Developing CRISPR-based electrochemical biosensor method with electrodeposit modification to detect pork DNA in meat products

Pork detection methods in food have been developed to address the growing demand for halal food certification. CRISPR/Cas12 is a pork detection method applicable to raw and processed. The use of Electrochemical biosensor-based in this method allows for quick, affordable, and simple detection. This research was performed to develop a CRISPR-based electrochemical biosensor method to detect pork content in meat products. In this research, analytical parameters were analyzed and the developed method was applied to samples of meat products available in the market. The findings revealed that the detection range for synthetic target DNA was 0.01 nM to 100 nM, with a detection limit of 0.456 nM and a quantification limit of 1.519 nM. The method demonstrated a precision level of 98.30 % and an accuracy of 94.05 %. The method developed in this research showed specificity as it successfully distinguished goat, chicken, duck, and rabbit, as well as between pork and beef contents. The developed method demonstrated the capability to detect pork mixtures at concentrations as low as 0.1 % at 100 nM, with measurements conducted on food products, particularly corned beef, achieving a satisfactory recovery rate of 98.28 % (R value). This method is characterized by its simplicity, operational ease, and reliability, making it well-suited for the analysis of pork in meat-based food samples, thereby supporting the enforcement of halal certification in Muslim-majority countries.