Hesperidin enhances broiler growth performance by augmenting gastric acid secretion via the proton pump pathway

Hesperidin exhibits promising potential as a feed additive for augmenting gastric acid secretion in animals. Gastrointestinal function is essential for animal growth and the efficient digestion of dietary nutrients, with gastric acid secretion serving as one of its critical components. The secretion of gastric acid, together with other digestive fluids and substances, significantly influences the digestion and absorption of animal feed, which in turn affects growth performance. However, there is limited research regarding the application of hesperidin as a feed additive to enhance gastric acid secretion. The present study aims to evaluate the efficacy of hesperidin as a feed additive in enhancing gastric acid secretion and to elucidate its underlying mechanisms. A total of 200 newly hatched (1-day-old) broilers with similar body weight were randomly allocated into four groups as follows: the control group receiving only the basal diet, and the other three groups supplemented with 50, 100, and 150 mg of hesperidin per kg of the basal diet, respectively. Each group consisted of five replicates with ten broilers per replicate, and the feeding trial lasted for a duration of 21 days. The growth performance was evaluated by monitoring feed intake and body weight throughout the trial. A four-day nutrient utilization trial was conducted prior to the conclusion of the feeding experiment. Adoption of the total collection method, the collected droppings were weighed and dried at 65 °C. Fifteen broilers from each group were euthanized and immediately dissected to obtain gizzard, proventriculus, gizzard chyme, and jugular blood samples, The proventriculus and gizzard weight were weighed and the pH of gizzard chyme was measured at the same time. The collected jugular venous blood was used to assess gastrin levels, whereas chicken gizzard chyme was utilized for the analysis of lactate, hydrochloric acid, and pepsin activity. Proventriculus and gizzard tissues were used to evaluate pepsinogen levels, perform hematoxylin-eosin (H&E) staining, conduct enzyme-linked immunosorbent assays (ELISAs) for key proton pump components, and assess proton pump activity. The results demonstrated that, in comparison to the control group, both the 100mg/Kg and 150 mg/Kg groups exhibited a significant increase in final body weight (FBW) and average daily gain (ADG) (P < 0.05). Additionally, the feed to gain ratio (F/G) was significantly reduced in the 150mg/Kg group (P < 0.05). The results of the nutrient utilization trial indicate that all treatment groups had significantly higher levels of dry matter (DM) and ether extract (EE) compared to the control group (P < 0.05). Furthermore, crude protein (CP) and gross energy (ME) were significantly higher in the 100mg/Kg and 150mg/Kg groups than in both the control group and the 50mg/Kg group (P < 0.05). The inclusion of hesperidin in broiler diets leads to significant improvements in stomach development and lactic acid content, while pH and hydrochloric acid content exhibit opposite trends (P < 0.05). Supplementation of broiler diets with hesperidin at doses of 100 mg/Kg and 150 mg/Kg significantly up-regulates pepsin activity and pepsinogen levels (P < 0.05). Incorporation of hesperidin into the broilers' diet significantly enhances parietal cell numbers (P < 0.05). Dietary supplementation of hesperidin in broilers effectively up-regulates key signaling pathways and intracellular signal substances involved in proton pump activation (P < 0.05). The proton pump activity also exhibited a significant increase compared to the control group of 100mg/Kg and 150mg/Kg (P < 0.05) in our findings. In conclusion, hesperidin exhibits promising potential as a feed additive for broilers, and it can improve the growth performance of broilers by increasing gastric acid secretion and promoting nutrient utilization through activation of proton pump. Notably, basal diet supplemented with 150mg/Kg hesperidin demonstrates superior efficacy.