The impact of a high-carbohydrate diet on the cognitive behavior of mice in a low-pressure, low-oxygen environment.

The effectiveness of high-carbohydrate diets (HCD) on cognitive impairment is still being debated. To clarify the impact of HCD on the cognitive behavior of mice under low-pressure hypoxic conditions, we studied 24 mice in different environments while subjecting them to dietary intervention for 5 weeks, and conducting behavioral tests. Under low-pressure hypoxic conditions, HCD intervention reversed the decline in spatial learning and memory abilities in mice caused by hypoxia, ameliorated pathological brain damage, and restored the integrity of the intestinal mucosa. We also identified differences in the microbial community. Under low-pressure hypoxic conditions, the intestinal abundance of Parasutterella in mice decreased, the abundance of harmful bacteria such as Desulfovibrio increased, and apoptosis was more prevalent, possibly explaining the observed decreases in glutathione peroxidase activity and brain-derived neurotrophic factor (BDNF) expression in the brain. HCD intervention increased the intestinal abundance of Bifidobacterium in hypoxic mice, reduced the abundances of Desulfovibrio and Faecalibaculum, and played antioxidant roles by lowering malondialdehyde levels and increasing superoxide dismutase activity in the brain by metabolizing amino acids and lipids. HCD also upregulated hippocampal BDNF levels and downregulated caspase 3. Collectively, these results are important because they help explain how HCD intervention can reduce hypoxia-induced damage to brain function.