基于收缩通道液滴回注芯片的SARS-CoV-2和H1N1简单集成ddpcr检测系统

IF 6.7 1区化学 Q1 CHEMISTRY, ANALYTICAL

Analytical Chemistry Pub Date : 2025-01-23 DOI:10.1021/acs.analchem.4c05201

Fengyi Liu, Anle Ge, Xixian Wang, Fei Wu, Jian Xu, Bo Ma

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引用次数: 0

摘要

液滴微流体是数字液滴聚合酶链反应（ddPCR）应用的一种强有力的方法。然而，液滴检测过程中液滴控制精确、外设笨重、操作步骤多等问题阻碍了ddPCR的广泛应用。本文提出了一种收缩通道式液滴回注芯片，实现了液滴的自分离和间隔检测。在此基础上，建立了简单集成式ddPCR （Simple Integrated ddPCR, SI-ddPCR）系统，包括基于表面润湿的液滴生成、管加热和液滴信号检测。为了评估该系统的性能，我们使用双链ddpcr同时对SARS-CoV-2和H1N1进行定量。在101 ~ 104 copies/μL范围内线性良好（R2 = 0.999）。采用SI-ddPCR系统对20份拭子标本的临床标本进行了SARS-CoV-2和H1N1的检测，准确率为97.5%。因此，开发的SI-ddPCR系统提供了简单的液滴检测，消除了复杂的外围设备和多步骤操作，并有望成为一种便携式，低成本，易于部署的高精度ddPCR工具箱。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

A Contracted Channel Droplet Reinjection Chip-Based Simple Integrated ddpcr System for SARS-CoV-2 and H1N1 Detection

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A Contracted Channel Droplet Reinjection Chip-Based Simple Integrated ddpcr System for SARS-CoV-2 and H1N1 Detection

Droplet microfluidics is a powerful method for digital droplet polymerase chain reaction (ddPCR) applications. However, precise droplet control, bulky peripherals, and multistep operation usually required in droplet detection process hinder the broad application of ddPCR. Here, a contracted channel droplet reinjection chip is presented, where droplets can be self-separated and detected one by one at intervals. Based on that, a Simple Integrated ddPCR (SI-ddPCR) system is established, including surface-wetting-based droplet generation, tube heating, and droplet signal detection. To assess the system’s performance, we quantified SARS-CoV-2 and H1N1 simultaneously using duplex-ddPCR. The results exhibited a good linearity (R² = 0.999) at concentrations ranging from 10¹ to 10⁴ copies/μL. By employing the SI-ddPCR system, we detected SARS-CoV-2 and H1N1 in clinical samples isolated from 20 swab specimens with an accuracy of 97.5%. Thus, the developed SI-ddPCR system offers simple droplet detection, eliminates complicated peripherals and multistep operations, and promises to be a portable, low-cost, and easy-to-deploy toolbox for high-accuracy ddPCR.

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来源期刊

Analytical Chemistry 化学-分析化学

CiteScore

12.10

自引率

12.20%

发文量

1949

审稿时长

1.4 months

期刊介绍： Analytical Chemistry, a peer-reviewed research journal, focuses on disseminating new and original knowledge across all branches of analytical chemistry. Fundamental articles may explore general principles of chemical measurement science and need not directly address existing or potential analytical methodology. They can be entirely theoretical or report experimental results. Contributions may cover various phases of analytical operations, including sampling, bioanalysis, electrochemistry, mass spectrometry, microscale and nanoscale systems, environmental analysis, separations, spectroscopy, chemical reactions and selectivity, instrumentation, imaging, surface analysis, and data processing. Papers discussing known analytical methods should present a significant, original application of the method, a notable improvement, or results on an important analyte.