光/ ph调节螺吡喃智能响应型亲水分离平台用于肝癌患者血清糖肽的鉴定

IF 6.7 1区化学 Q1 CHEMISTRY, ANALYTICAL

Analytical Chemistry Pub Date : 2025-01-07 DOI:10.1021/acs.analchem.4c04025

Yanqing Zhao, Hongbin Li, Haijiao Zheng, Qiong Jia

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引用次数: 0

摘要

智能响应材料在蛋白质翻译后修饰的富集方面引起了广泛的关注。本文首次基于螺吡喃在光和pH调节下亲水性的变化，开发了一种智能富集策略（MNPs-l-DOPA/PEI-SP），实现了完整糖肽的可控富集和释放。通过理论计算验证了其富集机理和可能的结合机理。基于MNPs-l-DOPA/PEI-SP的智能富集平台用于筛选肝细胞癌（HCC）糖蛋白生物标志物，以评估其癌症诊断和监测性能。在早期HCC患者血清样本中共鉴定出3864个完整的n -糖肽，含166个n -糖蛋白；在正常对照（NC）血清样本中鉴定出3266个完整的n -糖肽，含193个糖蛋白。本研究不仅为利用智能响应材料高效富集完整糖肽提供了新思路，而且为HCC血清液体活检中发现生物标志物开辟了研究可能性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Light-/pH-Regulated Spiropyran Smart-Responsive Hydrophilic Separation Platform for the Identification of Serum Glycopeptides from Hepatocellular Carcinoma Patients

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Light-/pH-Regulated Spiropyran Smart-Responsive Hydrophilic Separation Platform for the Identification of Serum Glycopeptides from Hepatocellular Carcinoma Patients

Smart-responsive materials have attracted much attention in the enrichment of post-translational modifications of proteins. In this work, for the first time, we developed a smart enrichment strategy (MNPs-l-DOPA/PEI-SP) based on the change in hydrophilic properties of spiropyran under the regulation of light and pH to realize the controllable enrichment and release of intact glycopeptides. The enrichment mechanism and possible binding mechanism were verified by theoretical calculations. The smart enrichment platform based on MNPs-l-DOPA/PEI-SP was used to screen glycoprotein biomarkers for hepatocellular carcinoma (HCC) to evaluate its cancer diagnostic and monitoring performance. A total of 3,864 intact N-glycopeptides containing 166 N-glycoproteins were successfully identified in serum samples of early-stage HCC patients, while 3,266 intact N-glycopeptides containing 193 glycoproteins were identified in normal control (NC) serum samples. This work not only provides new ideas for the efficient enrichment of intact glycopeptides with smart-responsive material, but also broadens the research possibilities for biomarker discovery in HCC serum liquid biopsies.

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来源期刊

Analytical Chemistry 化学-分析化学

CiteScore

12.10

自引率

12.20%

发文量

1949

审稿时长

1.4 months

期刊介绍： Analytical Chemistry, a peer-reviewed research journal, focuses on disseminating new and original knowledge across all branches of analytical chemistry. Fundamental articles may explore general principles of chemical measurement science and need not directly address existing or potential analytical methodology. They can be entirely theoretical or report experimental results. Contributions may cover various phases of analytical operations, including sampling, bioanalysis, electrochemistry, mass spectrometry, microscale and nanoscale systems, environmental analysis, separations, spectroscopy, chemical reactions and selectivity, instrumentation, imaging, surface analysis, and data processing. Papers discussing known analytical methods should present a significant, original application of the method, a notable improvement, or results on an important analyte.