产pufa微藻Schizochytrium sp. ATCC 20888中EPA和DHA比例的调控机制：从生物合成和储存分配角度

IF 6.2 1区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY

Journal of Agricultural and Food Chemistry Pub Date : 2025-02-05 DOI:10.1021/acs.jafc.4c12478

Yaqi Xu, Zhao Zhang, Yanqing Bian, Yuanhao Wang, Ziliang Deng, Rui Luo, Weijia Li, Jingyi Yan, Baohua Zhao, Dongzhe Sun

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引用次数: 0

摘要

Schizochytrium sp. ATCC 20888是工业生产多不饱和脂肪酸（PUFA）的重要物种。本研究从生物合成和储存分布两个方面探讨了影响二十碳五烯酸（EPA）和二十二碳六烯酸（DHA）比例的调控机制。EPA和DHA具有不同的积累模式：EPA的比例随着时间的增加而增加，而DHA在48 h时达到峰值。EPA在对数期主要整合到甘油三酯中，在固定期主要整合到磷脂酰胆碱中。转录组分析表明，EPA的合成涉及脂肪酸合成酶-延长酶/去饱和酶系统，而DHA主要依赖于PUFA合成酶。关键酶ELOVL7、二酰基甘油酰基转移酶（g10562）和溶血磷脂酰转移酶（g8836和g7540）与EPA产量呈正相关，突出了它们在EPA生物合成和储存中的作用。此外，磷酸antetheine adenylyl transferase （PPAT/COASY）和adp -核糖基化因子1_2 （ARF1_2）被确定为PUFA比例的潜在调节因子。该研究为schizochytrium PUFA的遗传优化提供了新的思路。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Regulatory Mechanisms of EPA and DHA Proportions in a PUFA-Producing Microalga, Schizochytrium sp. ATCC 20888: From the Biosynthesis and Storage Distribution Aspects

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Regulatory Mechanisms of EPA and DHA Proportions in a PUFA-Producing Microalga, Schizochytrium sp. ATCC 20888: From the Biosynthesis and Storage Distribution Aspects

Schizochytrium sp. ATCC 20888 is an important species for industrial polyunsaturated fatty acids (PUFA) production. This study investigated the regulatory mechanisms affecting the proportions of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) in terms of biosynthesis and storage distribution. EPA and DHA possessed different accumulation patterns: EPA proportion increased over time, while DHA peaked at 48 h. EPA was predominantly integrated into triacylglycerol during the logarithmic phase and phosphatidylcholine during the stationary phase. Transcriptome analysis revealed that EPA synthesis involved the fatty acid synthase–elongase/desaturase system, while DHA depended mainly on PUFA synthase. Key enzymes, including elongase ELOVL7, diacylglycerol acyltransferase (g10562), and lysophosphatidylcholine acyltransferases (g8836 and g7540), show a positive correlation with EPA yield, highlighting their roles in its biosynthesis and storage. Additionally, phosphopantetheine adenylyl transferase (PPAT/COASY) and ADP-ribosylation factor 1_2 (ARF1_2) were identified as potential regulators of PUFA proportions. This study provided insights for genetic optimization of PUFA production inSchizochytrium.

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来源期刊

Journal of Agricultural and Food Chemistry 农林科学-农业综合

CiteScore

9.90

自引率

8.20%

发文量

1375

审稿时长

2.3 months

期刊介绍： The Journal of Agricultural and Food Chemistry publishes high-quality, cutting edge original research representing complete studies and research advances dealing with the chemistry and biochemistry of agriculture and food. The Journal also encourages papers with chemistry and/or biochemistry as a major component combined with biological/sensory/nutritional/toxicological evaluation related to agriculture and/or food.