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IF 5.2 2区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY

Chemical and Biological Technologies in Agriculture Pub Date : 2024-12-18 DOI:10.1186/s40538-024-00712-8

Yanhui Feng, Baoyu Tian, Juan Xiong, Guoqin Lin, Linjie Cheng, Ting Zhang, Bilian Lin, Zhenhua Ke, Xin Li

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引用次数: 0

摘要

探索植物生长促进细菌的吲哚乙酸（IAA）生物合成途径及其合成 IAA 的能力，对于了解促进机制和开发更高效的微生物肥料至关重要。本研究从番茄根部分离了 118 株内生细菌，其中 68 株被鉴定为芽孢杆菌属和假单胞菌属。在对这些鉴定出的芽孢杆菌和假单胞菌进行体外合成 IAA 和促进植物生长的能力筛选后，7 株内生菌株 Bacillus sp. Y_21、B. paramycoides Y_29、B. albus Y_96、B. cereus Y_121、P. plecoglossicida Y_157、Bacillus sp.Y_165 和 B. aryabhattai Y_170 菌株对小麦根系或叶片有显著的促进作用，其中 4 个内生菌株具有利用色胺和吲哚乙酰胺等中间代谢产物产生 IAA 的潜在能力。对选定的具有代表性的植物生长促进内生菌进行基因组测序分析表明，产生IAA的细菌B. cereus mr31和P. putida Y_166分别具有完整的吲哚乙酰胺（IAM）和完整的色胺（TAM）途径；而Bacillus sp. Y_165和B. aryabhattai Y_170这两株不利用色胺而利用吲哚-3-乙酰胺产生IAA的菌株则具有不完整的IAM途径。三株基因组序列菌株以小麦或番茄根提取物为底物进行发酵实验，并结合小麦根提取物的超高效液相色谱-质谱/质谱分析，证明芽孢杆菌 Y_165 株可能利用植物来源的吲哚-3-乙酰胺产生 IAA。我们的研究首次证明了植物生长促进内生菌利用植物源中间产物生物合成 IAA 的一种新的潜在机制。这一额外的机制加深了我们对细菌内生菌如何促进植物健康和恢复能力的理解，为植物如何与细菌内生菌共同调节其内部组织的IAA平衡提供了宝贵的见解。

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Exploring IAA biosynthesis and plant growth promotion mechanism for tomato root endophytes with incomplete IAA synthesis pathways

Exploring indoleacetic acid (IAA) biosynthesis pathways of plant growth promoting bacteria and their ability to synthesize IAA is crucial for understanding the promotion mechanism and for developing more efficient microbial fertilizer. In this study, 118 bacterial endophytic strains were isolated from tomato root and 68 isolates were identified as members of Bacillus and Pseudomonas genus. After screening abilities to synthesize IAA in vitro and promote plant growth for these identified Bacillus and Pseudomonas strains, 7 endophytic strains Bacillus sp. Y_21, B. paramycoides Y_29, B. albus Y_96, B. cereus Y_121, P. plecoglossicida Y_157, Bacillus sp. Y_165 and B. aryabhattai Y_170 strains showed a significant promotion role on wheat root or leaf, including 4 endophytic strains with the potential ability to utilize intermediate metabolites, such as tryptamine and indole acetamide, to produce IAA. Genomic sequencing analysis for selected representative plant growth promoting endophytes showed that IAA-producing bacteria B. cereus mr31 and P. putida Y_166 separately harbored a complete indoleacetamide (IAM) and complete tryptamine (TAM) pathway; whereas, Bacillus sp. Y_165 and B. aryabhattai Y_170, two strains producing IAA not using tryptophan but with indole-3-acetamide, had an incomplete IAM pathway. Fermentation experiments of three genome-sequenced strains using wheat or tomato root extracts as substrate, and combining with UHPLC–MS/MS analysis for wheat root extracts, demonstrated that Bacillus sp. Y_165 strain might produce IAA by using the plant-derived indole-3-acetamide. Our study first demonstrated a novel potential mechanism for the plant growth promoting endophytes to biosynthesize IAA using plant-derived intermediates. This additional mechanism has deepened our understanding of how bacterial endophytes promote plant health and resilience, offering valuable insights about how plants regulate IAA homeostasis within their internal tissues in association with bacterial endophytes.

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来源期刊

Chemical and Biological Technologies in Agriculture Biochemistry, Genetics and Molecular Biology-Biotechnology

CiteScore

6.80

自引率

3.00%

发文量

审稿时长

15 weeks

期刊介绍： Chemical and Biological Technologies in Agriculture is an international, interdisciplinary, peer-reviewed forum for the advancement and application to all fields of agriculture of modern chemical, biochemical and molecular technologies. The scope of this journal includes chemical and biochemical processes aimed to increase sustainable agricultural and food production, the evaluation of quality and origin of raw primary products and their transformation into foods and chemicals, as well as environmental monitoring and remediation. Of special interest are the effects of chemical and biochemical technologies, also at the nano and supramolecular scale, on the relationships between soil, plants, microorganisms and their environment, with the help of modern bioinformatics. Another special focus is the use of modern bioorganic and biological chemistry to develop new technologies for plant nutrition and bio-stimulation, advancement of biorefineries from biomasses, safe and traceable food products, carbon storage in soil and plants and restoration of contaminated soils to agriculture. This journal presents the first opportunity to bring together researchers from a wide number of disciplines within the agricultural chemical and biological sciences, from both industry and academia. The principle aim of Chemical and Biological Technologies in Agriculture is to allow the exchange of the most advanced chemical and biochemical knowledge to develop technologies which address one of the most pressing challenges of our times - sustaining a growing world population. Chemical and Biological Technologies in Agriculture publishes original research articles, short letters and invited reviews. Articles from scientists in industry, academia as well as private research institutes, non-governmental and environmental organizations are encouraged.