四种石榴品种对阿尔茨海默病预防的代谢物谱和神经保护潜力评价。

IF 3.5 2区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY

Journal of the Science of Food and Agriculture Pub Date : 2025-02-07 DOI:10.1002/jsfa.14176

Hana Lee, John M. Chater, Claire C. Heinitz, Yu Wang

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引用次数: 0

摘要

背景：阿尔茨海默病（AD）是一种以认知能力下降和记忆丧失为特征的神经退行性疾病，氧化应激和神经炎症在其进展中起重要作用。目前的治疗方法，如乙酰胆碱酯酶（AChE）抑制剂，提供有限的症状缓解，并可能有副作用。随着人们对副作用更小的功能性食品的兴趣日益浓厚，石榴因其丰富的抗氧化剂含量而受到关注。本研究旨在评价‘奇妙’、‘Phoenicia’、‘Azadi’和‘Eversweet’四个石榴品种的抗氧化和神经保护作用。此外，它还研究了它们的代谢物谱，并预测了可能有助于预防AD的潜在生物活性化合物。结果：“奇妙”品种的总酚含量、总黄酮含量、总花青素含量和自由基清除能力最高。“Phoenicia”和“Wonderful”品种的AChE和BuChE抑制率最高，表明其具有潜在的神经保护作用。PC12细胞实验表明，这些品种显著提高了细胞活力，降低了活性氧和丙二醛水平，并提高了过氧化氢酶活性。代谢组学分析鉴定出141种代谢物，其中“奇妙”品种的代谢物丰度最高。分子对接研究表明，一些代谢物，如紫杉醇素，对AChE和BuChE具有很强的结合亲和力，提示AD治疗的潜力。结论：“奇妙”和“腓尼基”两种石榴品种具有较好的抗氧化和神经保护作用，这可能与它们的高taxifolin含量有关。这些研究结果表明，这些品种可用于开发预防和治疗AD的功能性食品。©2025化学工业协会。

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Evaluation of metabolite profiles and neuroprotective potential in four pomegranate cultivars for Alzheimer's disease prevention

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Evaluation of metabolite profiles and neuroprotective potential in four pomegranate cultivars for Alzheimer's disease prevention

BACKGROUND

Alzheimer's disease (AD) is a neurodegenerative disorder characterized by cognitive decline and memory loss, with oxidative stress and neuroinflammation playing crucial roles in its progression. Current treatments, such as acetylcholinesterase (AChE) inhibitors, offer limited symptomatic relief and can have side effects. With growing interest in functional foods that have fewer side effects, pomegranate (Punica granatum L.) has gained attention because of its rich antioxidant content. The present study aims to evaluate the antioxidant and neuroprotective effects of four pomegranate cultivars: ‘Wonderful’, ‘Phoenicia’, ‘Azadi’ and ‘Eversweet’. Additionally, it investigates their metabolite profiles and predicts potential bioactive compounds that could help prevent AD.

RESULTS

The ‘Wonderful’ cultivar demonstrated the highest levels of total phenolic content, total flavonoid content, total anthocyanin content and radical scavenging activities. The AChE and butyrylcholinesterase (BuChE) inhibition rates were highest in ‘Phoenicia’ and ‘Wonderful’ cultivars, suggesting potential neuroprotective effects. PC12 cell assays indicated that these cultivars significantly improved cell viability, reduced reactive oxygen species and malondialdehyde levels, and also enhanced catalase activity. Metabolomics analysis identified 141 metabolites, with the ‘Wonderful’ cultivar showing the highest metabolite abundance. Molecular docking studies indicated that some metabolites, such as taxifolin, demonstrated strong binding affinities for AChE and BuChE, suggesting potential for AD treatment.

CONCLUSION

The ‘Wonderful’ and ‘Phoenicia’ cultivars demonstrated the most promising antioxidant and neuroprotective effects among the evaluated pomegranates, likely because of their high taxifolin content. These findings suggest that these cultivars could be valuable for developing functional foods aimed at AD prevention and treatment. © 2025 Society of Chemical Industry.

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来源期刊

Journal of the Science of Food and Agriculture 工程技术-农业综合

CiteScore

8.10

自引率

4.90%

发文量

634

审稿时长

3.1 months

期刊介绍： The Journal of the Science of Food and Agriculture publishes peer-reviewed original research, reviews, mini-reviews, perspectives and spotlights in these areas, with particular emphasis on interdisciplinary studies at the agriculture/ food interface. Published for SCI by John Wiley & Sons Ltd. SCI (Society of Chemical Industry) is a unique international forum where science meets business on independent, impartial ground. Anyone can join and current Members include consumers, business people, environmentalists, industrialists, farmers, and researchers. The Society offers a chance to share information between sectors as diverse as food and agriculture, pharmaceuticals, biotechnology, materials, chemicals, environmental science and safety. As well as organising educational events, SCI awards a number of prestigious honours and scholarships each year, publishes peer-reviewed journals, and provides Members with news from their sectors in the respected magazine, Chemistry & Industry . Originally established in London in 1881 and in New York in 1894, SCI is a registered charity with Members in over 70 countries.