蓝莓叶酚类化合物对α-淀粉酶和α-葡萄糖苷酶的抑制作用：动力学、作用方式和分子相互作用

IF 3.3 2区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY

Journal of the Science of Food and Agriculture Pub Date : 2025-02-26 DOI:10.1002/jsfa.14187

Han Wu, Xiaoli Liu, Shudong Xie, Jianzhong Zhou, Maria G Corradini, Yue Pan, Xiaozhen Cui

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引用次数: 0

摘要

背景：采用多种技术分析了蓝莓叶多酚（BLPs）与消化酶的相互作用，以了解其对酶动力学和作用方式的抑制作用。结果：3- o -咖啡酰奎宁酸（3-CQA）含量最高。槲皮素（QR）对α-淀粉酶（α-AMY）和α-葡萄糖苷酶（α-GLU）的抑制活性最强。BLP提取物对α-GLU （IC50 = 7.36±0.03 μg mL-1）的抑制作用强于α-AMY （IC50 = 12.52±0.65 μg mL-1）。Stern-Volmer图显示酶荧光强度静态猝灭。α-GLU的猝灭常数和结合常数均高于α-AMY，表明前者对BLP的亲和力更强。在分子水平上研究了3-CQA和QR在BLP中的构象变化。形成的配合物的稳定性顺序为：α-GLU-3-CQA > α-AMY-QR > α-GLU-QR > α-AMY-3-CQA。这一趋势支持了QR对α-AMY构象的影响更大，而3-CQA更有效地改变α-GLU构象的观察。结论：这些发现阐明了BLP对葡萄糖调节酶的抑制机制，为糖尿病的治疗提供了新的见解。©2025化学工业协会。

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Inhibitory effects of phenolic compounds from blueberry leaf on α-amylase and α-glucosidase: kinetics, mode of action, and molecular interactions

Background

The interactions between blueberry leaf polyphenols (BLPs) and digestive enzymes were analyzed using multiple techniques to gain insights into their inhibitory effects on enzyme kinetics and modes of action.

Results

3-O-Caffeoylquinic acid (3-CQA) was the most abundant compound identified. Quercetin (QR) exhibited the strongest inhibitory activity against α-amylase (α-AMY) and α-glucosidase (α-GLU). The BLP extracts acted as typical mixed-type inhibitors for both digestive enzymes, showing stronger inhibition of α-GLU (IC50 = 7.36 ± 0.03 μg mL⁻¹) than α-AMY (IC50 = 12.52 ± 0.65 μg mL⁻¹). Stern–Volmer plots showed static quenching of enzyme fluorescence intensity. The quenching and binding constants of α-GLU were higher than those of α-AMY, showing greater affinity of the former for BLP. The conformational changes of 3-CQA and QR in the BLP were studied at the molecular level. The stability of the complexes formed followed this order: α-GLU-3-CQA > α-AMY-QR > α-GLU-QR > α-AMY-3-CQA. This trend supported the observation that QR had a greater impact on α-AMY conformation, whereas 3-CQA more effectively altered α-GLU.

Conclusion

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来源期刊

Journal of the Science of Food and Agriculture 工程技术-农业综合

CiteScore

8.10

自引率

4.90%

发文量

634

审稿时长

3.1 months

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