揭示干旱胁迫下菠菜植物的生长和代谢动力学：探索生物赤霉素的贡献

IF 3.9 2区农林科学 Q1 HORTICULTURE

Scientia Horticulturae Pub Date : 2025-01-15 DOI:10.1016/j.scienta.2024.113924

Hend A. Hamed , Ghada Abd-Elmonsef Mahmoud , Amany H.A. Abeed

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引用次数: 0

摘要

可持续农业是粮食及农业组织（粮农组织）《2022-2031年战略框架》的一个重要主题。使用基于微生物的调节剂是实现食品安全的可持续有机方法。通常情况下，干旱对大多数农作物的农业生产都是一个威胁，但对于绿叶蔬菜来说，由于等级标准的限制，这一问题更令人沮丧。尽管在提高作物对干旱的耐受性方面取得了突破，但对绿叶蔬菜的追求仍然受到限制。本报告旨在研究尖孢镰刀菌（Fusarium oxysporum）生物产生的赤霉素（gibberellin）缓解叶菜菠菜（Spinacia oleracea）水分胁迫的能力。内生真菌尖孢镰刀菌（Fusarium oxysporum）的赤霉素产量高达200±5.9 mg L−1。水分胁迫（100,75,50,25%田间容量，FC）产生轻度至重度生长异常和生理动态。叶面施用生物赤霉素（BG）通过提高植株高度、生物量和叶片数量等各种表型和生理特征，并伴随着更厚的表皮蜡、平衡的水分状态、更高的光合色素和增加的渗透保护剂，来促进植物的产量和质量。BG通过增强抗氧化剂（花青素、抗坏血酸、总抗氧化剂和类黄酮），激活次级代谢酶苯丙氨酸解氨酶PAL，满足消费者对菠菜的需求标准，降低酚类物质及其氧化酶多酚氧化酶（PPO，褐变原因）的含量，从而提高植物的活性。过氧化氢酶（CAT）、超氧化物歧化酶（SOD）和过氧化物酶（POD）被激活，从而使电解质泄漏、细胞O2·−、·OH、H2O2、丙二醛和脂质过氧化维持在基线水平。BG似乎通过提高硝酸还原酶（NR）活性来降低硝酸盐毒性。BG叶面喷雾剂增加了菠菜对脱水的恢复能力，并在缺水情况下甚至暴露于干旱条件下生产足够的升级产量的能力。

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Unraveling growth and metabolic dynamics in drought-stressed spinach plants: Exploring the contribution of biological gibberellin

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Unraveling growth and metabolic dynamics in drought-stressed spinach plants: Exploring the contribution of biological gibberellin

Sustainable agriculture is a major theme of the Food and Agriculture Organization (FAO) Strategic Framework 2022–2031. Using microbial-based regulators is a sustainable organic approach to accomplish food safety. Normally, drought is a menace to most crops' agricultural production, but for leafy green vegetables the matter is more frustrating due to grade standards. Despite breakthroughs in boosting crop tolerance to drought stress, the quest for leafy greens remains restricted. The current report is to study the ability of biologically-produced gibberellin by Fusarium oxysporum in alleviating water stress in leafy vegetable spinach, Spinacia oleracea. Endophytic Fusarium oxysporum demonstrated high gibberellin production by 200±5.9 mg L⁻¹. Water stress (100, 75, 50, 25 % field capacity, FC) generated mild to severe abnormal growth and physiological dynamics. Foliar-applied biological gibberellin (BG) motivated plant yield and quality by boosting various phenotypic and physiological features in terms of plant height, biomass, and number of leaves accompanied by thicker epicuticular wax, balanced water status, higher photosynthetic pigment, increased osmoprotectants. BG shoulders a role in upgrading plant liveness via exacerbating antioxidants (anthocyanin, ascorbic acids, total antioxidants, and flavonoids) joined with activation of secondary metabolizing enzyme phenylalanine ammonia-lyase PAL, fulfilling consumer demand standards for spinach as well as lowering the content of phenolics and its oxidizing enzyme polyphenol oxidas (PPO, browning causer). Catalase (CAT), superoxide dismutase (SOD), and peroxidase (POD) were instigated thus maintaining electrolyte leakage, cellular O₂^·−, ^·OH, H₂O₂, malondialdehyde, and lipid peroxidation at baseline levels. BG appears to reduce nitrative toxicity via enhancing nitrate reductase (NR) activity. BG foliar spray increased spinach's resilience to dehydration and its capacity to produce an adequate upgraded yield while cultivated with reduced water regimes or even exposed to drought.

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来源期刊

Scientia Horticulturae 农林科学-园艺

CiteScore

8.60

自引率

4.70%

发文量

796

审稿时长

47 days

期刊介绍： Scientia Horticulturae is an international journal publishing research related to horticultural crops. Articles in the journal deal with open or protected production of vegetables, fruits, edible fungi and ornamentals under temperate, subtropical and tropical conditions. Papers in related areas (biochemistry, micropropagation, soil science, plant breeding, plant physiology, phytopathology, etc.) are considered, if they contain information of direct significance to horticulture. Papers on the technical aspects of horticulture (engineering, crop processing, storage, transport etc.) are accepted for publication only if they relate directly to the living product. In the case of plantation crops, those yielding a product that may be used fresh (e.g. tropical vegetables, citrus, bananas, and other fruits) will be considered, while those papers describing the processing of the product (e.g. rubber, tobacco, and quinine) will not. The scope of the journal includes all horticultural crops but does not include speciality crops such as, medicinal crops or forestry crops, such as bamboo. Basic molecular studies without any direct application in horticulture will not be considered for this journal.