α -生育酚在减轻低压缺氧所致黑胃果蝇眼缺陷中的作用机制

IF 2.7 4区 医学 Q2 DEVELOPMENTAL BIOLOGY
Seekha Naik, Smruti Sudha Biswal, Monalisa Mishra
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引用次数: 0

摘要

缺氧或低氧水平与几种病理性疾病有关,包括视网膜病变。视网膜是一个代谢活跃的组织,低氧水平导致视网膜退化。低气压缺氧(HBH)诱导的眼部发育的发育观点仍然是难以捉摸的。果蝇被用作我们的模式生物来研究HBH对眼睛发育和α -生育酚作为潜在抑制剂的影响。为了诱导低氧状态,我们将果蝇暴露在低压(120毫巴)中。组织学染色结果显示,缺氧可引起不同发育阶段果蝇的眼缺损。生化分析揭示了缺氧过程中活性氧(ROS)的存在,导致细胞损伤和DNA损伤。定量PCR结果显示Puf、Wge、Twr基因上调,Rh1、Rh6基因下调参与眼发育。经α -生育酚治疗后,这些缺陷均恢复到正常水平。本研究为理解缺氧引起的眼部发育问题和α -生育酚作为治疗HBH的关键治疗方法提供了基础。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The Mechanism Behind the Therapeutic Role of Alpha-Tocopherol in Mitigating Hypobaric Hypoxia–Induced Eye Defect in Drosophila melanogaster

Hypoxia, or low oxygen levels, is linked to several pathological disorders, including retinopathies. Retina being a metabolically active tissue, low oxygen levels resulted in retinal degradation. The developmental perspective of hypobaric hypoxia (HBH)-induced eye development remains elusive. Drosophila is used as our model organism to investigate the impact of HBH on eye development and alpha-tocopherol as a potential inhibitor. To induce the hypoxic condition, we exposed the Drosophila to hypobaric pressure (120 mbar). Hypoxia induces eye defects in different developmental stages of Drosophila as revealed by histological staining. Biochemical estimation disclosed the presence of reactive oxygen species (ROS) during hypoxia, which led to cellular injury and DNA damage. Quantitative PCR reveals the upregulation of Puf, Wge, and Twr genes and the downregulation of Rh1 and Rh6 involved in eye development. All these defects are brought back to normal levels after treatment with alpha-tocopherol. This research provides a foundation for understanding ocular developmental problems caused by oxygen deprivation and alpha-tocopherol as a crucial therapeutic approach to the treatment of HBH.

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来源期刊
Developmental Neurobiology
Developmental Neurobiology 生物-发育生物学
CiteScore
6.50
自引率
0.00%
发文量
45
审稿时长
4-8 weeks
期刊介绍: Developmental Neurobiology (previously the Journal of Neurobiology ) publishes original research articles on development, regeneration, repair and plasticity of the nervous system and on the ontogeny of behavior. High quality contributions in these areas are solicited, with an emphasis on experimental as opposed to purely descriptive work. The Journal also will consider manuscripts reporting novel approaches and techniques for the study of the development of the nervous system as well as occasional special issues on topics of significant current interest. We welcome suggestions on possible topics from our readers.
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