化学发光探针通过焦谷氨酸氨基肽酶-1激活增强肾缺血再灌注损伤的可视化

IF 6.7 1区 化学 Q1 CHEMISTRY, ANALYTICAL
Zhongkun Wang, Tingting Geng, Xiaoyi Yue, Zhixiong Zheng, Wei Zhang, Zhen Pan, Qunlin Zhang* and Xiang Shi*, 
{"title":"化学发光探针通过焦谷氨酸氨基肽酶-1激活增强肾缺血再灌注损伤的可视化","authors":"Zhongkun Wang,&nbsp;Tingting Geng,&nbsp;Xiaoyi Yue,&nbsp;Zhixiong Zheng,&nbsp;Wei Zhang,&nbsp;Zhen Pan,&nbsp;Qunlin Zhang* and Xiang Shi*,&nbsp;","doi":"10.1021/acs.analchem.4c0583810.1021/acs.analchem.4c05838","DOIUrl":null,"url":null,"abstract":"<p >The absence of an effective imaging tool for diagnosing renal ischemia-reperfusion injury (RIRI) severely delays its treatment, and currently, no definitive clinical interventions are available. Pyroglutamate aminopeptidase-1 (PGP-1), a potential inflammatory cytokine, has shown considerable potential as a biomarker for tracing the inflammatory process in vivo. However, its exact role in the enhanced visualization of RIRI in complex biological systems has yet to be fully established. Chemiluminescence imaging (CLI) has proven to be one of the most promising diagnostic methods due to its ultrahigh-contrast imaging capabilities compared to fluorescence imaging. In this study, we developed an activatable Schaap’s dioxetane chemiluminescent probe (PGP–PD) to explore the potential of PGP-1 as a marker for CLI of renal injury following ischemia-reperfusion, with the goal of achieving high-contrast in situ diagnostics for RIRI. In vitro, PGP–PD exhibited exceptional selectivity for exogenous PGP-1 and remarkable sensitivity, with a detection limit as low as 2.244 ng/mL. Moreover, in vivo studies successfully demonstrated a positive correlation between the RIRI and PGP-1 level. Notably, in situ imaging with PGP–PD generated a significant chemiluminescent signal within the RIRI-kidney, providing an exceptionally high contrast between injured and normal kidney tissue (∼9.4-fold) in the RIRI mouse model. We anticipate that this work may offer a valuable biomarker (PGP-1) and a powerful imaging tool for improving RIRI in situ diagnosis, thereby aiding treatment planning and surgical outcomes for RIRI patients.</p>","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"97 4","pages":"2367–2374 2367–2374"},"PeriodicalIF":6.7000,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Chemiluminescent Probe for Enhanced Visualization of Renal Ischemia-Reperfusion Injury via Pyroglutamate Aminopeptidase-1 Activation\",\"authors\":\"Zhongkun Wang,&nbsp;Tingting Geng,&nbsp;Xiaoyi Yue,&nbsp;Zhixiong Zheng,&nbsp;Wei Zhang,&nbsp;Zhen Pan,&nbsp;Qunlin Zhang* and Xiang Shi*,&nbsp;\",\"doi\":\"10.1021/acs.analchem.4c0583810.1021/acs.analchem.4c05838\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p >The absence of an effective imaging tool for diagnosing renal ischemia-reperfusion injury (RIRI) severely delays its treatment, and currently, no definitive clinical interventions are available. Pyroglutamate aminopeptidase-1 (PGP-1), a potential inflammatory cytokine, has shown considerable potential as a biomarker for tracing the inflammatory process in vivo. However, its exact role in the enhanced visualization of RIRI in complex biological systems has yet to be fully established. Chemiluminescence imaging (CLI) has proven to be one of the most promising diagnostic methods due to its ultrahigh-contrast imaging capabilities compared to fluorescence imaging. In this study, we developed an activatable Schaap’s dioxetane chemiluminescent probe (PGP–PD) to explore the potential of PGP-1 as a marker for CLI of renal injury following ischemia-reperfusion, with the goal of achieving high-contrast in situ diagnostics for RIRI. In vitro, PGP–PD exhibited exceptional selectivity for exogenous PGP-1 and remarkable sensitivity, with a detection limit as low as 2.244 ng/mL. Moreover, in vivo studies successfully demonstrated a positive correlation between the RIRI and PGP-1 level. Notably, in situ imaging with PGP–PD generated a significant chemiluminescent signal within the RIRI-kidney, providing an exceptionally high contrast between injured and normal kidney tissue (∼9.4-fold) in the RIRI mouse model. We anticipate that this work may offer a valuable biomarker (PGP-1) and a powerful imaging tool for improving RIRI in situ diagnosis, thereby aiding treatment planning and surgical outcomes for RIRI patients.</p>\",\"PeriodicalId\":27,\"journal\":{\"name\":\"Analytical Chemistry\",\"volume\":\"97 4\",\"pages\":\"2367–2374 2367–2374\"},\"PeriodicalIF\":6.7000,\"publicationDate\":\"2025-01-17\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Analytical Chemistry\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://pubs.acs.org/doi/10.1021/acs.analchem.4c05838\",\"RegionNum\":1,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"CHEMISTRY, ANALYTICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Analytical Chemistry","FirstCategoryId":"92","ListUrlMain":"https://pubs.acs.org/doi/10.1021/acs.analchem.4c05838","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
引用次数: 0

摘要

缺乏诊断肾缺血再灌注损伤(RIRI)的有效成像工具严重延误了其治疗,目前尚无明确的临床干预措施。焦谷氨酸氨基肽酶-1 (PGP-1)是一种潜在的炎症细胞因子,已显示出相当大的潜力作为生物标志物来追踪体内炎症过程。然而,它在复杂生物系统中增强RIRI可视化中的确切作用尚未完全确定。与荧光成像相比,化学发光成像(CLI)具有超高对比度的成像能力,已被证明是最有前途的诊断方法之一。在这项研究中,我们开发了一种可激活的Schaap 's二氧乙烷化学发光探针(PGP-PD),以探索PGP-1作为缺血-再灌注后肾损伤CLI标志物的潜力,目的是实现RIRI的高对比度原位诊断。在体外,PGP-PD对外源PGP-1具有极强的选择性和敏感性,检出限低至2.244 ng/mL。此外,体内研究成功地证明了RIRI和PGP-1水平之间的正相关。值得注意的是,PGP-PD原位成像在RIRI肾脏内产生了显著的化学发光信号,在RIRI小鼠模型中提供了损伤和正常肾脏组织之间异常高的对比度(约9.4倍)。我们预计这项工作可能为改善RIRI原位诊断提供有价值的生物标志物(PGP-1)和强大的成像工具,从而帮助RIRI患者的治疗计划和手术结果。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Chemiluminescent Probe for Enhanced Visualization of Renal Ischemia-Reperfusion Injury via Pyroglutamate Aminopeptidase-1 Activation

Chemiluminescent Probe for Enhanced Visualization of Renal Ischemia-Reperfusion Injury via Pyroglutamate Aminopeptidase-1 Activation

The absence of an effective imaging tool for diagnosing renal ischemia-reperfusion injury (RIRI) severely delays its treatment, and currently, no definitive clinical interventions are available. Pyroglutamate aminopeptidase-1 (PGP-1), a potential inflammatory cytokine, has shown considerable potential as a biomarker for tracing the inflammatory process in vivo. However, its exact role in the enhanced visualization of RIRI in complex biological systems has yet to be fully established. Chemiluminescence imaging (CLI) has proven to be one of the most promising diagnostic methods due to its ultrahigh-contrast imaging capabilities compared to fluorescence imaging. In this study, we developed an activatable Schaap’s dioxetane chemiluminescent probe (PGP–PD) to explore the potential of PGP-1 as a marker for CLI of renal injury following ischemia-reperfusion, with the goal of achieving high-contrast in situ diagnostics for RIRI. In vitro, PGP–PD exhibited exceptional selectivity for exogenous PGP-1 and remarkable sensitivity, with a detection limit as low as 2.244 ng/mL. Moreover, in vivo studies successfully demonstrated a positive correlation between the RIRI and PGP-1 level. Notably, in situ imaging with PGP–PD generated a significant chemiluminescent signal within the RIRI-kidney, providing an exceptionally high contrast between injured and normal kidney tissue (∼9.4-fold) in the RIRI mouse model. We anticipate that this work may offer a valuable biomarker (PGP-1) and a powerful imaging tool for improving RIRI in situ diagnosis, thereby aiding treatment planning and surgical outcomes for RIRI patients.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Analytical Chemistry
Analytical Chemistry 化学-分析化学
CiteScore
12.10
自引率
12.20%
发文量
1949
审稿时长
1.4 months
期刊介绍: Analytical Chemistry, a peer-reviewed research journal, focuses on disseminating new and original knowledge across all branches of analytical chemistry. Fundamental articles may explore general principles of chemical measurement science and need not directly address existing or potential analytical methodology. They can be entirely theoretical or report experimental results. Contributions may cover various phases of analytical operations, including sampling, bioanalysis, electrochemistry, mass spectrometry, microscale and nanoscale systems, environmental analysis, separations, spectroscopy, chemical reactions and selectivity, instrumentation, imaging, surface analysis, and data processing. Papers discussing known analytical methods should present a significant, original application of the method, a notable improvement, or results on an important analyte.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信