具有双通道电化学检测的3d打印双通道流动小型化设备

IF 6.7 1区化学 Q1 CHEMISTRY, ANALYTICAL

Analytical Chemistry Pub Date : 2024-12-24 DOI:10.1021/acs.analchem.4c04099

Miriam Chávez, Alberto Escarpa

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引用次数: 0

摘要

在这里，我们提出了具有双电化学检测（EDd）的三维打印双通道流动小型化装置（3Dd），该装置将两个工作电极分别集成在通道内配置（3Dd-EDd）中。采用普鲁士蓝（PB）壳金纳米粒子（PB）AuNP)核基电化学法选择性测定过氧化氢。3Dd-EDd器件表现出良好的稳定性，通道内和通道间电化学性能一致，器件间精度低于9%，显示了器件设计和制造的可靠性。3Dd-EDd能够在促氧化刺激下长期可靠地测定Caco-2细胞生理pH下的过氧化氢，证明了其出色的电分析性能。研究结果强调了基于3d打印的小型设备的分析通用性和可靠性，该设备集成了先进的电化学技术及其实时细胞监测的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

3D-Printed Dual-Channel Flow-Through Miniaturized Devices with Dual In-Channel Electrochemical Detection

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3D-Printed Dual-Channel Flow-Through Miniaturized Devices with Dual In-Channel Electrochemical Detection

Here, we present three-dimensional-printed dual-channel flow-through miniaturized devices (3D_d) with dual electrochemical detection (ED_d) integrating two working electrodes each in an in-channel configuration (3D_d-ED_d). Prussian Blue (PB) shell-gold nanoparticles ((PB)AuNP) core-based electrochemistry was chosen for selective hydrogen peroxide determination. 3D_d-ED_d devices exhibited impress stability, identical intrachannel and interchannel electrochemical performances, and excellent interdevice precision with values under 9%, revealing the reliability of the design and fabrication of the devices. 3D_d-ED_d enabled long-term reliable hydrogen peroxide determination at physiological pH in Caco-2 cells under prooxidant stimulation demonstrating its outstanding electroanalytical performance. The results highlight the analytical versatility and trustworthiness of 3D-printing-based devices at miniaturized scale integrating advanced electrochemistry and its potential for real-time cell monitoring.

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来源期刊

Analytical Chemistry 化学-分析化学

CiteScore

12.10

自引率

12.20%

发文量

1949

审稿时长

1.4 months

期刊介绍： Analytical Chemistry, a peer-reviewed research journal, focuses on disseminating new and original knowledge across all branches of analytical chemistry. Fundamental articles may explore general principles of chemical measurement science and need not directly address existing or potential analytical methodology. They can be entirely theoretical or report experimental results. Contributions may cover various phases of analytical operations, including sampling, bioanalysis, electrochemistry, mass spectrometry, microscale and nanoscale systems, environmental analysis, separations, spectroscopy, chemical reactions and selectivity, instrumentation, imaging, surface analysis, and data processing. Papers discussing known analytical methods should present a significant, original application of the method, a notable improvement, or results on an important analyte.