Jia Hao Xie, Hai Yan Xing, Li Meng, Yong Zhao, Qiang Zeng, Qi Xiao, Nian Bing Li, Peng Xue, Hong Qun Luo
{"title":"通过诱导分子聚集抑制TICT形成的工程HClO比率荧光探针监测药物性肝损伤","authors":"Jia Hao Xie, Hai Yan Xing, Li Meng, Yong Zhao, Qiang Zeng, Qi Xiao, Nian Bing Li, Peng Xue, Hong Qun Luo","doi":"10.1021/acs.analchem.4c03839","DOIUrl":null,"url":null,"abstract":"Developing highly specific hypochlorite (HClO) ratiometric fluorescent probes for elucidating drug-induced liver injury (DILI) and related biological issues is of great significance. Phenothiazine-derived coumarin (PTZC) fluorophores have been shown to exhibit high specificity in response to HClO; however, such fluorophores emit dim fluorescence in aqueous media due to the intrinsic twisted intramolecular charge transfer (TICT) effect, resulting in only a turn-on mode response to HClO. In this work, we present a molecular design strategy of suppressing the TICT formation by inducing PTZC fluorophores to form nanoaggregates in aqueous media for the HClO ratiometric fluorescent probe design. The probe <b>PBFF</b> developed based on this strategy can form considerable nanoaggregates in aqueous media to inhibit the TICT formation without suffering from the aggregation-caused quenching effect, thus allowing it to emit intense fluorescence in aqueous media (Φ<sub><i>f</i></sub> = 0.20). Importantly, <b>PBFF</b> can specifically and sensitively (LOD = 15.7 nM) respond to HClO, showing distinct ratiometric fluorescence changes. Further experimental results show that <b>PBFF</b> can sensitively monitor in situ DILI progression through HClO-induced fluorescence ratio changes. This strategy of suppressing TICT formation by inducing molecule aggregation in aqueous media will advance the development of ratiometric fluorescent probes in the future.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"15 1","pages":""},"PeriodicalIF":6.7000,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Engineering HClO Ratiometric Fluorescent Probe by Inducing Molecular Aggregation to Suppress TICT Formation for Monitoring Drug-Induced Liver Injury\",\"authors\":\"Jia Hao Xie, Hai Yan Xing, Li Meng, Yong Zhao, Qiang Zeng, Qi Xiao, Nian Bing Li, Peng Xue, Hong Qun Luo\",\"doi\":\"10.1021/acs.analchem.4c03839\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Developing highly specific hypochlorite (HClO) ratiometric fluorescent probes for elucidating drug-induced liver injury (DILI) and related biological issues is of great significance. Phenothiazine-derived coumarin (PTZC) fluorophores have been shown to exhibit high specificity in response to HClO; however, such fluorophores emit dim fluorescence in aqueous media due to the intrinsic twisted intramolecular charge transfer (TICT) effect, resulting in only a turn-on mode response to HClO. In this work, we present a molecular design strategy of suppressing the TICT formation by inducing PTZC fluorophores to form nanoaggregates in aqueous media for the HClO ratiometric fluorescent probe design. The probe <b>PBFF</b> developed based on this strategy can form considerable nanoaggregates in aqueous media to inhibit the TICT formation without suffering from the aggregation-caused quenching effect, thus allowing it to emit intense fluorescence in aqueous media (Φ<sub><i>f</i></sub> = 0.20). Importantly, <b>PBFF</b> can specifically and sensitively (LOD = 15.7 nM) respond to HClO, showing distinct ratiometric fluorescence changes. Further experimental results show that <b>PBFF</b> can sensitively monitor in situ DILI progression through HClO-induced fluorescence ratio changes. This strategy of suppressing TICT formation by inducing molecule aggregation in aqueous media will advance the development of ratiometric fluorescent probes in the future.\",\"PeriodicalId\":27,\"journal\":{\"name\":\"Analytical Chemistry\",\"volume\":\"15 1\",\"pages\":\"\"},\"PeriodicalIF\":6.7000,\"publicationDate\":\"2024-12-31\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Analytical Chemistry\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://doi.org/10.1021/acs.analchem.4c03839\",\"RegionNum\":1,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"CHEMISTRY, ANALYTICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Analytical Chemistry","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1021/acs.analchem.4c03839","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
Engineering HClO Ratiometric Fluorescent Probe by Inducing Molecular Aggregation to Suppress TICT Formation for Monitoring Drug-Induced Liver Injury
Developing highly specific hypochlorite (HClO) ratiometric fluorescent probes for elucidating drug-induced liver injury (DILI) and related biological issues is of great significance. Phenothiazine-derived coumarin (PTZC) fluorophores have been shown to exhibit high specificity in response to HClO; however, such fluorophores emit dim fluorescence in aqueous media due to the intrinsic twisted intramolecular charge transfer (TICT) effect, resulting in only a turn-on mode response to HClO. In this work, we present a molecular design strategy of suppressing the TICT formation by inducing PTZC fluorophores to form nanoaggregates in aqueous media for the HClO ratiometric fluorescent probe design. The probe PBFF developed based on this strategy can form considerable nanoaggregates in aqueous media to inhibit the TICT formation without suffering from the aggregation-caused quenching effect, thus allowing it to emit intense fluorescence in aqueous media (Φf = 0.20). Importantly, PBFF can specifically and sensitively (LOD = 15.7 nM) respond to HClO, showing distinct ratiometric fluorescence changes. Further experimental results show that PBFF can sensitively monitor in situ DILI progression through HClO-induced fluorescence ratio changes. This strategy of suppressing TICT formation by inducing molecule aggregation in aqueous media will advance the development of ratiometric fluorescent probes in the future.
期刊介绍:
Analytical Chemistry, a peer-reviewed research journal, focuses on disseminating new and original knowledge across all branches of analytical chemistry. Fundamental articles may explore general principles of chemical measurement science and need not directly address existing or potential analytical methodology. They can be entirely theoretical or report experimental results. Contributions may cover various phases of analytical operations, including sampling, bioanalysis, electrochemistry, mass spectrometry, microscale and nanoscale systems, environmental analysis, separations, spectroscopy, chemical reactions and selectivity, instrumentation, imaging, surface analysis, and data processing. Papers discussing known analytical methods should present a significant, original application of the method, a notable improvement, or results on an important analyte.