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IF 1.6 4区农林科学 Q3 CHEMISTRY, APPLIED

Journal of oleo science Pub Date : 2025-01-01 DOI:10.5650/jos.ess24210

Mudassar Hussain, Sajida Naseem, Asma Abdul Latif, Abid Ali, Muhammad Nauman Aftab, Sikander Ali, Chaman Ara, Irfana Liaqat, Nazish Mazhar Ali, Muhammad Afzaal, Awais Khalid, Guo-Jing Yang, Iram Liaqat

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引用次数: 0

摘要

抗生素耐药性是一个世界性问题，主要发生在发展中国家。本研究评价了后脑假蝇体腔液（PCF）和膏体（PBP）作为病原菌生物膜的抗生物膜和抗群体感应（QS）剂的潜力。提取PCF和PBP，采用结晶紫染色法检测双菌种生物膜形成时间动力学。MH5-MH10)和多物种生物膜(05种组合；MH11-MH15)。采用QS法测定03株铜绿假单胞菌在不同时间段（0、24、48、72、96和120 h）的pyocyanin形成时间动力学。然后，通过测定花青素浓度来分析其抗QS效果。结果表明，在两种细菌生物膜中，MH5-MH7组合在72 h后生物膜含量最高（p < 0.05），而其他3种组合（MH8-MH10）在48 ~ 72 h后生物膜含量最高。同样，对于多物种生物膜，48-72小时后生物膜达到最大值。QS分析显示，PA1菌株在96 h后合成了极显著（p < 0.001）的pyocyanin(20µg/mL)，而PA2和PA3菌株在72 h后合成了极显著（18µg/mL）的pyocyanin。100 ~ 150µg/mL浓度的PCF和PBP对生物膜的抑制效果均显著（p < 0.05），其中200µg/mL浓度的PCF和PBP对所有菌株的抑制效果最高。据我们所知，这是第一次对蚯蚓PCF和PBP的抗菌膜和抗qs潜力的潜在应用进行评估。该研究建议使用精确的生物活性制剂化学方法及其对观察到的干扰的可能作用机制。此外，利用多氯联苯和多氯联苯的生物活性物质合成的新药物肯定具有控制细菌生物膜引起的各种感染问题的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Antibiofilm and Antiquorum Sensing Potential of Pheretima posthum.

Antibiotic resistance is a world wide problem mainly in developing countries. In this work, coelomic fluid (PCF) and paste (PBP) of Pheretima posthuma was assessed for its potential as antibiofilm and anti-quorum sensing (QS) agent against pathogenic bacterial biofilms. PCF and PBP were extracted and biofilm formation time kinetics was examined using crystal violet staining method by utilizing four bacterial isolates in bispecies biofilm (06 combinations; MH5-MH10) and multi species biofilms (05 combinations; MH11-MH15). QS study was performed by determining pyocyanin formation time kinetics using 03 P. aeruginosa strains at various time periods (0, 24, 48, 72, 96 and 120 hours). Following next, anti- QS effect was analyzed by measuring pyocyanin concentrations. Results showed that among bispecies bacterial biofilms, MH5-MH7 combinations showed significantly higher biofilm (p < 0.05) after 72 hours while other three (MH8-MH10) produced maximum biofilm after 48 to 72 hours. Likewise, for multispecies biofilms, maximum biofilm was noted after 48-72 hours. QS analysis revealed that PA1 strain synthesized highly significant (p < 0.001) pyocyanin (20 µg/mL) after 96 hours compared to PA2 and PA3 strains, which formed significant pyocyanin (18 µg/mL) after 72 hours. Also, 100-150 µg/mL of both PCF and PBP exhibited a significant (p < 0.05) reduction in biofilm while 200 µg/mL concentration showed highest QS against all selected strains. To our knowledge, this is the first assessment of its kind on the potential application of earthworm PCF and PBP for its antibiofilm and anti-QS potential. The study recommends precise chemistry of bioactive agents and their probable mechanism(s) of actions for the observed interference. Also, new pharmaceutical drugs synthesized using bioactive agents from PCF and PCB may surely have the potential to manage different infection problems caused by bacterial biofilms.

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来源期刊

Journal of oleo science CHEMISTRY, APPLIED-FOOD SCIENCE & TECHNOLOGY

CiteScore

3.20

自引率

6.70%

发文量

173

审稿时长

3 months

期刊介绍： The J. Oleo Sci. publishes original researches of high quality on chemistry, biochemistry and science of fats and oils such as related food products, detergents, natural products, petroleum products, lipids and related proteins and sugars. The Journal also encourages papers on chemistry and/or biochemistry as a major component combined with biological/ sensory/nutritional/toxicological evaluation related to agriculture and/or food.