间充质干细胞片与聚己内酯纳米纤维垫和明胶泡沫复合可提高大鼠颅骨缺损的成骨能力。

IF 2.2 4区工程技术 Q3 PHARMACOLOGY & PHARMACY

Bioimpacts Pub Date : 2024-04-28 eCollection Date: 2025-01-01 DOI:10.34172/bi.30006

Behnaz Banimohamad-Shotorbani, Reza Rahbarghazi, Seyedhosein Jarolmasjed, Ahmad Mehdipour, Hajar Shafaei

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引用次数: 0

摘要

迄今为止，不同的策略已被用于载细胞生物材料的共移植用于骨组织再生。本研究旨在探讨脂肪源性间充质干细胞（AD-MSC）复合纳米纤维聚己内酯（PCL）垫和明胶泡沫对颅骨骨缺损大鼠成骨的影响。方法：在雄性大鼠颅骨上诱导临界尺寸缺损。动物分为对照组、明胶泡沫组、明胶泡沫/纳米纤维组、明胶泡沫/AD-MSC薄片组和明胶泡沫/PCL纳米纤维/AD-MSC薄片组。3个月后处死大鼠，观察再生率。结果：几乎所有组均表现出骨再生的特性，但Gelfoam/AD-MSC和Gelfoam/PCL纳米纤维/AD-MSC片材组新生骨体积更高(P)。结论：Gelfoam/PCL纳米纤维/AD-MSC片材为颅骨骨缺损的有效成骨提供了合适的平台。

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Combination of mesenchymal stem cell sheet with poly-caprolactone nanofibrous mat and Gelfoam increased osteogenesis capacity in rat calvarial defect.

Introduction: To date, different strategies have been used for co-transplantation of cell-loaded biomaterials for bone tissue regeneration. This study aimed to investigate the osteogenic properties of adipose-derived-mesenchymal stem cell (AD-MSC) sheets combined with nanofibrous poly-caprolactone (PCL) mat and Gelfoam in rats with calvarial bone defect.

Methods: Calvarial critical-size defects were induced in male rats. Animals were classified into Control, Gelfoam, Gelfoam/PCL nanofiber, Gelfoam/AD-MSC sheet, and Gelfoam/PCL nanofiber/AD-MSC sheet groups. After 3 months, rats were sacrificed and the regeneration rate was evaluated.

Results: Almost all groups showed bone regeneration properties, but the volume of newly formed bone was higher in groups that received Gelfoam/AD-MSC and Gelfoam/PCL nanofiber/AD-MSC sheets (P < 0.05). The application of Gelfoam/PCL nanofiber/AD-MSC sheets not only increased bone thickness, bone volume/total bone volume (BV/TV) ratio, strong Hounsfield Unit (HU), but also led to the formation of ossified connective tissue with wrinkled patterns.

Conclusion: The current study indicated that the Gelfoam/PCL nanofiber/AD-MSC sheet provides a suitable platform for effective osteogenesis in calvarial bone defects.

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来源期刊

Bioimpacts Pharmacology, Toxicology and Pharmaceutics-Pharmaceutical Science

CiteScore

4.80

自引率

7.70%

发文量

审稿时长

5 weeks

期刊介绍： BioImpacts (BI) is a peer-reviewed multidisciplinary international journal, covering original research articles, reviews, commentaries, hypotheses, methodologies, and visions/reflections dealing with all aspects of biological and biomedical researches at molecular, cellular, functional and translational dimensions.