链霉菌LysR转录调控因子在环脂肽octaminomycin C和D中的应用

IF 3.3 2区生物学 Q2 CHEMISTRY, MEDICINAL

Journal of Natural Products Pub Date : 2024-12-27 Epub Date: 2024-12-13 DOI:10.1021/acs.jnatprod.4c01047

Joon Soo An, Jung Min Kim, Jaeho Han, Jun Young Lee, Sang-Jip Nam, Sang Kook Lee, Yeo Joon Yoon, Dong-Chan Oh

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引用次数: 0

摘要

在一株过表达LysR转录调节因子家族的链霉菌中发现了新的脂沉肽，octaminomycin C和D（1和2）。1和2的结构通过紫外、HRMS和NMR的综合分析得到了证实。绝对构型是通过光谱分析、先进的Marfey方法和基因组分析确定的。基因组分析鉴定了辛胺菌素的非核糖体肽合成酶（NRPS）生物合成途径。

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Discovery of Cyclic Lipopeptides, Octaminomycins C and D, by Engineering LysR Transcriptional Regulator of Streptomyces sp.

New lipodepsipeptides, octaminomycins C and D (1 and 2), were discovered in an engineered Streptomyces sp. strain overexpressing the LysR transcriptional regulator family. The structures of 1 and 2 were elucidated by comprehensive analysis of their UV, HRMS, and NMR data. The absolute configurations were determined using spectroscopic analysis, the advanced Marfey's method, and genomic analysis. Genomic analysis enabled the identification of the nonribosomal peptide synthetase (NRPS) biosynthetic pathway of the octaminomycins.

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来源期刊

Journal of Natural Products 生物-药学

CiteScore

9.10

自引率

5.90%

发文量

294

审稿时长

2.3 months

期刊介绍： The Journal of Natural Products invites and publishes papers that make substantial and scholarly contributions to the area of natural products research. Contributions may relate to the chemistry and/or biochemistry of naturally occurring compounds or the biology of living systems from which they are obtained. Specifically, there may be articles that describe secondary metabolites of microorganisms, including antibiotics and mycotoxins; physiologically active compounds from terrestrial and marine plants and animals; biochemical studies, including biosynthesis and microbiological transformations; fermentation and plant tissue culture; the isolation, structure elucidation, and chemical synthesis of novel compounds from nature; and the pharmacology of compounds of natural origin. When new compounds are reported, manuscripts describing their biological activity are much preferred. Specifically, there may be articles that describe secondary metabolites of microorganisms, including antibiotics and mycotoxins; physiologically active compounds from terrestrial and marine plants and animals; biochemical studies, including biosynthesis and microbiological transformations; fermentation and plant tissue culture; the isolation, structure elucidation, and chemical synthesis of novel compounds from nature; and the pharmacology of compounds of natural origin.