苹果果实水核代谢物积累途径：综合转录组和代谢组分析

IF 3.9 2区农林科学 Q1 HORTICULTURE

Scientia Horticulturae Pub Date : 2025-01-09 DOI:10.1016/j.scienta.2025.113954

Yongli Jiang, Maiqi Zhang, Xinyi Yin, Zhijia Liu, Linyan Zhou, Xiaosong Hu, Junjie Yi

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引用次数: 0

摘要

水核苹果的独特形态和风味引起了人们的极大兴趣，但区分水核和非水核组织的分子机制尚不清楚。本研究通过整合代谢组学和转录组学数据来研究苹果水核形成的途径。我们的研究结果表明，水核苹果的糖代谢优先富集，包括高度积累的山梨醇。山梨糖醇脱氢酶的表达上调与水核的形成无关。水核诱导蔗糖合成相关基因（如蔗糖-磷酸磷酸酶）的表达水平上调，而抑制葡萄糖分解相关基因（己糖激酶）的表达。此外，水核苹果的钙含量显著高于水核苹果。此外，植物与病原体的相互作用被下调。水核还与防御和冷应激相关基因的表达水平有关。这些发现为水核的分子基础提供了新的见解，为有针对性的采后干预奠定了基础，以提高苹果的品质和储存弹性。

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Metabolite accumulation pathway of watercore in apple fruit: An integrative transcriptome and metabolome analysis

The distinctive morphology and flavor of watercored apples have drawn significant interest, yet the molecular mechanisms differentiating watercored and non-watercored tissues remain unclear. This study investigates the pathways involved in apple watercore formation by integrating metabolomic and transcriptomic data. Our findings revealed that sugar metabolism was prioritized enriched in watercored apple, including highly accumulated sorbitol. Up-regulated expression of sorbitol dehydrogenase showed no relationship between its activity and watercore formation. Watercore induced up-regulated expression levels of sucrose synthesis-related genes (e.g., sucrose-phosphate phosphatase), whereas it inhibited the expression of glucose decomposition-related genes (hexokinase). Additionally, significantly higher calcium content was detected in watercored apples. Furthermore, plant-pathogen interaction was down-regulated. Watercore was also associated with the expression level of genes related to defense and cold stress. These findings offer new insights into the molecular basis of watercore, laying the groundwork for targeted postharvest interventions to improve apple quality and storage resilience.

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来源期刊

Scientia Horticulturae 农林科学-园艺

CiteScore

8.60

自引率

4.70%

发文量

796

审稿时长

47 days

期刊介绍： Scientia Horticulturae is an international journal publishing research related to horticultural crops. Articles in the journal deal with open or protected production of vegetables, fruits, edible fungi and ornamentals under temperate, subtropical and tropical conditions. Papers in related areas (biochemistry, micropropagation, soil science, plant breeding, plant physiology, phytopathology, etc.) are considered, if they contain information of direct significance to horticulture. Papers on the technical aspects of horticulture (engineering, crop processing, storage, transport etc.) are accepted for publication only if they relate directly to the living product. In the case of plantation crops, those yielding a product that may be used fresh (e.g. tropical vegetables, citrus, bananas, and other fruits) will be considered, while those papers describing the processing of the product (e.g. rubber, tobacco, and quinine) will not. The scope of the journal includes all horticultural crops but does not include speciality crops such as, medicinal crops or forestry crops, such as bamboo. Basic molecular studies without any direct application in horticulture will not be considered for this journal.