黄芪APSN的结构特征及其对免疫失调和组织损伤的潜在治疗作用

IF 6.2 1区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY

Journal of Agricultural and Food Chemistry Pub Date : 2025-02-07 DOI:10.1021/acs.jafc.4c08632

Liqiu Hu, Qili Sun, Zhanpeng Liu, Hanwei Huang, Enze Zhao, Haotian Chen, Yuxun Wu, Yongmei Ge, Dongfang Ouyang, Bin Tang

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引用次数: 0

摘要

针对炎症和免疫介导的疾病对全球健康的影响，本研究着重于从黄芪中纯化和表征一种中性多糖APSN。采用高效凝胶渗透色谱（HPGPC）、傅里叶变换红外光谱（FTIR）和核磁共振（NMR）等手段对APSN的结构特征进行了分析，发现APSN具有高度支链的葡聚糖，其主链和侧链位于O-6位置。另外，我们对APSN的生物活性进行了评估，发现APSN通过抑制RAW264.7巨噬细胞的NF-κB通路显著调节免疫反应，促进HUVECs内皮细胞增殖和血管生成相关基因的表达。这些结果表明APSN作为一种潜在的治疗以免疫失调和组织损伤为特征的疾病的药物，值得进一步研究其机制和临床疗效。

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Structural Characterization of APSN from Astragalus membranaceus and Its Potential Therapeutic Effect on Immune Dysregulation and Tissue Damage

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Structural Characterization of APSN from Astragalus membranaceus and Its Potential Therapeutic Effect on Immune Dysregulation and Tissue Damage

Addressing the global health impact of inflammatory and immune-mediated diseases, this study focused on purifying and characterizing a neutral polysaccharide, APSN, from the Astragalus membranaceus. Employing high-performance gel permeation chromatography (HPGPC), Fourier-transform infrared spectroscopy (FTIR), and nuclear magnetic resonance (NMR) spectroscopy, we elucidated APSN’s structural features, revealing a highly branched glucan with a 1,4-α-d-glucopyranosyl main chain and side chains at the O-6 position. Separately, we assessed APSN’s biological activity, finding that it significantly modulates immune responses by inhibiting the NF-κB pathway in RAW264.7 macrophages and promotes endothelial cell proliferation and angiogenesis-related gene expression in HUVECs. These results position APSN as a potential therapeutic for diseases characterized by immune dysregulation and tissue damage, warranting further investigation of its mechanisms and clinical efficacy.

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来源期刊

Journal of Agricultural and Food Chemistry 农林科学-农业综合

CiteScore

9.90

自引率

8.20%

发文量

1375

审稿时长

2.3 months

期刊介绍： The Journal of Agricultural and Food Chemistry publishes high-quality, cutting edge original research representing complete studies and research advances dealing with the chemistry and biochemistry of agriculture and food. The Journal also encourages papers with chemistry and/or biochemistry as a major component combined with biological/sensory/nutritional/toxicological evaluation related to agriculture and/or food.