IF 4.4 2区 生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Ji-Yeon Baek, Ji Hun Kim, Na-Kyoung Lee, Hyun-Dong Paik
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引用次数: 0

摘要

慢性阻塞性肺病(COPD)的特点是慢性肺部炎症和细胞凋亡。虽然副微生物对呼吸系统健康的有益作用众所周知,但有关其对慢性阻塞性肺病影响的研究却很有限。在本研究中,我们研究了热杀灭的植物乳杆菌 WB3813 和植物乳杆菌 WB3814 对脂多糖(LPS)诱导的 A549 细胞炎症和细胞凋亡的影响。这两种菌株都表现出了适当的益生特性,并被证实是安全的。结果表明,热杀灭乳酸菌(H-LAB)具有抗炎和抗细胞凋亡作用。虽然暴露于 LPS 会降低细胞的存活率,但用两种 H-LAB 处理对细胞有保护作用。RT-PCR 结果显示,H-LAB 预处理降低了 COPD 相关 mRNA 的表达水平,Western 印迹分析表明下调了激活 NF-κB 和内在凋亡信号通路。此外,LPS 诱导的 IL-6 和 eotaxin 水平升高也有所下降。此外,这些菌株还能明显降低因 LPS 而增加的细胞内 ROS 水平,并促进自由基清除活性。虽然尚未阐明其体内效应,但本研究表明,在肺上皮细胞损伤模型中,热杀死或活的植物乳杆菌 WB3813 和植物乳杆菌 WB3814 具有抗炎和抗细胞凋亡的作用。这些发现为开发改善支气管健康的功能性食品以及在慢性阻塞性肺病治疗中的潜在临床应用提供了实验证据。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Heat-killed Lactiplantibacillus plantarum WB3813 and Lactiplantibacillus plantarum WB3814 Alleviate LPS-Induced Inflammatory Damage and Apoptosis in A549 Cells.

Chronic obstructive pulmonary disease (COPD) is characterized by chronic lung inflammation and apoptosis. Although paraprobiotics are known for their beneficial effects on respiratory health, research on their effect on COPD is limited. In this study, we investigated the effects of heat-killed Lactiplantibacillus plantarum WB3813 and Lact. plantarum WB3814 on inflammation and apoptosis in lipopolysaccharide (LPS)-induced A549 cells. Both strains demonstrated appropriate probiotic properties and were confirmed to be safe. The results showed that heat-killed lactic acid bacteria (H-LAB) exhibited anti-inflammatory and anti-apoptotic effects. Although LPS exposure reduced cell viability, treatment with the two H-LAB demonstrated a protective effect on the cells. RT-PCR results showed that H-LAB pretreatment reduced COPD-related mRNA expression levels, and western blotting analysis indicated downregulation of the activation NF-κB and intrinsic apoptotic signaling pathways. Additionally, elevated levels of IL-6 and eotaxin induced by LPS decreased. Furthermore, the strains significantly lowered intracellular ROS levels, which were increased by LPS, and promoted radical-scavenging activity. Although the in vivo effects have not yet been elucidated, this study demonstrated that heat-killed or live Lact. plantarum WB3813 and Lact. plantarum WB3814 exhibit anti-inflammatory and anti-apoptotic effects in a lung epithelial cell injury model. These findings provide experimental evidence for the development of functional foods to improve bronchial health and for potential clinical applications in COPD treatment.

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来源期刊
Probiotics and Antimicrobial Proteins
Probiotics and Antimicrobial Proteins BIOTECHNOLOGY & APPLIED MICROBIOLOGYMICROB-MICROBIOLOGY
CiteScore
11.30
自引率
6.10%
发文量
140
期刊介绍: Probiotics and Antimicrobial Proteins publishes reviews, original articles, letters and short notes and technical/methodological communications aimed at advancing fundamental knowledge and exploration of the applications of probiotics, natural antimicrobial proteins and their derivatives in biomedical, agricultural, veterinary, food, and cosmetic products. The Journal welcomes fundamental research articles and reports on applications of these microorganisms and substances, and encourages structural studies and studies that correlate the structure and functional properties of antimicrobial proteins.
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