利用聚多巴胺和金属有机骨架协同调节点击化学的高通量比色法免疫检测黄曲霉毒素B1

IF 9.8 1区农林科学 Q1 CHEMISTRY, APPLIED

Food Chemistry Pub Date : 2025-02-10 DOI:10.1016/j.foodchem.2025.143344

Liangqiong Ren , Feng Hong , Junping Wen , Yiping Chen

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引用次数: 0

摘要

在这项研究中，我们利用定制的聚苯乙烯阵列和点击化学诱导的无酶催化，制作了一种高通量、高成本效益的黄曲霉毒素B1检测比色免疫分析法。我们合成了含有聚多巴胺、金属有机框架（UiO-66-NH2）和黄曲霉毒素B1抗原的信号探针。该探针与聚苯乙烯阵列上发生的免疫反应一起，调节溶液中的Cu2+浓度，引发短DNA序列之间的点击化学反应，形成g -四重体DNAzyme，催化显色底物。通过智能手机图像捕捉和分析颜色变化，可以准确量化黄曲霉毒素B1浓度。黄曲霉毒素B1的线性范围为100 pg/mL ~ 50 ng/mL，检出限为26.23 pg/mL。花生样品的平均回收率为81.63 % ~ 112.21 %，玉米样品的平均回收率为80.93 % ~ 113.95 %。该方法与高效液相色谱法对花生样品的测定结果吻合较好。

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High-throughput colorimetry immunoassay for aflatoxin B1 detection using the synergistic regulation of click chemistry by polydopamine and metal-organic framework

In this research, we have crafted a high-throughput, cost-effective colorimetric immunoassay for aflatoxin B₁ detection, utilizing custom-made polystyrene arrays and click chemistry-induced enzyme-free catalysis. We synthesized signal probes incorporating polydopamine, metal-organic framework (UiO-66-NH₂), and aflatoxin B₁ antigen. This probe, in conjunction with the immunoreaction occurring on the polystyrene array, modulates the Cu²⁺ concentration in solution, initiating click chemical reactions among short DNA sequences to form a G-quadruplex DNAzyme that catalyzes a chromogenic substrate. By capturing and analyzing the color variations through smartphone imagery, aflatoxin B₁ concentrations can be accurately quantified. The linear range of aflatoxin B₁ was 100 pg/mL to 50 ng/mL, with the limit of detection of 26.23 pg/mL. The average recoveries were 81.63 % - 112.21 % for peanut samples and 80.93 % - 113.95 % for maize samples. And the method was in good agreement with the HPLC method for peanut samples.

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来源期刊

Food Chemistry 工程技术-食品科技

CiteScore

16.30

自引率

10.20%

发文量

3130

审稿时长

122 days

期刊介绍： Food Chemistry publishes original research papers dealing with the advancement of the chemistry and biochemistry of foods or the analytical methods/ approach used. All papers should focus on the novelty of the research carried out.