用异硫氰酸苯衍生法定性和定量分析植物器官中1-氨基环丙烷-1-羧酸浓度

IF 6.2 1区 农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY
Xinyu Song, Jiayi Liu, Juan Hua* and Shihong Luo*, 
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引用次数: 0

摘要

1-氨基环丙烷-1-羧酸(ACC)是植物激素乙烯的直接前体。采用异硫氰酸苯酯(PITC)衍生化改性方法结合光谱分析分离鉴定了ACC和PITC衍生化反应的三个产物。采用UPLC-MS/MS的MRM+模式建立6-苯基-5-硫氧基-4,6-重氮斯匹罗[2.4]庚烷-7- 1 (PTH-ACC)的分析。选取前体离子(m/z 219.10)处检测到的3个片段离子(m/z 98.10、77.15和72.10)进行定性分析,选取最丰富的产物离子(m/z 98.10)进行定量分析。采用响应面法对主产物PTH-ACC的衍生化反应条件进行了分析和优化,得到最佳衍生化反应条件为反应温度90℃,反应时间1.5 h,甲酸(FA)浓度40%。成功测定了25 ~ 200 mg大米样品中ACC的浓度。此外,还分析了水稻、玉米和棉花幼苗的茎部、种子和根系中ACC的含量。发现ACC浓度具有组织特异性。有趣的是,在番茄和芒果果实的成熟过程中,果实中ACC的浓度呈现先上升后下降的趋势。在黑茄果实中,ACC含量在未成熟阶段最高,随着果实的成熟而降低,从半成熟到成熟阶段保持稳定。这些结果为乙烯的研究提供了可靠的分析技术基础。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Qualitative and Quantitative Analyses of 1-Aminocyclopropane-1-carboxylic Acid Concentrations in Plants Organs Using Phenyl Isothiocyanate Derivatization

Qualitative and Quantitative Analyses of 1-Aminocyclopropane-1-carboxylic Acid Concentrations in Plants Organs Using Phenyl Isothiocyanate Derivatization

1-Aminocyclopropane-1-carboxylic acid (ACC) is a direct precursor of phytohormone ethylene. We used a phenyl isothiocyanate (PITC) derivatization modification method combined with spectrographic analysis to isolate and identify three products of the derivatization reactions of ACC and PITC. The MRM+ mode of UPLC-MS/MS was used to establish the analysis of 6-phenyl-5-thioxo-4,6-diazaspiro[2.4]heptan-7-one (PTH-ACC). Three fragment ions detected at the precursor ion (m/z 219.10) were selected for qualitative analysis (m/z 98.10, 77.15, and 72.10), and the most abundant product ion (m/z 98.10) was selected for quantitative analysis. The conditions for the derivatization reaction for the main product PTH-ACC were analyzed and optimized using response surface methodology, resulting in the optimal derivatization conditions being a reaction temperature of 90 °C, a reaction time of 1.5 h, and a formic acid (FA) concentration of 40%. ACC concentrations in 25–200 mg rice samples was successfully determined. Moreover, the ACC concentrations in the shoots, seeds, and roots of rice, maize, and cotton seedlings were all analyzed. The ACC concentrations were found to exhibit tissue specificity. Interestingly, during the ripening process in tomato and mango fruits, the concentrations of ACC in the fruits showed an initial increase, followed by a decrease. In black nightshade fruits, ACC content was the highest in the immature stage, decreased with fruit ripening, and remained stable from the semimature to mature stages. These results provide a reliable analysis technology foundation for the study of ethylene.

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来源期刊
Journal of Agricultural and Food Chemistry
Journal of Agricultural and Food Chemistry 农林科学-农业综合
CiteScore
9.90
自引率
8.20%
发文量
1375
审稿时长
2.3 months
期刊介绍: The Journal of Agricultural and Food Chemistry publishes high-quality, cutting edge original research representing complete studies and research advances dealing with the chemistry and biochemistry of agriculture and food. The Journal also encourages papers with chemistry and/or biochemistry as a major component combined with biological/sensory/nutritional/toxicological evaluation related to agriculture and/or food.
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