Sarfaraz Hussain, Maratab Ali, Abdel‑Halim Ghazy, Abdullah A. Al-Doss, Kotb A. Attia, Tawaf Ali Shah, Fujun Li
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引用次数: 0
摘要
本研究解决了番茄枯萎病（Fusarium oxysporum f. sp. lycopersici， FOL）引起的番茄枯萎病的关键问题，这是一种严重的真菌病原体，负责全球产量损失。传统的控制措施，如抗性作物品种和化学杀菌剂，由于环境问题和病原体抗性的风险而具有局限性。作为一种可持续的替代方案，本研究旨在探索菌株Sh-17的生物防治潜力，重点研究其脂肽（LPs）对FOL的有效抑制作用。结果本研究证实了从番茄田间获得的Sh-17菌株对FOL的抗真菌能力。通过16S rDNA基因序列分析和表型鉴定，Sh-17为枯草芽孢杆菌Sh-17。在离体培养皿的疾病控制试验中，Sh-17和FOL同时接种番茄种子时，在幼苗中显示出良好的植物生长促进和疾病控制能力。随后，从Sh-17提取的脂肽提取物显示出较强的抗真菌特性，且呈剂量依赖性，在3500µg mL−1浓度下对FOL具有完全抑制作用。此外，我们观察到LPs降低了麦角甾醇的含量，从而影响了质膜的稳定性和总体结构。对Sh-17的基因组DNA进行PCR筛选，发现存在抗真菌LPs生物合成相关基因。此外，LC-MS分析在Sh-17的LPs粗提取物中鉴定出不同的LPs，如表面素、风霉素、iturins、杆菌素和杆菌素衍生物。此外，显微镜分析（荧光/透射电镜）显示，植物病原体与lps相互作用后，菌丝和孢子出现形态异常甚至死亡。枯草杆菌Sh-17对FOL表现出很强的抗真菌特性，并通过保护幼苗免受病原体侵袭来支持幼苗健康。Sh-17产生的LPs通过破坏病原菌的细胞结构，呈剂量依赖性地抑制FOL的生长，是一种有效的番茄枯萎病生物防治剂。图形抽象
Identification of antifungal lipopeptides from Bacillus subtilis Sh-17 targeting Fusarium oxysporum f. sp. lycopersici
Background
This study addresses the critical issue of Fusarium wilt in tomatoes, caused by Fusarium oxysporum f. sp. lycopersici (FOL), a severe fungal pathogen responsible for global yield losses. Conventional control measures, such as resistant crop varieties and chemical fungicides, have limitations due to environmental concerns and the risk of pathogen resistance. As a sustainable alternative, this study aims to explore the biocontrol potential of the bacterial strain Sh-17, focusing on its lipopeptides (LPs) to effectively suppress FOL.
Results
This study demonstrated the antifungal capability of the Sh-17 strain, obtained from a tomato field, against FOL. Through 16S rDNA gene sequence analysis and phenotypic evaluation, Sh-17 was identified as Bacillus subtilis Sh-17. During the disease control assay using in vitro petri dishes, Sh-17 showed promising plant growth-promoting and disease-control capabilities in seedlings when tomato seeds were inoculated with both Sh-17 and FOL. Subsequently, the lipopeptide extract derived from Sh-17 showed strong antifungal properties in a dose-dependent manner, with complete inhibition of FOL at a concentration of 3500 µg mL−1. Furthermore, it was observed that LPs decreased the amount of ergosterol, which affects the stability and general structure of the plasma membrane. The genomic DNA of Sh-17 was subjected to PCR screening, which revealed the presence of genes responsible for the biosynthesis of antifungal LPs. Furthermore, LC–MS analysis identified distinct LPs, such as surfactins, fengycin, iturins, bacilysin, and bacillomycin derivatives in the crude LPs extract of Sh-17. Moreover, microscopic analyses (fluorescent/TEM) demonstrated morphological abnormalities and even death of the hyphae and spores of the phytopathogen upon its interaction with LPs.
Conclusions
B. subtilis Sh-17 exhibits strong antifungal properties against FOL and supports seedlings health by protecting them from pathogen infestation. The LPs produced by Sh-17 inhibit FOL growth in a dose-dependent manner by disrupting the pathogen’s cellular structures and proved to be an effective biocontrol agent against Fusarium wilt in tomatoes.
期刊介绍：
Chemical and Biological Technologies in Agriculture is an international, interdisciplinary, peer-reviewed forum for the advancement and application to all fields of agriculture of modern chemical, biochemical and molecular technologies. The scope of this journal includes chemical and biochemical processes aimed to increase sustainable agricultural and food production, the evaluation of quality and origin of raw primary products and their transformation into foods and chemicals, as well as environmental monitoring and remediation. Of special interest are the effects of chemical and biochemical technologies, also at the nano and supramolecular scale, on the relationships between soil, plants, microorganisms and their environment, with the help of modern bioinformatics. Another special focus is the use of modern bioorganic and biological chemistry to develop new technologies for plant nutrition and bio-stimulation, advancement of biorefineries from biomasses, safe and traceable food products, carbon storage in soil and plants and restoration of contaminated soils to agriculture.
This journal presents the first opportunity to bring together researchers from a wide number of disciplines within the agricultural chemical and biological sciences, from both industry and academia. The principle aim of Chemical and Biological Technologies in Agriculture is to allow the exchange of the most advanced chemical and biochemical knowledge to develop technologies which address one of the most pressing challenges of our times - sustaining a growing world population.
Chemical and Biological Technologies in Agriculture publishes original research articles, short letters and invited reviews. Articles from scientists in industry, academia as well as private research institutes, non-governmental and environmental organizations are encouraged.
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