基于磁性共价有机框架和LC-MS/MS的人血浆中血管紧张素I、II、III和醛固酮的同时富集和超灵敏检测

IF 6.7 1区化学 Q1 CHEMISTRY, ANALYTICAL

Analytical Chemistry Pub Date : 2025-02-19 DOI:10.1021/acs.analchem.4c06426

Shuo Wang, Bin Hu, Han Wang, Xingyi Qi, Zhuo Mi, Ping Su, Huaiyu Zhang, Jianfeng Zhou, Jianchun Chen, Baorong Chen, Jiayi Song, Yi Yang

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引用次数: 0

摘要

血管紧张素I， II和III （Ang I， II, and III）和醛固酮（Aldo）在原发性醛固酮增多症（PA）筛查中发挥重要作用，根据血压调节机制的研究。然而，Ang I、Ang II、Ang III和Aldo在人血浆中浓度较低，且极性不同，这使得目前的检测方法很难同时检测复杂血液样本中的四种分析物。本研究合成了一种新的磁性共价有机骨架（COF），用于富集人血浆中的Ang I、II、III和Aldo，并建立了一种基于磁性共价有机骨架和液相色谱-串联质谱（LC-MS/MS）同时检测四种分析物的新方法。实验结果表明，材料的吸附动力学符合伪二阶模型，富集机制为π-π堆积、静电和氢键相互作用。在优化条件下，所建立的方法具有良好的线性范围（Ang I: 100 ~ 25,000 pg/mL, Ang II: 2 ~ 500 pg/mL, Ang III: 3 ~ 750 pg/mL, Aldo: 20 ~ 5000 pg/mL），低检出限（0.8 ~ 5 pg/mL），高回收率（93.0 ~ 111.3%），且可多次回收，优于已有的研究结果。同时，对20个临床样本的检测结果表明，Ang I、Ang II、Ang III和Aldo均有效，可作为PA筛选的新生物标志物，证明了在真实血液样本中富集这4个靶点的可行性。本实验制备的磁性COF为同时富集Ang I、Ang II、Ang III和Aldo提供了材料设计参考，基于磁性COF和LC-MS/MS开发的新方法为PA筛选提供了新的检测思路，极大地促进了PA疾病诊断的发展，有望在进一步的临床研究中得到应用。

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Simultaneous Enrichment and Ultrasensitive Detection of Angiotensin I, II, and III and Aldosterone in Human Plasma Based on Magnetic Covalent Organic Frameworks and LC-MS/MS

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Simultaneous Enrichment and Ultrasensitive Detection of Angiotensin I, II, and III and Aldosterone in Human Plasma Based on Magnetic Covalent Organic Frameworks and LC-MS/MS

Angiotensin I, II, and III (Ang I, II, and III) and aldosterone (Aldo) play an important role in primary aldosteronism (PA) screening according to the study of the blood pressure regulation mechanism. However, Ang I, Ang II, Ang III, and Aldo are present in human plasma at low concentrations and have different polarities, which make it rather challenging for current detection methods to simultaneously detect four analytes in complex blood samples. In this study, a new magnetic covalent organic framework (COF) was synthesized for the enrichment of Ang I, II, and III and Aldo in human plasma, and a new method for the simultaneous detection of four analytes was developed based on the magnetic COF and liquid chromatography-tandem mass spectrometry (LC-MS/MS). The experimental results demonstrated that the adsorption kinetics of the material conformed to a pseudo-second-order model, and the enrichment mechanisms were π-π stacking, electrostatic, and hydrogen bonding interactions. Under the optimized conditions, the established method has satisfactory linear ranges (Ang I: 100–25,000 pg/mL, Ang II: 2–500 pg/mL, Ang III: 3–750 pg/mL, and Aldo: 20–5000 pg/mL), a low limit of detection (0.8–5 pg/mL), high recoveries (93.0–111.3%), and multiple recycling, which were superior to those reported studies. Meanwhile, the results of testing 20 clinical samples indicated that Ang I, Ang II, Ang III, and Aldo were effective and could be used as new biomarkers for PA screening, which proved the feasibility of enriching the four targets in real blood samples. The prepared magnetic COF in this experiment provided a reference for the material design to simultaneous enrichment of Ang I, Ang II, Ang III, and Aldo, and the developed new method based on the magnetic COF and LC-MS/MS provided a new detection idea for PA screening, which greatly promoted the development of PA disease diagnosis and was expected to be used in further clinical research.

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来源期刊

Analytical Chemistry 化学-分析化学

CiteScore

12.10

自引率

12.20%

发文量

1949

审稿时长

1.4 months

期刊介绍： Analytical Chemistry, a peer-reviewed research journal, focuses on disseminating new and original knowledge across all branches of analytical chemistry. Fundamental articles may explore general principles of chemical measurement science and need not directly address existing or potential analytical methodology. They can be entirely theoretical or report experimental results. Contributions may cover various phases of analytical operations, including sampling, bioanalysis, electrochemistry, mass spectrometry, microscale and nanoscale systems, environmental analysis, separations, spectroscopy, chemical reactions and selectivity, instrumentation, imaging, surface analysis, and data processing. Papers discussing known analytical methods should present a significant, original application of the method, a notable improvement, or results on an important analyte.