采后损伤处理对黄芩毛状根培养物黄酮产量和生物活性的影响

IF 5.2 2区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY

Chemical and Biological Technologies in Agriculture Pub Date : 2025-01-28 DOI:10.1186/s40538-024-00715-5

Hyeon Ji Yeo, Su Young Shin, Sang Un Park, Chang Ha Park

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引用次数: 0

摘要

黄芩根中含有黄芩苷、黄芩苷和黄芩苷等黄酮类化合物，具有很强的生物学效应。因此，这种植物被认为是毛状根系应用的最佳植物材料。结果利用野生根状农杆菌R1000对黄芩叶片进行体外培养，获得了天然转基因生物毛状根，并研究了采后损伤处理对毛状根黄酮产量和体外抗菌性能的影响。用高效液相色谱法（HPLC）测定毛状根中黄芩苷、黄芩苷和黄芩苷的含量。结果表明，黄芩毛状根中黄酮类化合物含量在处理6、12、24、48、72和96 h后呈时间依赖性增加。其中，三种黄酮的产量在暴露于伤害应激96 h后最高。此外，测定了两个时间点（对照和暴露96 h后）根特异性黄酮通路相关基因（SbPAL1、SbPAL2、SbPAL3、SbCCL7、SbCHS2、SbCHI、SbFNS2-2、SbCYP82D1.1和SbF8H）的表达水平。损伤应激后SbPAL1、SbCHS2、SbCHI和sbyp82d1.1的表达水平显著升高。对7种正常病原菌、2种多重耐药病原菌和1种病原菌进行了抗菌观察。黄芩苷、黄芩苷、黄芩苷含量高的黄芩毛状根对这些细菌的抑制区大小大于黄酮含量低的黄芩毛状根。除抗菌活性外，伤后毛状根具有较强的抗炎和抗氧化活性。结论采后损伤处理是提高黄芩毛状根培养物黄酮产量和抗菌活性的良好策略。图形抽象

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Postharvest wounding treatment influences flavone production and biological activities of the hairy root cultures of Scutellaria baicalensis

Background

Scutellaria baicalensis roots contain root-specific flavones (baicalin, baicalein, and wogonin) exhibiting strong biological effects. Hence, this plant is considered the best plant material for hairy root system applications.

Results

In this study, we obtained hairy roots, which are considered natural genetically modified organisms, from S. baicalensis leaves in vitro using wild Agrobacterium rhizogenes (A. rhizogenes) R1000 and investigated the effects of postharvest wounding treatment on their flavone production and in vitro antimicrobial properties. Hairy roots were exposed to wounding stress, and the levels of baicalin, baicalein, and wogonin were determined using high-performance liquid chromatography (HPLC). We found that the levels of these flavones in S. baicalensis hairy roots increased after 6, 12, 24, 48, 72, and 96 h of exposure in a time-dependent manner. In particular, the highest production of three flavones was reported after exposure to 96 h of wounding stress. Furthermore, the expression levels of genes involved in root-specific flavone pathways (SbPAL1, SbPAL2, SbPAL3, SbCCL7, SbCHS2, SbCHI, SbFNS2-2, SbCYP82D1.1, and SbF8H) were determined at two time points (control and after 96 h of exposure). Expression levels of SbPAL1, SbCHS2, SbCHI, and SbCYP82D1.1 were significantly increased following exposure to wounding stress. Antimicrobials were observed with seven normal pathogens, two multidrug-resistant pathogens, and one pathogenic yeast. Moreover, the inhibition zone sizes of these bacteria were larger in the wounded S. baicalensis hairy roots with higher levels of baicalin, baicalein, and wogonin than in those with lower levels of these flavones. In addition to antimicrobial activities, the wounded hairy roots exhibited stronger anti-inflammatory and antioxidant activities than the controls.

Conclusions

Our results indicate that postharvest wounding treatment is a good strategy to increase flavone production and enhance the antibacterial activity of S. baicalensis hairy root cultures.

Graphic Abstract

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来源期刊

Chemical and Biological Technologies in Agriculture Biochemistry, Genetics and Molecular Biology-Biotechnology

CiteScore

6.80

自引率

3.00%

发文量

审稿时长

15 weeks

期刊介绍： Chemical and Biological Technologies in Agriculture is an international, interdisciplinary, peer-reviewed forum for the advancement and application to all fields of agriculture of modern chemical, biochemical and molecular technologies. The scope of this journal includes chemical and biochemical processes aimed to increase sustainable agricultural and food production, the evaluation of quality and origin of raw primary products and their transformation into foods and chemicals, as well as environmental monitoring and remediation. Of special interest are the effects of chemical and biochemical technologies, also at the nano and supramolecular scale, on the relationships between soil, plants, microorganisms and their environment, with the help of modern bioinformatics. Another special focus is the use of modern bioorganic and biological chemistry to develop new technologies for plant nutrition and bio-stimulation, advancement of biorefineries from biomasses, safe and traceable food products, carbon storage in soil and plants and restoration of contaminated soils to agriculture. This journal presents the first opportunity to bring together researchers from a wide number of disciplines within the agricultural chemical and biological sciences, from both industry and academia. The principle aim of Chemical and Biological Technologies in Agriculture is to allow the exchange of the most advanced chemical and biochemical knowledge to develop technologies which address one of the most pressing challenges of our times - sustaining a growing world population. Chemical and Biological Technologies in Agriculture publishes original research articles, short letters and invited reviews. Articles from scientists in industry, academia as well as private research institutes, non-governmental and environmental organizations are encouraged.