液相色谱-单四极杆质谱法同时定量果皮中五环三萜

IF 8.5 1区农林科学 Q1 CHEMISTRY, APPLIED

Food Chemistry Pub Date : 2025-01-05 DOI:10.1016/j.foodchem.2025.142781

Keiji Yabukawa, Ryoichi Izuchi

{"title":"液相色谱-单四极杆质谱法同时定量果皮中五环三萜","authors":"Keiji Yabukawa, Ryoichi Izuchi","doi":"10.1016/j.foodchem.2025.142781","DOIUrl":null,"url":null,"abstract":"The simultaneous determination of pentacyclic triterpenoids, which are secondary metabolites in plants, is difficult because of the similarity in structure and minimal differences in polarity. In this study, we established a simple and sensitive method for the simultaneous determination of pentacyclic triterpenoids based on liquid chromatography–single-quadrupole mass spectrometry (LC-MS) using a C30 column. The established method completely separated 13 pentacyclic triterpenoids based on their different retention times. Furthermore, the molecular weight and reliability were confirmed for ten compounds. This method was used to quantify pentacyclic triterpenoids in acetone extracts of the peels of ten varieties of apple and eight varieties of persimmon. Therefore, this method is expected to be used in a comprehensive search for pentacyclic triterpenoids in fruit-processing residues.","PeriodicalId":318,"journal":{"name":"Food Chemistry","volume":"28 1","pages":""},"PeriodicalIF":8.5000,"publicationDate":"2025-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Simultaneous quantitation of pentacyclic triterpenoids in fruit peels using liquid chromatography–single quadrupole mass spectrometry\",\"authors\":\"Keiji Yabukawa, Ryoichi Izuchi\",\"doi\":\"10.1016/j.foodchem.2025.142781\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The simultaneous determination of pentacyclic triterpenoids, which are secondary metabolites in plants, is difficult because of the similarity in structure and minimal differences in polarity. In this study, we established a simple and sensitive method for the simultaneous determination of pentacyclic triterpenoids based on liquid chromatography–single-quadrupole mass spectrometry (LC-MS) using a C30 column. The established method completely separated 13 pentacyclic triterpenoids based on their different retention times. Furthermore, the molecular weight and reliability were confirmed for ten compounds. This method was used to quantify pentacyclic triterpenoids in acetone extracts of the peels of ten varieties of apple and eight varieties of persimmon. Therefore, this method is expected to be used in a comprehensive search for pentacyclic triterpenoids in fruit-processing residues.\",\"PeriodicalId\":318,\"journal\":{\"name\":\"Food Chemistry\",\"volume\":\"28 1\",\"pages\":\"\"},\"PeriodicalIF\":8.5000,\"publicationDate\":\"2025-01-05\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Food Chemistry\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1016/j.foodchem.2025.142781\",\"RegionNum\":1,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"CHEMISTRY, APPLIED\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Food Chemistry","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1016/j.foodchem.2025.142781","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, APPLIED","Score":null,"Total":0}

引用次数: 0

摘要

五环三萜是植物次生代谢产物，由于结构相似，极性差异极小，因此很难同时测定。本研究采用C30色谱柱，建立了一种简便、灵敏的液相色谱-单四极杆质谱（LC-MS）同时测定五环三萜的方法。该方法根据保留时间的不同，完全分离了13种五环三萜。此外，还确定了10个化合物的分子量和可靠性。用该方法定量测定了10个苹果品种和8个柿子品种果皮丙酮提取物中的五环三萜。因此，该方法有望用于水果加工残留物中五环三萜的综合搜索。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Simultaneous quantitation of pentacyclic triterpenoids in fruit peels using liquid chromatography–single quadrupole mass spectrometry

查看原文本刊更多论文

Simultaneous quantitation of pentacyclic triterpenoids in fruit peels using liquid chromatography–single quadrupole mass spectrometry

The simultaneous determination of pentacyclic triterpenoids, which are secondary metabolites in plants, is difficult because of the similarity in structure and minimal differences in polarity. In this study, we established a simple and sensitive method for the simultaneous determination of pentacyclic triterpenoids based on liquid chromatography–single-quadrupole mass spectrometry (LC-MS) using a C30 column. The established method completely separated 13 pentacyclic triterpenoids based on their different retention times. Furthermore, the molecular weight and reliability were confirmed for ten compounds. This method was used to quantify pentacyclic triterpenoids in acetone extracts of the peels of ten varieties of apple and eight varieties of persimmon. Therefore, this method is expected to be used in a comprehensive search for pentacyclic triterpenoids in fruit-processing residues.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Food Chemistry 工程技术-食品科技

CiteScore

16.30

自引率

10.20%

发文量

3130

审稿时长

122 days

期刊介绍： Food Chemistry publishes original research papers dealing with the advancement of the chemistry and biochemistry of foods or the analytical methods/ approach used. All papers should focus on the novelty of the research carried out.