缺氧期间分枝杆菌 MraZ 对 dcw 操作子内外的多个基因进行调控

IF 3.8 2区 医学 Q2 CHEMISTRY, MEDICINAL
Ismail Mohamed Suleiman, Huang Yu, Junqi Xu, Junfeng Zhen, Hongxiang Xu, Abulimiti Abudukadier, Amina Rafique Hafiza and Jianping Xie*, 
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引用次数: 0

摘要

结核分枝杆菌是最古老的人类结核病病原体,在人类历史上一直是细菌性传染病导致死亡的主要原因。根据世界卫生组织《全球结核病报告》,2022年新增结核病病例750万例,是世界卫生组织1995年启动全球结核病监测项目以来的最高病例数。2019年的高峰为710万例,2020年为580万例,2021年为640万例。2022年的增加可能是由于COVID-19大流行使结核病病例追踪复杂化,这引起了人们的关注。为了更好地了解耻垢分枝杆菌mraZ在缺氧条件下的调控谱,我们使用Illumina Agilent 5300测序对耻垢分枝杆菌突变株和野生型菌株进行了转录组分析。该研究鉴定了6898个差异表达基因,这些基因用NCBI非冗余蛋白序列、人工注释和审查的蛋白质序列数据库、Pfam、Clusters of Orthologous Groups of Proteins、Gene Ontology和Kyoto Encyclopedia of genes and Genomes进行了注释。对分枝杆菌转录调控因子、毒力基因、膜转运蛋白和细胞壁生物合成基因进行了注释。这些数据为未来的调查提供了宝贵的资源,并可能为开发抗结核分枝杆菌感染的药物提供见解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Mycobacterium smegmatis MraZ Regulates Multiple Genes within and Outside of the dcw Operon during Hypoxia

Mycobacterium smegmatis MraZ Regulates Multiple Genes within and Outside of the dcw Operon during Hypoxia

Mycobacterium tuberculosis is the most ancient human tuberculosis pathogen and has been the leading cause of death from bacterial infectious diseases throughout human history. According to the World Health Organization Global Tuberculosis Report, in 2022, 7.5 million new tuberculosis cases were identified, marking the highest number of cases since the World Health Organization initiated its worldwide tuberculosis surveillance program in 1995. The 2019 peak was 7.1 million cases, with 5.8 million cases in 2020 and 6.4 million in 2021. The increase in 2022, which may be attributed to the COVID-19 pandemic complicating tuberculosis case tracing, has raised concerns. To better understand the regulation spectrum of Mycobacterium smegmatis mraZ under hypoxia, we performed a transcriptome analysis of M. smegmatis mutant and wild-type strains using Illumina Agilent 5300 sequencing. The study identified 6898 differentially expressed genes, which were annotated with NCBI nonredundant protein sequences, a manually annotated and reviewed protein sequence database, Pfam, Clusters of Orthologous Groups of Proteins, Gene Ontology, and Kyoto Encyclopedia of Genes and Genomes. Several mycobacteria transcriptional regulators, virulence genes, membrane transporters, and cell wall biosynthesis genes were annotated. These data serve as a valuable resource for future investigations and may offer insight into the development of drugs to combat M. tuberculosis infection.

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来源期刊
ACS Infectious Diseases
ACS Infectious Diseases CHEMISTRY, MEDICINALINFECTIOUS DISEASES&nb-INFECTIOUS DISEASES
CiteScore
9.70
自引率
3.80%
发文量
213
期刊介绍: ACS Infectious Diseases will be the first journal to highlight chemistry and its role in this multidisciplinary and collaborative research area. The journal will cover a diverse array of topics including, but not limited to: * Discovery and development of new antimicrobial agents — identified through target- or phenotypic-based approaches as well as compounds that induce synergy with antimicrobials. * Characterization and validation of drug target or pathways — use of single target and genome-wide knockdown and knockouts, biochemical studies, structural biology, new technologies to facilitate characterization and prioritization of potential drug targets. * Mechanism of drug resistance — fundamental research that advances our understanding of resistance; strategies to prevent resistance. * Mechanisms of action — use of genetic, metabolomic, and activity- and affinity-based protein profiling to elucidate the mechanism of action of clinical and experimental antimicrobial agents. * Host-pathogen interactions — tools for studying host-pathogen interactions, cellular biochemistry of hosts and pathogens, and molecular interactions of pathogens with host microbiota. * Small molecule vaccine adjuvants for infectious disease. * Viral and bacterial biochemistry and molecular biology.
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