IF 2.5 4区 生物学 Q3 CELL BIOLOGY
Jan Rogaliński, Stanisław Przewoźny, Mateusz de Mezer, Anna Markowska, Janina Markowska, Jakub Żurawski
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引用次数: 0

摘要

研究目的我们旨在将 MLH1 和 MSH2 蛋白表达及错配修复(MMR)状态与临床数据(诊断、分级和分期)进行比较。此外,我们还想评估两种免疫组化评估之间的相关性:传统的显微镜检查和通过校准程序进行的计算机分析:我们对 95 例子宫内膜癌病例进行了研究。我们对每个病例都进行了 H+E 染色和两种 MMR 状态蛋白的免疫组化(IHC):MLH1和MSH2。两名独立研究人员对 IHC 进行评估,评分标准为 0 到 ++++。我们根据显微镜下至少一种蛋白无表达的评估结果将病例划分为MMR缺乏病例。为了进行计算机分析,我们使用奥林巴斯的cellSens软件测量了五个视野中以µm2为单位的IHC阳性反应面积:尽管使用了两种不同的评估方法,但我们并未发现诊断、分级、分期、麻风腮蛋白表达和麻风腮状态之间存在任何统计学意义上的显著关系。然而,我们发现计算机分析与半定量显微镜评估之间存在很强的相关性(MLH1 的相关性为 0.59,MSH2 的相关性为 0.76;ppConclusion):我们没有发现 MMR 状态与分级、分期或诊断之间的关系。不过,我们提出了一种使用计算机分析进行免疫组化评估的新方法。它使我们能够利用 IHC 方法开展更客观、更准确的研究。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
MLH1 and MSH2 expression in endometrial cancer - microscopic and computer assessment of immunohistochemical method.

Objective: We aimed to compare MLH1 and MSH2 protein expression and Mismatch repair (MMR) status with clinical data: diagnosis, grading, and staging. Moreover, we wanted to assess any correlation between two immunohistochemical assessments: classic microscopic and computer analysis performed by a calibrated program.

Materials and methods: Our studies were conducted on 95 cases of endometrial cancer. For each, we performed H+E staining and immunohistochemistry (IHC) of two MMR status proteins: MLH1 and MSH2. Two independent researchers assessed IHC on a 0 to ++++ scale. We classified cases as MMR-deficient based on the microscopic assessment of the absence of expression of at least one protein. For computer analysis, we used Olympus cellSens software to measure the positive IHC reaction area in µm2 in five fields of vision.

Results: Despite using two different assessment methods, we did not identify any statistically significant relationship between diagnosis, grading, staging, MMR protein expression, and MMR status. However, we found a strong correlation between computer analysis and semi-quantitative microscopic assessment (r=0.59 for MLH1 and r=0.76 for MSH2; p<0.001 for both). Furthermore, we revealed that computer measurement of the expression area could be a good objective prediction test for microscopic analysis (p<0.001).

Conclusion: We did not find a relationship between MMR status and grading, staging, or diagnosis. However, we present a novel approach to immunohistochemical assessment using computer analysis. It allows us to carry out more objective and accurate studies with the IHC method.

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来源期刊
Histology and histopathology
Histology and histopathology 生物-病理学
CiteScore
3.90
自引率
0.00%
发文量
232
审稿时长
2 months
期刊介绍: HISTOLOGY AND HISTOPATHOLOGY is a peer-reviewed international journal, the purpose of which is to publish original and review articles in all fields of the microscopical morphology, cell biology and tissue engineering; high quality is the overall consideration. Its format is the standard international size of 21 x 27.7 cm. One volume is published every year (more than 1,300 pages, approximately 90 original works and 40 reviews). Each volume consists of 12 numbers published monthly online. The printed version of the journal includes 4 books every year; each of them compiles 3 numbers previously published online.
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