{"title":"转录组和miRNAome分析揭示了miR6155在烟草毛状体发育中的调节作用","authors":"Mengxiao Xu, Jianfeng Zhang, Lijun Meng, Huan Su, Zechao Qu, Jiemeng Tao, Peng Lu, Deshui Liu, Shizhou Yu, Chenfeng Hua, Jinbang Wang, Peijian Cao, Jingjing Jin","doi":"10.1186/s40538-024-00718-2","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><p>Glandular trichomes, which act as the first barrier against damage induced by insects and disease, can produce specialized metabolites that play important roles during plant development. However, the role played by microRNAs (miRNAs), which regulate many plant physiological processes, during trichome development is not unraveled in detail.</p><h3>Results</h3><p>In this study, we performed RNA sequencing (RNA-Seq) and small RNA sequencing assessments of tobacco trichome, leaf minus trichome (leaf-trichome), and leaf tissue to improve our understanding of the miRNA mechanisms regulating trichome development. Totally, we identified 270 differentially expressed miRNAs (DEMs) and 10,430 differentially expressed genes (DEGs) between trichome and leaf-trichome tissues. DEM targets were mainly associated with plant hormone signal transduction, plant–pathogen interactions, and the biosynthesis of secondary metabolites. Of these, 1233 miRNA–mRNA pairs were identified with reverse expression patterns. Next, we used dual-luciferase reporter (LUC) assays to reveal that several potential targets were significantly inhibited by corresponding miRNAs, including the transcription factors (TF) <i>NAC021</i>, <i>AP2</i>, <i>MYB36</i>, <i>WRKY6</i> and <i>TIFY10B</i>. Further analysis showed that miR6155-WRKY6 might perform vital roles in trichome development, and that overexpression of miR6155 resulted in decreased trichome density.</p><h3>Conclusions</h3><p>Taken together, these findings demonstrate that miRNAs may be involved in trichome development in tobacco, and they may advance our understanding of the regulation of trichome development mediated by miRNA and can help to improve genetic engineering of trichome regulation in plants.</p><h3>Graphical Abstract</h3>\n<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":512,"journal":{"name":"Chemical and Biological Technologies in Agriculture","volume":"11 1","pages":""},"PeriodicalIF":5.2000,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://chembioagro.springeropen.com/counter/pdf/10.1186/s40538-024-00718-2","citationCount":"0","resultStr":"{\"title\":\"Transcriptome and miRNAome analyses uncover the regulatory role of miR6155 in trichome development of tobacco\",\"authors\":\"Mengxiao Xu, Jianfeng Zhang, Lijun Meng, Huan Su, Zechao Qu, Jiemeng Tao, Peng Lu, Deshui Liu, Shizhou Yu, Chenfeng Hua, Jinbang Wang, Peijian Cao, Jingjing Jin\",\"doi\":\"10.1186/s40538-024-00718-2\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Background</h3><p>Glandular trichomes, which act as the first barrier against damage induced by insects and disease, can produce specialized metabolites that play important roles during plant development. However, the role played by microRNAs (miRNAs), which regulate many plant physiological processes, during trichome development is not unraveled in detail.</p><h3>Results</h3><p>In this study, we performed RNA sequencing (RNA-Seq) and small RNA sequencing assessments of tobacco trichome, leaf minus trichome (leaf-trichome), and leaf tissue to improve our understanding of the miRNA mechanisms regulating trichome development. Totally, we identified 270 differentially expressed miRNAs (DEMs) and 10,430 differentially expressed genes (DEGs) between trichome and leaf-trichome tissues. DEM targets were mainly associated with plant hormone signal transduction, plant–pathogen interactions, and the biosynthesis of secondary metabolites. Of these, 1233 miRNA–mRNA pairs were identified with reverse expression patterns. Next, we used dual-luciferase reporter (LUC) assays to reveal that several potential targets were significantly inhibited by corresponding miRNAs, including the transcription factors (TF) <i>NAC021</i>, <i>AP2</i>, <i>MYB36</i>, <i>WRKY6</i> and <i>TIFY10B</i>. Further analysis showed that miR6155-WRKY6 might perform vital roles in trichome development, and that overexpression of miR6155 resulted in decreased trichome density.</p><h3>Conclusions</h3><p>Taken together, these findings demonstrate that miRNAs may be involved in trichome development in tobacco, and they may advance our understanding of the regulation of trichome development mediated by miRNA and can help to improve genetic engineering of trichome regulation in plants.</p><h3>Graphical Abstract</h3>\\n<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>\",\"PeriodicalId\":512,\"journal\":{\"name\":\"Chemical and Biological Technologies in Agriculture\",\"volume\":\"11 1\",\"pages\":\"\"},\"PeriodicalIF\":5.2000,\"publicationDate\":\"2024-12-20\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://chembioagro.springeropen.com/counter/pdf/10.1186/s40538-024-00718-2\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Chemical and Biological Technologies in Agriculture\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://link.springer.com/article/10.1186/s40538-024-00718-2\",\"RegionNum\":2,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"AGRICULTURE, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Chemical and Biological Technologies in Agriculture","FirstCategoryId":"97","ListUrlMain":"https://link.springer.com/article/10.1186/s40538-024-00718-2","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRICULTURE, MULTIDISCIPLINARY","Score":null,"Total":0}
Transcriptome and miRNAome analyses uncover the regulatory role of miR6155 in trichome development of tobacco
Background
Glandular trichomes, which act as the first barrier against damage induced by insects and disease, can produce specialized metabolites that play important roles during plant development. However, the role played by microRNAs (miRNAs), which regulate many plant physiological processes, during trichome development is not unraveled in detail.
Results
In this study, we performed RNA sequencing (RNA-Seq) and small RNA sequencing assessments of tobacco trichome, leaf minus trichome (leaf-trichome), and leaf tissue to improve our understanding of the miRNA mechanisms regulating trichome development. Totally, we identified 270 differentially expressed miRNAs (DEMs) and 10,430 differentially expressed genes (DEGs) between trichome and leaf-trichome tissues. DEM targets were mainly associated with plant hormone signal transduction, plant–pathogen interactions, and the biosynthesis of secondary metabolites. Of these, 1233 miRNA–mRNA pairs were identified with reverse expression patterns. Next, we used dual-luciferase reporter (LUC) assays to reveal that several potential targets were significantly inhibited by corresponding miRNAs, including the transcription factors (TF) NAC021, AP2, MYB36, WRKY6 and TIFY10B. Further analysis showed that miR6155-WRKY6 might perform vital roles in trichome development, and that overexpression of miR6155 resulted in decreased trichome density.
Conclusions
Taken together, these findings demonstrate that miRNAs may be involved in trichome development in tobacco, and they may advance our understanding of the regulation of trichome development mediated by miRNA and can help to improve genetic engineering of trichome regulation in plants.
期刊介绍：
Chemical and Biological Technologies in Agriculture is an international, interdisciplinary, peer-reviewed forum for the advancement and application to all fields of agriculture of modern chemical, biochemical and molecular technologies. The scope of this journal includes chemical and biochemical processes aimed to increase sustainable agricultural and food production, the evaluation of quality and origin of raw primary products and their transformation into foods and chemicals, as well as environmental monitoring and remediation. Of special interest are the effects of chemical and biochemical technologies, also at the nano and supramolecular scale, on the relationships between soil, plants, microorganisms and their environment, with the help of modern bioinformatics. Another special focus is the use of modern bioorganic and biological chemistry to develop new technologies for plant nutrition and bio-stimulation, advancement of biorefineries from biomasses, safe and traceable food products, carbon storage in soil and plants and restoration of contaminated soils to agriculture.
This journal presents the first opportunity to bring together researchers from a wide number of disciplines within the agricultural chemical and biological sciences, from both industry and academia. The principle aim of Chemical and Biological Technologies in Agriculture is to allow the exchange of the most advanced chemical and biochemical knowledge to develop technologies which address one of the most pressing challenges of our times - sustaining a growing world population.
Chemical and Biological Technologies in Agriculture publishes original research articles, short letters and invited reviews. Articles from scientists in industry, academia as well as private research institutes, non-governmental and environmental organizations are encouraged.
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