and improved the digestibility of the silage. However, additives had no obvious impact on enhancing the fermentation quality of sweet sorghum silage for two raw material characteristics.</p><h3>Graphical abstract</h3>\n<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":512,"journal":{"name":"Chemical and Biological Technologies in Agriculture","volume":"12 1","pages":""},"PeriodicalIF":5.2000,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://chembioagro.springeropen.com/counter/pdf/10.1186/s40538-025-00749-3","citationCount":"0","resultStr":"{\"title\":\"Pretreatment of sweet sorghum silages with Lactobacillus plantarum and cellulase with two different raw material characteristics: fermentation profile, carbohydrate composition, in vitro rumen fermentation and microbiota communities\",\"authors\":\"Zhiqiang Sun, Yuan Li, Shuangye Li, Siyuan Wang, Siyi Li, Yan Ke, Run Gao, Lei Wang, Zhenming Zhou, Zhe Wu, Zhu Yu\",\"doi\":\"10.1186/s40538-025-00749-3\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Background</h3><p>Sweet sorghum (<i>Sorghum bicolo</i>r (L.) Moench) is a popular forage crop in arid and semi-arid areas due to its high drought tolerance, rapid growth, and low production costs. In addition, sweet sorghum, has relatively specific ensiling characteristics because of its high moisture content and sufficient amount of water soluble carbohydrates. Therefore, it is crucial to investigate the raw material characteristics and exogenous additive pretreatment for the regulation of silage quality. This study aimed to evaluate the effects of <i>Lactobacillus plantarum</i> (LP) and cellulase (CEL) on the fermentation profile, carbohydrate composition, in vitro rumen fermentation and microbiota communities of sweet sorghum silages with two different raw material characteristics (fresh sweet sorghum material (MC1); sweet sorghum material wilted outdoors for 6 h after chopping (MC2)).</p><h3>Results</h3><p>In this study, the sweet sorghum treatments were: control (without additives), LP, CEL, or a combination of LP and CEL (LP_CEL). All treated sweet sorghum samples were ensiled for 30 d. A higher content of lactic acid, propionic acid, D-fructose, glucose, sucrose and a lower content of structural carbohydrates were observed in MC1 silage than in MC2 silage. In MC2 silage, the addition of CEL or LP_CEL decreased the content of structural carbohydrates, while it increased the content of D-fructose, glucose, D-arabinose, xylitol, maltose and trehalose (<i>P</i> < 0.05). The in vitro gas production at 48 h was greater in MC1 silage than in MC2 silage, and the addition of CEL or LP_CEL increased the in vitro dry matter digestibility in MC2 silage (<i>P</i> < 0.05). After 30 d of ensiling, disaccharides such as sucrose, maltose and alginate were almost entirely utilized by the microorganisms, while more-consumed monosaccharides included D-fructose, glucose and L-rhamnose. <i>Lactobacillus</i> was the dominant genus (> 80% relative abundance) in all silage samples.</p><h3>Conclusions</h3><p>Raw material characteristics determine carbohydrate composition, in vitro digestibility, and microbial community of sweet sorghum silage. For wilted sweet sorghum with relatively low moisture content, pretreatment with CEL or LP_CEL reduced the structural carbohydrate content, increased the nonstructural carbohydrate content, and improved the digestibility of the silage. However, additives had no obvious impact on enhancing the fermentation quality of sweet sorghum silage for two raw material characteristics.</p><h3>Graphical abstract</h3>\\n<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>\",\"PeriodicalId\":512,\"journal\":{\"name\":\"Chemical and Biological Technologies in Agriculture\",\"volume\":\"12 1\",\"pages\":\"\"},\"PeriodicalIF\":5.2000,\"publicationDate\":\"2025-03-05\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://chembioagro.springeropen.com/counter/pdf/10.1186/s40538-025-00749-3\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Chemical and Biological Technologies in Agriculture\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://link.springer.com/article/10.1186/s40538-025-00749-3\",\"RegionNum\":2,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"AGRICULTURE, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Chemical and Biological Technologies in Agriculture","FirstCategoryId":"97","ListUrlMain":"https://link.springer.com/article/10.1186/s40538-025-00749-3","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRICULTURE, MULTIDISCIPLINARY","Score":null,"Total":0}
Pretreatment of sweet sorghum silages with Lactobacillus plantarum and cellulase with two different raw material characteristics: fermentation profile, carbohydrate composition, in vitro rumen fermentation and microbiota communities
Background
Sweet sorghum (Sorghum bicolor (L.) Moench) is a popular forage crop in arid and semi-arid areas due to its high drought tolerance, rapid growth, and low production costs. In addition, sweet sorghum, has relatively specific ensiling characteristics because of its high moisture content and sufficient amount of water soluble carbohydrates. Therefore, it is crucial to investigate the raw material characteristics and exogenous additive pretreatment for the regulation of silage quality. This study aimed to evaluate the effects of Lactobacillus plantarum (LP) and cellulase (CEL) on the fermentation profile, carbohydrate composition, in vitro rumen fermentation and microbiota communities of sweet sorghum silages with two different raw material characteristics (fresh sweet sorghum material (MC1); sweet sorghum material wilted outdoors for 6 h after chopping (MC2)).
Results
In this study, the sweet sorghum treatments were: control (without additives), LP, CEL, or a combination of LP and CEL (LP_CEL). All treated sweet sorghum samples were ensiled for 30 d. A higher content of lactic acid, propionic acid, D-fructose, glucose, sucrose and a lower content of structural carbohydrates were observed in MC1 silage than in MC2 silage. In MC2 silage, the addition of CEL or LP_CEL decreased the content of structural carbohydrates, while it increased the content of D-fructose, glucose, D-arabinose, xylitol, maltose and trehalose (P < 0.05). The in vitro gas production at 48 h was greater in MC1 silage than in MC2 silage, and the addition of CEL or LP_CEL increased the in vitro dry matter digestibility in MC2 silage (P < 0.05). After 30 d of ensiling, disaccharides such as sucrose, maltose and alginate were almost entirely utilized by the microorganisms, while more-consumed monosaccharides included D-fructose, glucose and L-rhamnose. Lactobacillus was the dominant genus (> 80% relative abundance) in all silage samples.
Conclusions
Raw material characteristics determine carbohydrate composition, in vitro digestibility, and microbial community of sweet sorghum silage. For wilted sweet sorghum with relatively low moisture content, pretreatment with CEL or LP_CEL reduced the structural carbohydrate content, increased the nonstructural carbohydrate content, and improved the digestibility of the silage. However, additives had no obvious impact on enhancing the fermentation quality of sweet sorghum silage for two raw material characteristics.
期刊介绍：
Chemical and Biological Technologies in Agriculture is an international, interdisciplinary, peer-reviewed forum for the advancement and application to all fields of agriculture of modern chemical, biochemical and molecular technologies. The scope of this journal includes chemical and biochemical processes aimed to increase sustainable agricultural and food production, the evaluation of quality and origin of raw primary products and their transformation into foods and chemicals, as well as environmental monitoring and remediation. Of special interest are the effects of chemical and biochemical technologies, also at the nano and supramolecular scale, on the relationships between soil, plants, microorganisms and their environment, with the help of modern bioinformatics. Another special focus is the use of modern bioorganic and biological chemistry to develop new technologies for plant nutrition and bio-stimulation, advancement of biorefineries from biomasses, safe and traceable food products, carbon storage in soil and plants and restoration of contaminated soils to agriculture.
This journal presents the first opportunity to bring together researchers from a wide number of disciplines within the agricultural chemical and biological sciences, from both industry and academia. The principle aim of Chemical and Biological Technologies in Agriculture is to allow the exchange of the most advanced chemical and biochemical knowledge to develop technologies which address one of the most pressing challenges of our times - sustaining a growing world population.
Chemical and Biological Technologies in Agriculture publishes original research articles, short letters and invited reviews. Articles from scientists in industry, academia as well as private research institutes, non-governmental and environmental organizations are encouraged.
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