比色法，定量，便携式液晶弹性体生物传感胆固醇和马拉硫磷

IF 6.7 1区化学 Q1 CHEMISTRY, ANALYTICAL

Analytical Chemistry Pub Date : 2025-02-12 DOI:10.1021/acs.analchem.4c05166

Chongyang Mu, Dawei Feng, Mashooq Khan, Haoyang Song, Sundas Munir, Qiongzheng Hu, Li Yu

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引用次数: 0

摘要

胆甾型液晶弹性体（CLCEs）具有一种独特的特性，可以通过调整层与层之间的间距来操纵反射的颜色。这一属性可以为各种物质的现场检测创造一个可靠且具有成本效益的比色传感平台。在这项研究中，CLCE薄膜分别与交联聚丙烯酸（CLCE- paa）或聚二甲氨基乙基甲基丙烯酸酯（CLCE- pda）薄膜偶联来监测胆固醇和马拉硫磷水平。在这两种情况下，颜色变化都是由手机相机记录的，反射波长是分光光度法测量的。对于现场检测，使用智能手机应用程序捕获胶片图像，将颜色数据处理为色调(H)值，并通过分析程序计算相应的被测分析物浓度。胆固醇和马拉硫磷分别在0.2-1.0 mM和1-10,000 ng/mL的线性范围内检测。实际样品分析中胆固醇和马拉硫磷的回收率分别为86-115%和87-122%。该系统为生物和环境样品中胆固醇和马拉硫磷的现场检测提供了实用的解决方案。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Colorimetric, Quantitative, and Portable Liquid Crystal Elastomer Biosensing of Cholesterol and Malathion

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Colorimetric, Quantitative, and Portable Liquid Crystal Elastomer Biosensing of Cholesterol and Malathion

Cholesteric liquid crystal elastomers (CLCEs) possess a unique characteristic that allows the manipulation of the reflected color by adjusting the spacing between layers. This attribute can create a reliable and cost-effective colorimetric sensing platform for the on-site detection of various substances. In this study, CLCE films were coupled with cross-linked poly(acrylic acid) (CLCE-PAA) or poly(dimethylaminoethyl methacrylate) (CLCE-PDA) films to monitor cholesterol and malathion levels, respectively. In both cases, the color change is recorded by a mobile phone camera, and the reflectance wavelength is measured spectrophotometrically. For on-site detection, a smartphone application was used to capture the film’s image, process the color data into hue (H) values, and calculate the corresponding concentration of the tested analyte via an analysis program. Cholesterol and malathion can be detected within a linear range of 0.2–1.0 mM and 1–10,000 ng/mL, respectively. The corresponding recoveries for actual sample analysis were 86–115% and 87–122% for cholesterol and malathion, respectively. This system offers a practical solution for on-site testing of cholesterol and malathion in biological and environmental samples.

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来源期刊

Analytical Chemistry 化学-分析化学

CiteScore

12.10

自引率

12.20%

发文量

1949

审稿时长

1.4 months

期刊介绍： Analytical Chemistry, a peer-reviewed research journal, focuses on disseminating new and original knowledge across all branches of analytical chemistry. Fundamental articles may explore general principles of chemical measurement science and need not directly address existing or potential analytical methodology. They can be entirely theoretical or report experimental results. Contributions may cover various phases of analytical operations, including sampling, bioanalysis, electrochemistry, mass spectrometry, microscale and nanoscale systems, environmental analysis, separations, spectroscopy, chemical reactions and selectivity, instrumentation, imaging, surface analysis, and data processing. Papers discussing known analytical methods should present a significant, original application of the method, a notable improvement, or results on an important analyte.